Rabbit anti-Human IgG Fc Antibody;HRP conjugated

Datasheet
Code CSB-PA00540F0Rb
Product Type Secondary Antibody
Size US$169Purchase it in Cusabio online store
(only available for customers from the US)
Research Use Caprylic Acid Ammonium Sulfate Precipitation purified
Image
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    Western Blot
    Positive WB detected in Recombinant protein (80ng, 40ng, 20ng, 10ng)
    All lanes: Human IgG Fc antibody; HRP conjugated at 1:1000
    Predicted band size: 35 kDa
    Observed band size: 35 kDa

Immunogen Human IgG Fc fragment
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB; Recommended dilution: WB:1:500-1:5000
Relevance The fragment crystallizable region (Fc region) is the tail region of an antibody that interacts with cell surface receptors called Fc receptors and some proteins of the complement system. This property allows antibodies to activate the immune system. InIgG, IgA and IgD antibody isotypes, the Fc region is composed of two identical protein fragments, derived from the second and third constant domains of the antibody two heavy chains; IgM and IgE Fc regions contain three heavy chain constant domains (CH domains 2–4) in each polypeptide chain. The Fc regions of IgGs bear a highly conserved N-glycosylation site. Glycosylation of the Fc fragment is essential for Fc receptor-mediated activity. The N-glycans attached to this site are predominantly core-fucosylated diantennary structures of the complex type. In addition, small amounts of these N-glycans also bear bisecting GlcNAc and α-2,6 linked sialic acid residues.
Form Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Conjugate Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Storage Buffer Liquid
Purification Method HRP conjugated
Isotype IgG
Clonality Polyclonal
Alias fragment crystallizable region
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
References
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