WDR1 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA147408A0HU
Size	US$210
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Image	IHC image of CSB-RA147408A0HU diluted at 1:100 and staining in paraffin-embedded human thyroid tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA147408A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing HepG2 cells stained with CSB-RA147408A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Target Background

Product Details

Uniprot No.

O75083

Target Names

WDR1

Alternative Names

Actin interacting protein 1 antibody; Actin-interacting protein 1 antibody; AIP1 antibody; D5Wsu185e antibody; NORI 1 antibody; NORI-1 antibody; WD repeat domain 1 antibody; WD repeat-containing protein 1 antibody; wdr1 antibody; WDR1_HUMAN antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human WDR1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

17D6

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

WDR1, also known as Actin-interacting protein 1, plays a critical role in actin cytoskeleton dynamics by enhancing cofilin-mediated actin filament disassembly. This function makes WDR1 essential for numerous cellular processes including cell migration, cytokinesis, and morphological changes, positioning it as a significant target for researchers investigating signal transduction pathways and cytoskeletal regulation.

This recombinant monoclonal antibody, clone 17D6, offers the consistency and reliability that demanding experimental workflows require. Generated through recombinant technology with a defined sequence, this antibody eliminates the lot-to-lot variability often encountered with traditional hybridoma-derived reagents. Researchers can confidently design long-term studies knowing that antibody performance will remain stable across multiple experiments and reorders.

Validation studies demonstrate robust performance across multiple applications. Immunohistochemistry testing in paraffin-embedded human tissues, including thyroid and lung cancer specimens, shows clear specific staining at 1:100 dilution using citrate buffer antigen retrieval. The antibody has also been validated for flow cytometry applications, with testing in HepG2 hepatocellular carcinoma cells showing distinct positive signal separation from isotype control when used at 1:100 dilution on fixed and permeabilized cells. These validated protocols provide researchers with established starting points for their own experimental optimization.

The unconjugated format offers flexibility for pairing with detection systems of choice, while the affinity-purified preparation ensures minimal background in sensitive applications. Whether investigating WDR1's role in cancer cell motility, examining its expression patterns across tissue types, or exploring its involvement in signal transduction networks, this antibody provides a dependable tool for advancing your research into actin dynamics and cellular regulation.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Induces disassembly of actin filaments in conjunction with ADF/cofilin family proteins. Enhances cofilin-mediated actin severing. Involved in cytokinesis. Involved in chemotactic cell migration by restricting lamellipodial membrane protrusions. Involved in myocardium sarcomere organization. Required for cardiomyocyte growth and maintenance. Involved in megakaryocyte maturation and platelet shedding. Required for the establishment of planar cell polarity (PCP) during follicular epithelium development and for cell shape changes during PCP; the function seems to implicate cooperation with CFL1 and/or DSTN/ADF. Involved in the generation/maintenance of cortical tension. Involved in assembly and maintenance of epithelial apical cell junctions and plays a role in the organization of the perijunctional actomyosin belt.

Gene References into Functions

STAT3 bound to the 5' upstream sequence (-1971 to -1964), a putative promoter region, of WDR1 gene, and its activation induced WDR1 overexpression in breast cancer cells. PMID: 27521604
MRTF-A-miR-206-WDR1 form feedback loop to regulate breast cancer cell migration. PMID: 28822708
homozygous missense L153F/L293F mutation in the actin regulatory gene WDR1 causes a new autoinflammatory disease in humans, with periodic fevers, immunodeficiency, and intermittent thrombocytopenia (PFIT). PMID: 27994071
These data suggest that WDR1 plays an important role in suppressing platelet activity, where it alters the actin cytoskeleton dynamics, and downregulation of WDR1 may contribute to the platelet-mediated pathogenesis of cardiovascular disease. PMID: 27609643
Mutations in WDR1 affect neutrophil morphology, motility, and function, causing a novel primary immunodeficiency PMID: 27557945
A/A" genotype of rs12498927 was associated with increased risk of gout. PMID: 27173277
Identify positive correlations between WDR1 and CLNK gene polymorphisms in Chinese-Tibetan gout populations. PMID: 26438387
Demonstrate a previously unanticipated role of Aip1 in regulating the structure and remodeling of intestinal epithelial junctions and early steps of epithelial morphogenesis. PMID: 25792565
When maternal environmental tobacco smoke was considered, fifteen of 135 single nucleotide polymorphism (SNPs) mapping to SLC2A9 and 9 of 59 SNPs in WDR1 gave P values approaching genome-wide significance. PMID: 24516586
Data suggest that anti-WD repeat domain 1 (WDR1) antibody could be applied for serological screening of papillary thyroid carcinoma (PTC) and undifferentiated thyroid carcinoma (UTC), and could be an efficient and inexpensive biomarker. PMID: 23215816
WD repeat-containing protein 1 was confirmed as being significantly overexpressed in interstitial fluid from ovarian carcinomas compared to healthy ovarian tissue. PMID: 23707566
Studies suggest that vascular endothelial growth factor may be responsible for upregulated P-glycoprotein expression. PMID: 23293323
The QKI RNA-binding proteins regulate oligodendrocyte differentiation by modulating the expression of AIP-1. PMID: 20631256
Studies indicate that CUL4 uses a large beta-propeller protein, DDB1, as a linker to interact with a subset of WD40 proteins. PMID: 19818632
AIP1/WDR1 could abort the severing/disassembly activity somewhat still carried out due to phosphorylated ADF/cofilin PMID: 15629458
Data indicates that Aip1 plays an important role in directional cell migration by restricting the stimulus-induced membrane protrusion to one direction via promoting cofilin activity. PMID: 18494608
Using shotgun mass spectrometry, we found this protein differentially expressed in the dorsolateral prefrontal cortex from patients with schizophrenia. PMID: 19165527

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Subcellular Location

Cytoplasm. Cytoplasm, cytoskeleton. Cell projection, podosome. Cell junction.

Protein Families

WD repeat AIP1 family

Tissue Specificity

Expressed in peripheral blood mononuclear cells (at protein level).

Database Links

HGNC: 12754

OMIM: 604734

KEGG: hsa:9948

STRING: 9606.ENSP00000427687

UniGene: Hs.128548

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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