The FLAG tag (comprising the octapeptide sequence DYKDDDDK) is one of the most venerable and widely adopted epitope tags in molecular and cellular biology. A defining characteristic of the FLAG tag is its exceptional hydrophilicity, which typically exposes the peptide on the surface of the fusion protein, thereby maximizing its accessibility to cognate antibodies. Furthermore, its minimal size (approximately 1 kDa) ensures that it rarely interferes with the natural folding, stability, post-translational modifications, or native biological functions of the target recombinant protein.
The Mouse Anti-FLAG-Tag Monoclonal Antibody (CSB-MA000180, IgG isotype) is meticulously generated to provide high-affinity, monospecific recognition of this classic epitope sequence. Produced via robust hybridoma technology using a synthetic peptide as the immunogen, this antibody guarantees exceptional specificity and lot-to-lot consistency. Notably, the FLAG sequence contains a unique enterokinase cleavage site (DDDDK), allowing researchers not only to track and purify the protein but also to efficiently remove the tag if a native, untagged protein is required for downstream structural or functional studies.
Validated for both ELISA and Western Blot (WB) applications, the antibody exhibits excellent sensitivity and a high binding affinity. As vividly demonstrated in the Western Blot analysis, the antibody successfully detects the target recombinant protein at impressively high working dilutions of 1:5,000 (Lane 1) and 1:10,000 (Lane 2). The distinct, crisp bands observed on the membrane at both concentrations—with no visible signs of protein degradation, smearing, or non-specific background interference—underscore the antibody's excellent immunoreactivity and superior signal-to-noise ratio. The ability to maintain a strong detection signal even at a 1:10,000 dilution highlights its remarkable titer, making it an exceptionally cost-effective and powerful reagent for routine laboratory use.
This antibody is an indispensable tool for a broad spectrum of biological research. It serves as a critical reagent for verifying successful FLAG fusion protein expression across diverse systems (from bacterial to mammalian), accurately assessing protein molecular weight, and providing a reliable handle for downstream advanced biochemical assays, including affinity purification, co-immunoprecipitation (Co-IP) to map protein-protein interactions, and immunofluorescence microscopy for intracellular localization. With its proven performance and versatility, the FLAG-Tag Monoclonal Antibody ensures dependable, high-quality results to support research in protein engineering, signal transduction pathways, and functional genomics.
