TOMM22 Recombinant Monoclonal Antibody

TOMM22 serves as an essential component of the translocase of the outer mitochondrial membrane complex, functioning as a central receptor that facilitates the import of nuclear-encoded proteins into mitochondria. This protein plays a critical role in maintaining mitochondrial proteostasis and has become increasingly relevant in studies examining mitochondrial dysfunction, cellular metabolism, and neurodegenerative disease mechanisms.

This recombinant monoclonal antibody, generated in rabbit using clone 7C11, offers the reproducibility and consistency that demanding research applications require. Because recombinant antibodies are produced from defined genetic sequences rather than traditional hybridoma methods, researchers benefit from lot-to-lot uniformity that supports longitudinal studies and ensures experimental comparability across different time points and laboratories.

Validation testing demonstrates robust performance across multiple experimental platforms. In western blot applications, this antibody reliably detects TOMM22 across a diverse panel of human cell lines including 293, HeLa, L02, HepG2, Raji, Jurkat, and K562 whole cell lysates at dilutions ranging from 1:500 to 1:5000. The observed band size of approximately 18 kDa, slightly higher than the predicted 16 kDa molecular weight, likely reflects post-translational modifications characteristic of the native protein. For immunohistochemistry, validation in paraffin-embedded human kidney tissue at 1:50 to 1:200 dilutions confirms suitability for tissue-based investigations of mitochondrial distribution and abundance.

Whether investigating mitochondrial import machinery, characterizing organelle dynamics, or exploring metabolic reprogramming in disease models, this antibody provides a dependable tool for researchers studying mitochondrial biology and its broader implications in cellular health and pathology.