Product Details

Uniprot No.

P19532

Target Names

TFE3

Alternative Names

Transcription factor E3 (Class E basic helix-loop-helix protein 33) (bHLHe33), TFE3, BHLHE33

Species Reactivity

Human

Immunogen

A synthesized peptide from human TFE3 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

6E8

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, FC

Recommended Dilution

Application	Recommended Dilution
FC	1:50-1:200

Protocols

ELISA Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

TFE3 belongs to the MiT/TFE family of basic helix-loop-helix transcription factors that play essential roles in lysosomal biogenesis, autophagy regulation, and cellular metabolism. This transcription factor has gained considerable research attention due to its involvement in translocation renal cell carcinomas, where TFE3 gene fusions serve as diagnostic markers, and its emerging significance in metabolic disease research and immune cell function.

This recombinant monoclonal antibody, clone 6E8, offers researchers the reproducibility advantages inherent to recombinant technology. Because the antibody sequence is defined and produced from a stable expression system, you can expect consistent performance across different lots, eliminating the variability concerns that can complicate long-term studies or multi-site collaborations. The antibody is raised in rabbit against a synthesized peptide derived from human TFE3 protein and purified by affinity chromatography to ensure high specificity.

Validation for flow cytometry demonstrates clear detection of TFE3 in HepG2 cells, a human hepatocellular carcinoma line. The validation protocol employed fixation with formaldehyde followed by permeabilization with Triton X-100, appropriate for detecting this nuclear transcription factor. The overlay histogram shows distinct separation between the TFE3-stained population and the isotype control, confirming specific intracellular detection at the recommended dilution range of 1:50 to 1:200.

This antibody is well-suited for researchers investigating TFE3 biology in cancer, autophagy, and metabolic regulation contexts. The flow cytometry validation makes it particularly useful for quantifying TFE3 expression levels across cell populations or screening for TFE3 activation states in response to nutrient stress or lysosomal perturbation.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Transcription factor that acts as a master regulator of lysosomal biogenesis and immune response. Specifically recognizes and binds E-box sequences (5'-CANNTG-3'); efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. Involved in the cellular response to amino acid availability by acting downstream of MTOR: in the presence of nutrients, TFE3 phosphorylation by MTOR promotes its cytosolic retention and subsequent inactivation. Upon starvation or lysosomal stress, inhibition of MTOR induces TFE3 dephosphorylation, resulting in nuclear localization and transcription factor activity. In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer. It also binds very well to a USF/MLTF site. May regulate lysosomal positioning in response to nutrient deprivation by promoting the expression of PIP4P1. Acts as a positive regulator of browning of adipose tissue by promoting expression of target genes; mTOR-dependent phosphorylation promotes cytoplasmic retention of TFE3 and inhibits browning of adipose tissue. Maintains the pluripotent state of embryonic stem cells by promoting the expression of genes such as ESRRB; mTOR-dependent nuclear exclusion promotes exit from pluripotency. Required to maintain the naive pluripotent state of hematopoietic stem cell; mTOR-dependent cytoplasmic retention of TFE3 promotes the exit of hematopoietic stem cell from pluripotency.

