UQCRFS1 Recombinant Monoclonal Antibody

UQCRFS1, also known as the Rieske iron-sulfur protein, serves as an essential component of mitochondrial Complex III within the electron transport chain. This protein facilitates electron transfer from ubiquinol to cytochrome c, making it fundamental to oxidative phosphorylation and cellular energy production. Researchers investigating mitochondrial function, metabolic disorders, and bioenergetics rely on precise detection of this target to understand respiratory chain dynamics and dysfunction.

This recombinant monoclonal antibody, clone 4F10, offers the consistency and reproducibility that demanding mitochondrial research requires. Developed using recombinant technology with a defined sequence, it eliminates the lot-to-lot variability that can complicate longitudinal studies or multi-site collaborations. The rabbit host and affinity-chromatography purification ensure high specificity for your target across experimental platforms.

Validation data demonstrates robust performance across multiple applications. Western blot analysis at 1:1000 dilution reveals a clean 23 kDa band consistent with the processed form of UQCRFS1, detected across diverse human cell lines including MCF-7, U937, HeLa, HEK293T, HL-60, and HepG2. Cross-species reactivity extends to mouse tissues, with successful detection in heart and liver lysates, supporting translational studies between human cell models and murine systems. Immunohistochemistry validation in paraffin-embedded human breast cancer and stomach tissue at 1:100 dilution confirms utility for tissue-based investigations. Flow cytometry analysis in MCF-7 cells demonstrates clear positive signal separation from isotype controls, enabling intracellular protein quantification studies.

Whether you are characterizing mitochondrial biogenesis, investigating tumor metabolism, or exploring respiratory chain deficiencies, this antibody provides the workflow flexibility and reliable performance needed for meaningful experimental outcomes.