Isotype
Secondary Antibody
Purification Method
The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Tested Applications
ELISA, WB, IHC, ICC
Recommended Dilution
Application |
Recommended Dilution |
ELISA |
1:500-1:10000 |
WB |
1:3000-1:5000 |
IHC |
1:50-1:500 |
ICC |
1:50-1:500 |
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.