6*His Monoclonal Antibody

6*His-tag (hexahistidine) is a widely utilized 6-amino-acid sequence (HHHHHH) in recombinant protein technology. As a versatile affinity tag, it enables efficient purification, detection, and immobilization of target proteins expressed in bacterial, yeast, or mammalian systems, playing a crucial role in structural and functional genomics research.

This mouse 6*His monoclonal antibody (IgG2a isotype) is generated against a synthetic 6xHis peptide conjugated to KLH and subsequently purified via Protein A affinity chromatography, ensuring high purity (>95%). It demonstrates robust immunoreactivity and exceptional specificity across multiple assay platforms.

Rigorous in-house validation confirms its efficacy in Western Blot (WB) and Enzyme-Linked Immunosorbent Assay (ELISA) applications. In WB experiments, the antibody detects His-tagged fusion proteins with high sensitivity, clearly identifying concentrations as low as 2.5 ng, with the observed band sizes precisely aligning with the predicted 55 kD and 60 kD molecular weights for different constructs.

As a rigorously validated immunological tool, this antibody serves as a critical reagent for confirming recombinant protein expression, facilitating affinity purification, and supporting a wide range of downstream biochemical analyses. Cited in multiple peer-reviewed scientific publications, this CUSABIO monoclonal antibody is backed by comprehensive technical support, ensuring dependable and reproducible results to accelerate your cutting-edge research.