Gene References into Functions

TFE3 gene rearrangement is associated with Epithelioid Rich Perivascular Epithelioid Cell Neoplasm of the Bladder. PMID: 29531006
The data reveal that 4E-BP3 expression is controlled by the transcription factor TFE3 through a cis-regulatory element in the EIF4EBP3 gene promoter. PMID: 27319316
A Potential G-quadruplex-forming Sequence (PQS) in the intron 2 of the TFE3 gene suggested by in silico studies was confirmed. Increased and decreased formation of G-quadruplex occurred in presence of pyridostatin and antisense oligonucleotide respectively. Stable G-quadruplex formation affected biological processes and TFE3 splicing. G-quadruplex is behind TFE3 induced oncogenesis via translocation and mRNA splicing. PMID: 29138008
TFE3 may promote renal tumor growth by regulating cell cycle progression and activating the phosphatidylinositol 3kinase/AKT serine/threonine kinase 1/mTOR signaling pathway. PMID: 28713889
We report for the first time the presence of both TFE3 translocation and SDHB mutation in the same renal cell carcinoma tumor. PMID: 27910947
TFEB and TFE3 collaborate with each other in activated macrophages and microglia to promote efficient autophagy induction, increased lysosomal biogenesis, and transcriptional upregulation of numerous proinflammatory cytokines PMID: 27171064
Findings indicated that TFE3 was an important hypoxia-induced transcriptional factor in HNSCC. PMID: 26872381
results reveal that PRCC-TFE3 dual-fusion FISH probe is an efficient and concise technique for diagnosing PRCC-TFE3 RCC in paraffin-embedded tissue PMID: 28949976
Case Report: Melanotic Xp11 renal cell carcinoma with ARID1B-TFE3 gene fusion. PMID: 28877054
Studies identified TFEB and TFE3 as master modulators of stress response notably in the lysosomal biogenesis and autophagy with capability to upregulate hundreds of genes involved in intracellular clearance, catabolism, metabolic processes, and cellular homeostasis. PMID: 27892768
Ovarian sclerosing stromal tumor strongly overexpress TFE3. PMID: 28982854
Describe the unusual morphology and expanded the morphologic spectrum of SFPQ/PSF-TFE3 renal cell carcinomas. PMID: 28315422
Despite TFE3 over expression, TFE3 genetic alterations are less likely to be a major molecular event driving tumorigenesis in hepatic angiolipomas. PMID: 27865795
we analyze 60 Xp11 translocation cancers by fluorescence in situ hybridization using custom bacterial artificial chromosome probes to establish their TFE3 fusion gene partner PMID: 26975036
These results show that ASPL-TFE3 regulates cell cycle progression and induces cellular senescence by up-regulating p21 expression. PMID: 27673450
RBM10-TFE3 is a recurrent gene fusion in Xp11 translocation renal cell carcinoma. PMID: 28296677
Case Report: melanotic PEComa of the sinonasal mucosa with NONO-TFE3 fusion. PMID: 28009605
Xp11 translocation renal cell carcinomas with RBM10-TFE3 gene fusion demonstrating melanotic features and overlapping morphology with t(6;11) RCC. PMID: 28288037
The essential parts of the Golgi stress response from the perspective of the organelle autoregulation. The pathways of the mammalian Golgi stress response have been identified, specifically the TFE3 pathway. PMID: 28179603
Female patients with Xp11.2 renal cell carcinoma in adults exhibit a high incidence compared to male, but not in children. Comparable clinical characteristics including incidence of distant and lymphatic metastases, tumor stage and prognosis is presented between male and female as well as between children and adults PMID: 27401463
RBM10-TFE3 fusion variant (from chromosome X paracentric inversion), therefore, appears to be a recurrent molecular event in Xp11.2 RCCs. RBM10-TFE3 fusion should be added in the list of screened fusion transcripts in targeted molecular diagnostic multiplex RT-PCR PMID: 26998913
The recently identified group of Xp11.2 translocation/TFE gene fusion renal cell carcinomas (Xp11.2/TFE RCC) affect approximately 15 % of patients under 45 years old with renal cell carcinoma. PMID: 27255396
Akt modulates nuclear translocation of TFE3. PMID: 28165011
over-expression of TFE3 ameliorates the steatosis in hepatocytes exposed to free fatty acids (FFAs). The effects of TFE3 on hepatic steatosis are dependent on the autophagy-induced lipophagy and PGC1alpha-mediated fatty acid beta-oxidation. PMID: 26999124
CT features including diameter, boundary, attenuation, nature, and circular calcification of the tumor, combined with demographic information and symptoms, may be useful to differentiate Xp11.2 RCC from different subtypes of PRCC. PMID: 26636097
TFEB and TFE3 are novel components of the integrated stress response PMID: 26813791
