Product Details

Uniprot No.

Target Names

SULT2A1

Alternative Names

Bile salt sulfotransferase (EC 2.8.2.14) (Dehydroepiandrosterone sulfotransferase) (DHEA-ST) (Hydroxysteroid Sulfotransferase) (HST) (ST2) (ST2A3) (Sulfotransferase 2A1) (ST2A1), SULT2A1, HST STD

Species Reactivity

Human

Immunogen

A synthesized peptide from human SULT2A1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

4C5

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

SULT2A1, also known as dehydroepiandrosterone sulfotransferase or bile salt sulfotransferase, plays a central role in phase II metabolism by catalyzing the sulfonation of hydroxysteroids and bile acids. This enzyme is predominantly expressed in the liver and adrenal glands, where it regulates the bioavailability of steroid hormones including DHEA, a precursor to both androgens and estrogens. Understanding SULT2A1 expression and activity is essential for researchers investigating steroid hormone metabolism, drug metabolism pathways, and hepatic function in both normal physiology and disease states.

This recombinant monoclonal antibody, clone 4C5, offers the reproducibility and consistency that demanding research applications require. Generated against a synthetic peptide derived from human SULT2A1, the antibody is produced using recombinant technology, ensuring sequence-defined specificity and eliminating the lot-to-lot variability often encountered with traditional hybridoma-derived antibodies. The rabbit IgG format provides excellent signal-to-noise characteristics across multiple detection platforms.

Validation studies demonstrate robust performance in immunohistochemistry, where the antibody successfully detects SULT2A1 in paraffin-embedded human liver tissue and liver cancer specimens at dilutions of 1:100. Immunofluorescence and flow cytometry applications have been validated using HepG2 cells, a well-established hepatocellular carcinoma line that endogenously expresses this sulfotransferase. The clear cytoplasmic staining pattern observed in these hepatic models aligns with the known subcellular localization of this metabolic enzyme.

This antibody serves as a valuable tool for researchers studying hepatic metabolism, steroid hormone regulation, drug biotransformation, and liver pathology, providing reliable detection of SULT2A1 across tissue-based and cell-based experimental workflows.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfonation of steroids and bile acids in the liver and adrenal glands. Mediates the sulfation of a wide range of steroids and sterols, including pregnenolone, androsterone, DHEA, bile acids, cholesterol and as well many xenobiotics that contain alcohol and phenol functional groups. Sulfonation increases the water solubility of most compounds, and therefore their renal excretion, but it can also result in bioactivation to form active metabolites. Plays an important role in maintening steroid and lipid homeostasis. Plays a key role in bile acid metabolism. In addition, catalyzes the metabolic activation of potent carcinogenic polycyclic arylmethanols.

