THEMIS Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA262013A0HU
Size	US$210
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Image	IHC image of CSB-RA262013A0HU diluted at 1:50 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.30% DAB. Immunofluorescence staining of HepG2 with CSB-RA262013A0HU at 1:40, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 503-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing Jurkat cells stained with CSB-RA262013A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Target Background

Product Details

Uniprot No.

Q8N1K5

Target Names

THEMIS

Alternative Names

Protein THEMIS (Thymocyte-expressed molecule involved in selection), THEMIS, C6orf190 C6orf207

Species Reactivity

Human

Immunogen

A synthesized peptide derived from Human THEMIS

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

7G7

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

THEMIS plays a central role in T cell development, functioning as a critical signaling molecule during thymocyte selection processes that shape the adaptive immune repertoire. This protein participates in T cell receptor signal transduction pathways that determine whether developing thymocytes undergo positive or negative selection, making it an essential target for researchers investigating thymic education, T cell maturation, and immune system development.

This recombinant monoclonal antibody, generated against a synthetic peptide derived from human THEMIS, offers the reproducibility and consistency that demanding immunological studies require. As a sequence-defined reagent produced through recombinant technology, clone 7G7 eliminates the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations. The rabbit IgG format provides strong signal amplification while maintaining specificity for your target.

Validation across multiple detection platforms demonstrates this antibody's versatility in your experimental workflows. Immunohistochemistry studies in paraffin-embedded human tonsil tissue reveal clear staining using a polymer detection system with citrate buffer antigen retrieval at dilutions of 1:50-1:200. For cellular localization studies, immunofluorescence analysis in HepG2 cells confirms reliable detection of THEMIS with maintained morphological context. Flow cytometry validation using Jurkat cells, a T cell line where THEMIS expression is particularly relevant, shows distinct positive population shifts compared to isotype controls, supporting quantitative expression analysis in suspension cell applications.

Whether you are examining thymocyte development, characterizing T cell signaling pathways, or exploring stem cell differentiation toward lymphoid lineages, this antibody provides a dependable tool for advancing your immunology research with confidence in your results.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells. Regulates T-cell development through T-cell antigen receptor (TCR) signaling and in particular through the regulation of calcium influx and phosphorylation of Erk.

Gene References into Functions

we have demonstrated mutually exclusive use of exon 1 and 2 amongst 16 novel THEMIS isoforms. We also show inverse correlation between THEMIS expression in human CD4+ T-cells and dosage of the multiple sclerosis risk allele at rs13204742, driven by reduced expression of exon 1- containing isoforms PMID: 27438997
we found that THEMIS directly regulated the catalytic activity of the tyrosine phosphatase SHP-1. PMID: 28250424
the THEMIS:SHP complex dampens early TCR signalling by a previously unknown molecular mechanism that favours T-cell survival. PMID: 25535246
THEMIS showed higher mRNA expression in active celiac disease compared with treated patients and controls. PMID: 23820479
THEMIS binding to GRB2 was required for thymocyte development. PMID: 23460737
Themis1 exhibits the same potential to restore T cell development and T cell receptor-mediated signaling in Themis1-deficient mice. PMID: 22732588
SPOP mutations may define a new molecular subtype of prostate cancer. PMID: 22610119
specific role for THEMIS in early TCR signalosome function PMID: 21189249
Observational study of gene-disease association. (HuGE Navigator) PMID: 19913121
Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator) PMID: 20628086
Observational study of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 20095854
Functional characterization of the homologous mouse gene. PMID: 19597498
Functional analysis of the mouse gene by knockout, and comparison of the mouse and human proteins. PMID: 19597499

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Subcellular Location

Cytoplasm. Nucleus.

Protein Families

Themis family

Database Links

HGNC: 21569

OMIM: 613607

KEGG: hsa:387357

UniGene: Hs.661756

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Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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