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USP6 Recombinant Monoclonal Antibody

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Datasheet
Code CSB-RA025747MA1HU
Size $49.9
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Image
  • Overlay Peak curve showing jurkat cells stained with CSB-RA025747MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific at 1:200 dilution for 35min at 4℃.Control antibody (green line) was human IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details

Uniprot No.
P35125
Target Names
USP6
Alternative Names
Deubiquitinating enzyme 6 antibody; HRP1 antibody; Proto-oncogene TRE-2 antibody; TRE17 antibody; TRE2 antibody; Ubiquitin carboxyl-terminal hydrolase 6 antibody; Ubiquitin specific protease 6 antibody; Ubiquitin thiolesterase 6 antibody; Ubiquitin-specific-processing protease 6 antibody; UBP6_HUMAN antibody; USP6 antibody
Species Reactivity
Human
Immunogen
Recombinant Human USP6 protein
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
hIgG1
Clone No.
3C3G2
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, FC
Recommended Dilution
Application Recommended Dilution
FC 1:50-1:200
Protocols
ELISA Protocol
Flow Cytometry (FC) Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

USP6, also known as TRE-2 or TRE17, functions as a deubiquitinating enzyme that removes ubiquitin modifications from substrate proteins, thereby regulating their stability and activity. This proto-oncogene has garnered significant research attention due to its involvement in cellular signaling pathways and its association with specific bone tumors, particularly aneurysmal bone cysts, where chromosomal translocations drive aberrant USP6 expression. Understanding USP6 biology provides valuable insights into ubiquitin-mediated protein regulation and oncogenic mechanisms.

This recombinant monoclonal antibody, clone 3C3G2, offers researchers the reproducibility essential for longitudinal studies and multi-site collaborations. Because the antibody sequence is defined and production occurs through recombinant expression, you can expect consistent performance across lots, eliminating the variability often encountered with traditional hybridoma-derived reagents. The human IgG1 isotype format and affinity-chromatography purification ensure high specificity for your target detection needs.

Validation through flow cytometry demonstrates reliable detection of USP6 in human samples, with testing performed using Jurkat cells. In these experiments, cells were fixed and permeabilized to enable intracellular staining, revealing clear signal separation between the USP6 antibody and isotype control when used at a 1:100 dilution. This validation confirms the antibody's utility for intracellular flow cytometry applications studying human cell populations. The antibody has also been validated for ELISA, providing flexibility across different experimental platforms.

Researchers investigating ubiquitin pathway biology, bone tumor pathogenesis, or oncogenic signaling mechanisms will find this antibody a dependable tool for characterizing USP6 expression and exploring its functional roles in human cellular systems.

Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Deubiquitinase with an ATP-independent isopeptidase activity, cleaving at the C-terminus of the ubiquitin moiety. Catalyzes its own deubiquitination. In vitro, isoform 2, but not isoform 3, shows deubiquitinating activity. Promotes plasma membrane localization of ARF6 and selectively regulates ARF6-dependent endocytic protein trafficking. Is able to initiate tumorigenesis by inducing the production of matrix metalloproteinases following NF-kappa-B activation.
Gene References into Functions
  1. None of the genitourinary pseudosarcomatous myofibroblastic proliferations was found to harbour USP6 (0/12), ROS1 (0/8) or ETV6 (0/7) rearrangements PMID: 29617048
  2. we identified seven novel fusion partners for USP6 in nodular fasciitis, highlighting the importance of USP6 expression and promoter-swapping fusions in the etiology of this neoplasm PMID: 28752842
  3. Report the presence of USP6 rearrangements in a subset of cellular fibroma of tendon sheath. PMID: 27125357
  4. our studies highlight Jak1 as the first identified substrate for USP6, and they offer a mechanistic rationale for the clinical investigation of Jak and STAT3 inhibitors as therapeutics for the treatment of bone and soft tissue tumors along with other neoplasms driven by USP6 overexpression PMID: 27440725
  5. Molecular analyses revealed the presence and amplification of the novel PPPR6-USP6 gene fusion, which resulted in USP6 mRNA transcriptional upregulation. These findings further support the oncogenic role of the USP6 protease in mesenchymal neoplasia and expand the biologic potential of Nodular fasciitis PMID: 27113271
  6. It was shown that TRE17 activates the classical NF-kappa B pathway through an atypical mechanism that does not involve IkappaB degradation. Optimal activation of NF-kappa B by TRE17 required both catalytic subunits of IkappaB kinase. PMID: 22081069
  7. USP6 fluorescence in-situ hybridization is a useful ancillary test in cases where nodular fasciitis is a potential diagnostic consideration. PMID: 27271298
  8. the deubiquitylase ubiquitin-specific protease 6 (USP6) as a potent activator of Wnt signaling. USP6 enhances Wnt signaling by deubiquitylating Fzds, thereby increasing their cell-surface abundance. PMID: 27162353
  9. TRE17/USP6 regulates ubiquitylation and trafficking of cargo proteins that enter cells by clathrin-independent endocytosis PMID: 25179595
  10. 8 of the 9 giant cell reparative granulomas from hands and feet showed rearrangements of the USP6 gene compared with none of 8 gnathic lesions PMID: 24742829
  11. we discuss the clinicopathologic features, molecular pathology, and pathogenesis of ABC and nodular fasciitis in relation to USP6 PMID: 23769422
  12. identification of a USP6 gene rearrangement is helpful in making a diagnosis of nodular fasciitis. PMID: 23748914
  13. manipulating USP6 expression levels alters the ability of cells to migrate and to divide. Cell proliferation and progression through cytokinesis depend on USP6 expression PMID: 22188517
  14. TRE17 is sufficient to initiate tumorigenesis, identify MMPs as novel TRE17 effectors that likely contribute to aneurysmal bone cyst pathogenesis. PMID: 20418905
  15. TRE17 coprecipitated specifically with the active forms of Cdc42 and Rac1 in vivo. TRE17 is part of a novel effector complex for Cdc42 and Rac1, potentially contributing to their effects on actin remodeling. PMID: 12612085
  16. Complementation tests in yeasts indicate that Tre2 codes for a nonfunctional RabGAP. PMID: 14521938
  17. Deregulated USP6 transcription is associated with aneurysmal bone cyst PMID: 15026324
  18. primary aneurysmal bone cysts are mesenchymal neoplasms exhibiting USP6 and/or CDH11 oncogenic rearrangements PMID: 15509545
  19. TRE17 associates directly with Arf6 in its GDP- but not GTP-bound state. PMID: 15509780
  20. Tre2 oncogene seems to encode a nonfunctional Rab GAP. As regions flanking the TBC domain may be crucial for catalytic activity PMID: 16099424
  21. Ca2+/CaM has a role in regulating ubiquitination through direct interaction with TRE17 PMID: 16127172
  22. The lack of secondary structure of the region flanking the TBC domain in TRE2 may explain why this region plays a role in the lack of GAP activity, even when a potentially functional TBC domain is present. PMID: 17701273
  23. No USP6 rearrangements were found in cherubism or brown tumors. USP6 rearrangements were identified in 2 patients with myositis ossificans. PMID: 18265974

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Involvement in disease
A chromosomal aberration involving USP6 is a common genetic feature of aneurysmal bone cyst, a benign osseous neoplasm. Translocation t(16;17)(q22;p13) with CDH11. The translocation generates a fusion gene in which the strong CDH11 promoter is fused to the entire USP6 coding sequence, resulting in USP6 transcriptional up-regulation (PubMed:15026324).
Subcellular Location
Cell membrane. Cytoplasm. Endosome. Note=Localizes to the plasma membrane and to filamentous structures within the cell corresponding to ARF6 regulated tubular endosomes. Activation of RAC1 and CDC42 can direct the relocalization of USP6 to the plasma membrane in a manner that depends on the integrity of the actin cytoskeleton.
Protein Families
Peptidase C19 family
Tissue Specificity
Testis specific. Expressed in various cancer cell lines.
Database Links

HGNC: 12629

OMIM: 604334

KEGG: hsa:9098

STRING: 9606.ENSP00000250066

UniGene: Hs.448851

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