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| Code | CSB-RA277640A0HU |
| Size | US$210 |
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| Application | Recommended Dilution |
|---|---|
| WB | 1:500-1:5000 |
| IHC | 1:50-1:200 |
TOMM22 encodes TOM22, an essential component of the protein translocation complex (Tom complex) of the mitochondrial outer membrane. TOM22 serves as the central receptor for both presequence precursors and carrier precursors. The N-terminal domain of TOM22 functions as a preprotein receptor in cooperation with TOM20. In addition to functioning as an import receptor, TOM22 forms a conducting channel with TOM40, a major component of the general insertion pore. TOM22 deficiency might induce cardiomyocyte dysfunction by interfering with cardiac mitochondrial Ca2+ import.
The vectors expressing anti-TOMM22 antibody were constructed as follows: immunizing an animal with A synthesized peptide derived from human TOMM22, isolating the positive splenocyte and extracting RNA, obtaining DNA by reverse transcription, sequencing and screening TOMM22 antibody gene, and amplifying heavy and light chain sequence by PCR and cloning them into plasma vectors. After that, the vector clones were transfected into the mammalian cells for production. The product is the recombinant TOMM22 antibody. Recombinant TOMM22 antibody in the culture medium was purified using Affinity-chromatography. It can react with TOMM22 protein from Human and is used in the ELISA, WB, IHC.There are currently no reviews for this product.
