UQCRC2 Recombinant Monoclonal Antibody

UQCRC2 serves as a core structural component of mitochondrial Complex III, the cytochrome b-c1 complex that plays an essential role in the electron transport chain. As a key player in oxidative phosphorylation, this protein is central to cellular energy metabolism and has emerged as an important focus in cancer biology, metabolic disorders, and mitochondrial dysfunction research.

This recombinant monoclonal antibody, generated from clone 2F6, offers the reproducibility and consistency that mitochondrial research demands. Because the antibody sequence is defined and produced recombinantly in rabbit host cells, you can expect uniform performance across experiments and over time, eliminating the lot-to-lot variability that can complicate longitudinal studies or multi-site collaborations.

Validation testing demonstrates reliable detection of UQCRC2 at the expected 49 kDa molecular weight across multiple human cell lines, including HepG2 hepatocellular carcinoma, 293 embryonic kidney, U251 glioblastoma, and MCF-7 breast cancer cells. This broad validation across diverse cellular backgrounds confirms robust performance for western blot applications, with recommended dilutions ranging from 1:500 to 1:5000 depending on your experimental system.

For researchers investigating tissue-level expression patterns, immunohistochemistry validation in paraffin-embedded human heart and liver tissues shows clear staining at 1:100 dilution using standard citrate buffer antigen retrieval. These metabolically active tissues provide excellent positive controls for establishing detection in your own samples.

Whether you're investigating mitochondrial bioenergetics, exploring metabolic reprogramming in cancer, or studying signal transduction pathways linked to cellular respiration, this antibody provides a dependable tool for characterizing UQCRC2 expression in human samples.