validates the utility of TFE3 break-apart fluorescence in situ hybridization on formalin-fixed paraffin-embedded tissue sections for diagnosis and confirmation of Xp11.2 translocation RCCs and alveolar soft part sarcoma PMID: 26415891
Studies indicate that alveolar soft part sarcoma showed a specific chromosomal alteration, der(17)t(X:17)(p11:q25), resulting in the fusion of the TFE3 transcription factor gene (Xp11) with alveolar soft part sarcoma critical region 1 (ASPSCR1) at 17q25. PMID: 26516944
TMED6-COG8 chimera might act as a novel diagnostic marker in TFE3 translocation renal cell carcinoma. PMID: 26045774
We report a case of RCC with Xp11.2 translocation in a 17 year old boy who is a survivor of stage 4 neuroblastoma 13 years earlier. PMID: 25551622
Case Report: renal carcinoma associated with Xp11.2 translocations/ TFE3 gene fusions diagnosed using urine cytology. PMID: 25227872
Renal cell carcinoma (RCC) associated with Xp11.2 translocation/TFE3 gene fusion (Xp11.2 RCC) was introduced as a genetically distinct entity into the World Health Organization classification of renal neoplasms in 2004. PMID: 25955396
TFE3-GASE pathway is one of the regulatory pathways of the mammalian Golgi stress response PMID: 25399611
PSF/SFPQ is a very common gene fusion partner in TFE3 rearrangement-associated perivascular epithelioid cell tumors (PEComas) and melanotic Xp11 translocation renal cancers. PMID: 26274027
Review of the role of gene fusions involving TFE3 and TFEB in carcinogenesis in sporadic renal cell carcinoma. PMID: 25048860
Case Report: PEComas of the cervix with TFE gene rearrangement. PMID: 25337301
Typical Xp11-RCC manifests as an advanced, solid renal mass with mild persistent enhancement, a prevalence of intertumor hemorrhage/calcification, and a cortical epicenter location. PMID: 24136829
The purpose of this study was to compare the MDCT features of renal cell carcinoma (RCC) associated with Xp11.2 translocation and TFE3 gene fusion (Xp11 RCC) and papillary RCC PMID: 25714283
Report TFE3 translocation-associated perivascular epithelioid cell neoplasms of the gynecological tract. PMID: 25517951
A conditional expression in mice of the fusion gene ASPSCR1-TFE3 from human alveolar soft part sarcoma (ASPS) generated a model that recapitulates the human tumor histologically and by expression profile. PMID: 25453902
study presents 3 cases of perivascular epithelioid cell tumor(PEComa) with TFE3 gene abnormalities; unusual cases of PEComa with TFE3 gene rearrangement might present malignant histological features and aggressive clinical behavior PMID: 25239799
Xp11.2 translocation/TFE3 gene fusion renal cell carcinoma (RCC) is the predominant form of pediatric RCC, associated with advanced stage at presentation. Patients with N + M0 maintained a favorable prognosis following surgery alone. PMID: 24726108
Report TFE3 rearrangement in Xp11.2 renal cell carcinoma and alveolar soft part sarcoma. PMID: 23828314
overexpression of TFE3 triggered lysosomal exocytosis and resulted in cellular clearance in a cellular model of a lysosomal storage disorder, Pompe disease, thus identifying TFE3 as a potential therapeutic target for the treatment of lysosomal disorders PMID: 24448649
Expression in 16 TFE3 translocated. PMID: 24339735
Case Report: bilateral renal cell carcinoma in a patient undergoing long-term dialysis, which showed false-positive immunoreactivity for TFE3 immunostaining. PMID: 24228124
cutaneous PEComas consistently lack TFE3 expression PMID: 23711163
In summary, we are reporting a novel subset of EHE occurring in young adults, showing a distinct phenotype and YAP1-TFE3 fusions. PMID: 23737213
TFE3 FISH is highly useful in renal tumor consultation material, often resolving cases with equivocal TFE3 immunohistochemistry results. PMID: 23715164
Our results support the clinical application of a TFE3 break-apart FISH assay for diagnosis and confirmation of Xp11.2 Renal cell carcinoma PMID: 23598965

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Involvement in disease

A chromosomal aberration involving TFE3 is found in patients with alveolar soft part sarcoma. Translocation t(X;17)(p11;q25) with ASPSCR1 forms a ASPSCR1-TFE3 fusion protein.

Subcellular Location

Cytoplasm, cytosol. Nucleus.

Protein Families

MiT/TFE family

Tissue Specificity

Ubiquitous in fetal and adult tissues.

Database Links

HGNC: 11752

OMIM: 314310

KEGG: hsa:7030

STRING: 9606.ENSP00000314129

UniGene: Hs.730740

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Code	CSB-RA085988A0HU
Size	US$210
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Image	Overlay Peak curve showing HepG2 cells stained with CSB-RA085988A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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TFE3 Recombinant Monoclonal Antibody

Product Details

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Target Background