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| Code | CSB-MA000101M0m |
| Size | US$120 |
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| Application | Recommended Dilution |
|---|---|
| WB | 1:1000-1:640000 |
| IP | 2µl-5µl |
The mouse is immunized with synthetic peptide GLNDIFEAQKIEWHE to obtain the B cells. Fusing mouse B cells with myeloma cells to obtain hybridomas, selecting the hybridoma cell line that produces the Avi-Tag antibody, and then culturing the selected hybridoma in the mouse abdominal cavity. The Avi-Tag monoclonal antibody is from the mouse ascites using protein G affinity chromatography, resulting in a purity of over 95%. This unconjugated IgG2a antibody is ideal for detecting the all Avi-Tagged proteins in ELISA, WB, and IP applications. The Avi-Tag is a short peptide sequence (GLNDIFEAQKIEWHE) that can be genetically encoded onto a protein of interest.
The Avi-Tag sequence is recognized by the biotin ligase enzyme BirA, which covalently attaches biotin to a specific lysine residue within the Avi-Tag sequence. The biotinylated protein can then be easily purified or detected using streptavidin-linked reagents, which bind specifically to biotin with high affinity. This makes the Avi-Tag a useful tool for studying protein localization, interaction, and function.
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