His Monoclonal Antibody

The Mouse Anti-His-Tag Monoclonal Antibody (CSB-MA000011M1m, IgG1 isotype) is generated by immunizing mice with a diverse mixture of various His-tagged proteins. This complex antigen blend ensures the antibody recognizes the core 6xHis motif with high specificity across a broad range of fusion partners and experimental contexts. Following immunization, the hybridoma cell line is established, and the monoclonal antibody is subsequently purified via Protein G affinity chromatography to a high degree of homogeneity (>95%), guaranteeing exceptional lot-to-lot consistency and eliminating non-specific Fc region interactions.

Demonstrating remarkable versatility, this antibody has been stringently validated for Enzyme-Linked Immunosorbent Assay (ELISA), Western Blotting (WB), and Immunoprecipitation (IP). In Western Blot analyses, the antibody exhibits outstanding sensitivity, capable of detecting as little as 200ng of recombinant target protein at working dilutions spanning from an aggressive 1:8,000 down to a highly economical 1:256,000. The observed bands align perfectly with the predicted molecular weights, with only minor shifts in the apparent size attributable to expected glycosylation or post-translational modifications.

Furthermore, its efficacy in Immunoprecipitation underscores its robust binding avidity. The antibody successfully enriches His-tagged proteins from 293 cell lysates, effectively discriminating target antigens from a control IgG sample. This comprehensive validation across multiple platforms highlights its reliability as a cornerstone reagent for verifying recombinant protein expression, purifying native protein complexes, and conducting high-sensitivity quantitative assays in structural and functional genomics research.