Gene References into Functions

Kinetic analyses showed further differential catalytic efficiency and substrate affinity of the SULT2A1 allozymes, in comparison with wild-type SULT2A1. These findings provided useful information concerning the effects of genetic polymorphisms on the sulfating activity of SULT2A1 allozymes. PMID: 29671343
Polymorphisms of SULT2A1 gene is not associated with attention-deficit/hyperactivity disorder. PMID: 28367959
nine human SULT2A1 allozymes plus the wild-type SULT2A1 were found to display differential sulfating activity toward DHEA and tibolone. Kinetic analysis revealed that different SULT2A1 allozymes exhibited differential substrate affinity and catalytic efficiency toward the two substrates tested. PMID: 29392568
Mass spectrometry analysis and structural modeling supported a reaction mechanism which involves the isomerization of Delta(4)-3-ketosteroids from the keto form to an enol form, prior to being subjected to sulfation. Results derived from this study suggested a potential role of SULT2A1 as a Delta(4)-3-ketosteroid sulfotransferase in steroid metabolism PMID: 28782626
A theoretical study at the level of density functional theory (DFT) was performed to characterize noncovalent intermolecular interactions, especially hydrogen bond interactions, in the active site of enzyme human androsterone sulphotransferase. PMID: 27337388
Decreased SULT2A1 activity was found in the adrenal zona reticularis in Alzheimer's disease patients. PMID: 26680489
PSC is characterized by disease-specific impairment of SULT2A1 expression following PXR activation, a phenomenon which is not noted in PBC, and may account for the impaired hepatoprotection in PSC. PMID: 26504856
It metabolizes breast cancer drugs like afinoxifene and endoxifen by sulfation. PMID: 26169578
The substrate, such as lithocholic acid (LCA), participated in regulating the structure and flexibility of sulfotransferase actively rather than merely being selected passively. PMID: 26149755
Our results show that SULT2A1 is important in the first trimester; particularly in the adrenals PMID: 25802089
molecular dynamic simulations were used to investigate the effect of ligands (cofactor and substrate) on the thermo-denaturation process of hSULT2A1 PMID: 25750022
results established human SULT2A1 as a novel LXRalpha target gene; the expression of LXRalpha is a potential predictor for the expression of SULT2A1 in human liver PMID: 25028566
It turns out the protective effect of DHEA was significantly decreased when Wnt/beta-catenin signaling was activated, while inactivating Wnt/beta-catenin signaling enhanced the effects of DHEA PMID: 25065588
The complete kinetic mechanism of human SULT2A1. PMID: 25056952
Fetal inflammatory response syndrome and funisitis are associated with an elevation of umbilical cord plasma concentrations of soluble sulfotransferase (ST)2. PMID: 23488731
Depression following hip fracture with poor long term recovery is associated with a higher serum cortisol:DHEAS ratio. PMID: 23773910
Genetic variants in SULT2A1 do not predispose to polycystic ovary syndrome. Although a variant in SULT2A1 decreased the DHEAS to DHEA ratio, no changes in other androgenic hormone levels were observed. PMID: 23861462
formation of disulfide bonds in hSULT2A1 is a potentially important reversible mechanism for alterations in the rates of sulfation of both endogenous and xenobiotic substrates PMID: 23444386
These data show an age-related association of polymorphisms in the SULT2A1 gene with lower dehydroepiandrosterone sulfate, suggesting that these polymorphisms may affect dehydroepiandrosterone sulfate concentration in adults. PMID: 23436881
SULT2A1 as a novel ROR-alpha and ROR-gamma target gene. PMID: 23211525
Genetic variants of SULT2A1 do not appear to have an effect on individual DHEA and DHEAS concentrations or the DHEA/DHEAS ratio as a marker of DHEA sulfonation capacity PMID: 23132913
A crystallographic and biochemical description of the molecular linkages that couple nucleotide binding and SULT2A1 isomerization. PMID: 23362278
The simulations predict that the active-site "cap," which covers both the nucleotide and acceptor binding sites, opens and closes in response to nucleotide. PMID: 23256751
Common polymorphisms at POR, SULT2A1 or HSD11B1 were not associated with PA in a Finnish Caucasian population. PMID: 22445027
Serum DHEAS levels are increased in patients with non-alcoholic fatty liver disease with elevated alanine aminotransferase levels. PMID: 21841322
Hydroxysteroid sulfotransferase SULT2A1 is suppressed by the estrogen related receptor alpha PMID: 21513704
The sulfation kinetics of dehydroepiandrosterone and 15 human bile acids by SULT2A1, was characterized. PMID: 20102295
Crystal structure of human dehydroepiandrosterone sulphotransferase in complex with substrate PMID: 11988089
Both steroidogenic factor 1 and GATA-6 were positive regulators of SULT2A1 promoter constructs PMID: 15388788
lowered expression in hepatocellular carcinoma tissues involved in metabolism and/or inactivation of sex steroids is consistent with a regulatory role SULT2A1 in the development and/or tumor cell differentiation and progression of human HCC PMID: 15713538
Estrogen receptor alphaeffects on SULT2A1 were greater than the stimulation seen in response to steroidogenic factor 1 (SF1). PMID: 15878968
The SULT2A1 is the major endogenous enzyme responsible for sulfation of the tibolone metabolites in human postmenopausal tissues. PMID: 16360722
the SULT2A1 gene is mediated by SF1 and indirectly regulated by T(3) PMID: 16469813
No significant association of the presence of the different SULT2A1 alleles with the occurrence of prostate cancer in black men was detected. PMID: 16617014
Methotrexate induction of hSULT2A1 is mediated through hCAR. PMID: 17276571
genetic evidence suggesting a potential role of SULT2A1, but not STS, in the inherited adrenal androgen excess of polycystic ovary syndrome PMID: 17426092
Two amino acids are identified and characterized for the substrate inhibition of SULT2A1. PMID: 18042734
In cortices attached to adrenocortical adenomas, SULT2A1 mRNA expression was present mainly in zona reticularis and extended to zona fasciculata. PMID: 18505908
Sulfation of polychlorinated phenols catalyzed by hSULT2A1 may be a significant component of their metabolism in humans. PMID: 18656962
Testosterone and insulin appear to be modulators of adrenal androgen production in this human adrenocortical cell model. PMID: 18684447
Demonstrate that hSULT2A1 is responsible for the N-sulfation of quinolones and possibly other therapeutic drugs in humans. PMID: 19420132

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Subcellular Location

Cytoplasm.

Protein Families

Sulfotransferase 1 family

Tissue Specificity

Liver, adrenal and at lower level in the kidney. Is present in human fetus in higher level in the adrenal than the liver and the kidney.

Database Links

HGNC: 11458

OMIM: 125263

KEGG: hsa:6822

STRING: 9606.ENSP00000222002

UniGene: Hs.515835

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Code	CSB-RA290314A0HU
Size	US$210
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Image	IHC image of CSB-RA290314A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA290314A0HU diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of HepG2 cell with CSB-RA290314A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing HepG2 cells stained with CSB-RA290314A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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SULT2A1 Recombinant Monoclonal Antibody

Product Details

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Target Background