TIMM44 Recombinant Monoclonal Antibody

TIMM44 serves as an essential component of the mitochondrial protein import machinery, functioning as a membrane anchor that tethers mtHsp70 to the inner membrane translocase complex. This positioning is critical for the ATP-dependent translocation of preproteins into the mitochondrial matrix, making TIMM44 a key player in mitochondrial biogenesis and cellular metabolism research.

This recombinant monoclonal antibody, generated from clone 25G3, offers the reproducibility that demanding mitochondrial studies require. Because the antibody sequence is defined and production is cell-based, you can expect consistent performance across experiments and projects, eliminating the lot-to-lot variability that can complicate longitudinal studies or multi-site collaborations.

Validation data demonstrates reliable detection across multiple experimental platforms. In western blot applications, the antibody produces a clean band at the predicted 51 kDa molecular weight, confirmed in human cell lines including K562, HT-29, and COLO-205, as well as rat liver tissue lysate, supporting cross-species reactivity for comparative studies. Immunohistochemistry validation in paraffin-embedded human liver and prostate cancer tissues shows specific staining patterns suitable for tissue-based investigations. For subcellular localization studies, immunofluorescence testing in U251 glioblastoma cells demonstrates compatibility with standard fixation and permeabilization protocols.

The antibody's performance across ELISA, western blot, immunohistochemistry, and immunofluorescence applications provides flexibility for researchers investigating mitochondrial import dynamics, metabolic dysfunction, or the emerging connections between mitochondrial protein homeostasis and disease states. Whether you're characterizing mitochondrial function in cancer models or exploring metabolic pathways, this antibody delivers the specificity and consistency your experiments demand.