USP39 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA073613A0HU
Size	US$210
	Order now
Image	Western Blot , Positive WB detected in:HEK293 whole cell lysate(30µg) All lanes: USP39 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 65 kDa Observed band size:65 kDa Exposure time：120s Overlay Peak curve showing Hela cells stained with CSB-RA073613A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Uniprot No.

Q53GS9

Target Names

USP39

Alternative Names

65K; CGI 21; CGI-21; FLJ33136; HSPC332; Inactive ubiquitin specific peptidase 39; Inactive ubiquitin-specific peptidase 39; MGC75069; PRO2855; S. CEREVISIAE, HOMOLOG OF SAD1; SAD1; SAD1 homolog; Small nuclear ribonucleoprotein 65kDa (U4/U6.U5); snRNP ASSEMBLY DEFECTIVE 1; SnRNP assembly defective 1 homolog; SNRNP65; SNUT2_HUMAN; U4/U6.U5 tri snRNP associated 65 kDa protein; U4/U6.U5 tri snRNP associated protein 2; U4/U6.U5 tri-snRNP-associated 65 kDa protein; U4/U6.U5 tri-snRNP-associated protein 2; Ubiquitin specific peptidase 39; Ubiquitin specific protease 39; USP39

Species Reactivity

Human

Immunogen

A synthesized peptide from human USP39 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

25G11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function

Plays a role in pre-mRNA splicing as a component of the U4/U6-U5 tri-snRNP, one of the building blocks of the precatalytic spliceosome. Regulates AURKB mRNA levels, and thereby plays a role in cytokinesis and in the spindle checkpoint. Does not have ubiquitin-specific peptidase activity.

Gene References into Functions

The above findings suggest that USP39 plays a vital oncogenic role in the tumorigenesis of prostate cancer PMID: 26959883
Knockdown of USP39 expression in osteosarcoma cell line U2OS cell significantly decreased cell proliferation, impaired colony formation ability. A further analysis indicated suppression of USP39 arrested cell cycle progression at G2/M phase via p21 dependent way. In addition, the results of Annexin V/7-AAD staining suggested the knockdown of USP39 could promote U2OS cell apoptosis through PARP cleavage. PMID: 28403900
Data suggest that ZIP, USP39, Prp24/p100/SART3, and Prp43 associate to form complex instrumental in spliceosome assembly; ZIP regulates pre-mRNA splicing of USP39 independent of RNA binding; stable 35S tri-snRNP particles are enriched in Cajal body. (ZIP = zinc finger and G-patch domain-containing protein; SART3 = squamous cell carcinoma antigen recognised by T cells 3; Prp43 = RNA helicase Prp43) PMID: 28878014
High USP39 expression is associated with colon and lung cancer. PMID: 28154181
Data show that knockdown of ubiquitin specific peptidase 39 (USP39), a direct target of microRNA microRNA-133a, induced cell apoptosis through inhibition of proto-oncogene proteins c-akt (AKT) signaling pathway in pancreatic cancer (PC) cells. PMID: 28286270
Results show a significantly higher expression of USP39 in colorectal cancer tissues and cell lines and provide evidence that USP39 protein plays an important role in the growth and metastasis of colorectal cancer mainly through Wnt/beta-catenin pathway. PMID: 28259917
shRNA-mediated knockdown of USP39 inhibited the growth and colony formation ability of GC cells. suppression of USP39 induced G2/M-phase arrest and increased the cleavage of PARP (Asp214). PMID: 27175747
USP39 is upregulated in melanoma tissues and that it is implicated in melanoma progression by regulating cell cycle and apoptosis via ERK1/2 signal pathways. PMID: 27456357
study indicates that USP39 may be functionally involved in lung cancer growth and act as a potential molecular target for human lung cancer diagnosis and treatment. PMID: 27629785
Our data indicate that USP39 knockdown inhibited the growth of HCC both in vitro and in vivo through G2/M arrest, which was partly achieved via the inhibition of FoxM1 splicing. PMID: 26081192
Inducible knockdown of USP39 or overexpression of USP39 in TNBC cells. PMID: 25812575
These results suggest that USP39 may act as an oncogenic factor in breast cancer and could be a potential molecular target for breast cancer gene therapy. PMID: 24126978
These observations suggest Usp39 to be involved in splicing of Aurora B and other mRNAs that are essential for proper spindle checkpoint function. PMID: 18728397

Hide All

Subcellular Location

Nucleus.

Protein Families

Peptidase C19 family

Database Links

HGNC: 20071

OMIM: 611594

KEGG: hsa:10713

STRING: 9606.ENSP00000312981

UniGene: Hs.654745

Promotions

CUSABIO Premium Active Proteins 20% OFF SALE

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Target Therapy

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

Join us with

Subscribe newsletter

Leave a message

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
Please enter the characters shown. Click the image to refresh if unclear.

Place an order now

I. Product details

Product name *

Code *

Size *

Quantity *

Message

II. Contact details

Name *

Email address *

Company/Organization

Phone

III. Ship To

Address *

City *

State/Province *

Zip/Post code *

Country *

Attn Name *

Phone# *

IV. Bill To Same as Shipping Address