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USP39 Recombinant Monoclonal Antibody

Datasheet
Code CSB-RA073613A0HU
Size US$210
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Image
  • Western Blot
    , Positive WB detected in:HEK293 whole cell lysate(30µg)
    All lanes: USP39 antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/40000 dilution
    Predicted band size: 65 kDa
    Observed band size:65 kDa
    Exposure time：120s
  • Overlay Peak curve showing Hela cells stained with CSB-RA073613A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Product Details

Uniprot No.
Q53GS9
Target Names
USP39
Alternative Names
65K; CGI 21; CGI-21; FLJ33136; HSPC332; Inactive ubiquitin specific peptidase 39; Inactive ubiquitin-specific peptidase 39; MGC75069; PRO2855; S. CEREVISIAE, HOMOLOG OF SAD1; SAD1; SAD1 homolog; Small nuclear ribonucleoprotein 65kDa (U4/U6.U5); snRNP ASSEMBLY DEFECTIVE 1; SnRNP assembly defective 1 homolog; SNRNP65; SNUT2_HUMAN; U4/U6.U5 tri snRNP associated 65 kDa protein; U4/U6.U5 tri snRNP associated protein 2; U4/U6.U5 tri-snRNP-associated 65 kDa protein; U4/U6.U5 tri-snRNP-associated protein 2; Ubiquitin specific peptidase 39; Ubiquitin specific protease 39; USP39
Species Reactivity
Human
Immunogen
A synthesized peptide from human USP39 protein
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone No.
25G11
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Form
Liquid
Tested Applications
ELISA, WB, FC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
FC 1:50-1:200
Protocols
ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

USP39 plays a critical role in pre-mRNA splicing as a core component of the U4/U6.U5 tri-snRNP complex, making it essential for proper spliceosome assembly and function. Despite containing a ubiquitin-specific peptidase domain, USP39 lacks catalytic activity and instead functions as a scaffold protein in splicing machinery. This dual identity has positioned USP39 as an important target in research exploring splicing regulation, cell cycle progression, and cancer biology, where aberrant splicing patterns frequently contribute to disease mechanisms.

This recombinant monoclonal antibody, clone 25G11, offers the reproducibility and consistency that demanding splicing research requires. Developed using recombinant technology with a defined sequence, this rabbit IgG eliminates the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations. The antibody was raised against a synthetic peptide derived from human USP39, ensuring precise epitope targeting.

Validation studies confirm reliable performance across multiple experimental platforms. Western blot analysis in HEK293 whole cell lysates demonstrates specific detection of USP39 at the predicted molecular weight of 65 kDa, with recommended dilutions ranging from 1:500 to 1:5000 providing flexibility for optimization. Flow cytometry validation using HeLa cells shows clear separation between specific signal and isotype control, supporting intracellular detection applications at dilutions of 1:50 to 1:200. The antibody is also validated for ELISA applications.

Supplied in a stabilized liquid format with glycerol for convenient storage at -20°C or -80°C, this affinity-purified antibody is well-suited for researchers investigating spliceosome biology, RNA processing mechanisms, or the emerging connections between splicing dysregulation and oncogenesis.

Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Plays a role in pre-mRNA splicing as a component of the U4/U6-U5 tri-snRNP, one of the building blocks of the precatalytic spliceosome. Regulates AURKB mRNA levels, and thereby plays a role in cytokinesis and in the spindle checkpoint. Does not have ubiquitin-specific peptidase activity.
Gene References into Functions
  1. The above findings suggest that USP39 plays a vital oncogenic role in the tumorigenesis of prostate cancer PMID: 26959883
  2. Knockdown of USP39 expression in osteosarcoma cell line U2OS cell significantly decreased cell proliferation, impaired colony formation ability. A further analysis indicated suppression of USP39 arrested cell cycle progression at G2/M phase via p21 dependent way. In addition, the results of Annexin V/7-AAD staining suggested the knockdown of USP39 could promote U2OS cell apoptosis through PARP cleavage. PMID: 28403900
  3. Data suggest that ZIP, USP39, Prp24/p100/SART3, and Prp43 associate to form complex instrumental in spliceosome assembly; ZIP regulates pre-mRNA splicing of USP39 independent of RNA binding; stable 35S tri-snRNP particles are enriched in Cajal body. (ZIP = zinc finger and G-patch domain-containing protein; SART3 = squamous cell carcinoma antigen recognised by T cells 3; Prp43 = RNA helicase Prp43) PMID: 28878014
  4. High USP39 expression is associated with colon and lung cancer. PMID: 28154181
  5. Data show that knockdown of ubiquitin specific peptidase 39 (USP39), a direct target of microRNA microRNA-133a, induced cell apoptosis through inhibition of proto-oncogene proteins c-akt (AKT) signaling pathway in pancreatic cancer (PC) cells. PMID: 28286270
  6. Results show a significantly higher expression of USP39 in colorectal cancer tissues and cell lines and provide evidence that USP39 protein plays an important role in the growth and metastasis of colorectal cancer mainly through Wnt/beta-catenin pathway. PMID: 28259917
  7. shRNA-mediated knockdown of USP39 inhibited the growth and colony formation ability of GC cells. suppression of USP39 induced G2/M-phase arrest and increased the cleavage of PARP (Asp214). PMID: 27175747
  8. USP39 is upregulated in melanoma tissues and that it is implicated in melanoma progression by regulating cell cycle and apoptosis via ERK1/2 signal pathways. PMID: 27456357
  9. study indicates that USP39 may be functionally involved in lung cancer growth and act as a potential molecular target for human lung cancer diagnosis and treatment. PMID: 27629785
  10. Our data indicate that USP39 knockdown inhibited the growth of HCC both in vitro and in vivo through G2/M arrest, which was partly achieved via the inhibition of FoxM1 splicing. PMID: 26081192
  11. Inducible knockdown of USP39 or overexpression of USP39 in TNBC cells. PMID: 25812575
  12. These results suggest that USP39 may act as an oncogenic factor in breast cancer and could be a potential molecular target for breast cancer gene therapy. PMID: 24126978
  13. These observations suggest Usp39 to be involved in splicing of Aurora B and other mRNAs that are essential for proper spindle checkpoint function. PMID: 18728397

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Subcellular Location
Nucleus.
Protein Families
Peptidase C19 family
Database Links

HGNC: 20071

OMIM: 611594

KEGG: hsa:10713

STRING: 9606.ENSP00000312981

UniGene: Hs.654745

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