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Western Blot
Positive WB detected in: 200ng recombinant protein with His tag His antibody at 1:8w, 1:16w, 1:32w, 1:64w, 1:128w, 1:256w
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 43.4 KDa
Observed band size: 43.4 KDa
Exposure time：5min
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Western Blot
Positive WB detected in: 200ng recombinant protein with His tag His antibody at 1:2w, 1:4w, 1:8w, 1:16w, 1:32w, 1:64w
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size:62.5 KDa
Observed band size: 66 KDa
Exposure time：5min
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Western Blot
Positive WB detected in:200ng of four Recombinant protein with his tag All lanes: His antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 43.4, 62.5,56,80.6 KDa
Observed band size:43.4, 66, 57 ,90 KDa
Exposure time：5min
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Immunoprecipitating His in 293 whole cell lysate transfected with His
Lane 1: control IgG instead of 2F4B5 in 293 whole cell lysate transfected with His. Lane 2: 2F4B5 antibody(2µg) + 293 whole cell lysate transfected with His (500µg)
Lane 3: 293 whole cell lysate transfected with His (5µg)
For western blotting, the blot was detected with 2F4B5 antibody at 1:10000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000
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