His Monoclonal Antibody

Datasheet

Code	CSB-MA000011M1m
Size	US$120
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Image	Western Blot Positive WB detected in: 200ng recombinant protein with His tag His antibody at 1:8w, 1:16w, 1:32w, 1:64w, 1:128w, 1:256w Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 43.4 KDa Observed band size: 43.4 KDa Exposure time：5min Western Blot Positive WB detected in: 200ng recombinant protein with His tag His antibody at 1:2w, 1:4w, 1:8w, 1:16w, 1:32w, 1:64w Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size:62.5 KDa Observed band size: 66 KDa Exposure time：5min Western Blot Positive WB detected in:200ng of four Recombinant protein with his tag All lanes: His antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 43.4, 62.5,56,80.6 KDa Observed band size:43.4, 66, 57 ,90 KDa Exposure time：5min Immunoprecipitating His in 293 whole cell lysate transfected with His Lane 1: control IgG instead of 2F4B5 in 293 whole cell lysate transfected with His. Lane 2: 2F4B5 antibody(2µg) + 293 whole cell lysate transfected with His (500µg) Lane 3: 293 whole cell lysate transfected with His (5µg) For western blotting, the blot was detected with 2F4B5 antibody at 1:10000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000
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Product Details
Customer Reviews and Q&A

Product Details

Alternative Names

6*His, 6*His, His Tag, 6histag, 6*His, HHHHHH, HIS, His Tag

Raised in

Mouse

Species Reactivity

All

Immunogen

The immunogen is a mixture of various His-tagged proteins

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

IgG1

Clone No.

2F4B5

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:640000
IP	2µl-5µl

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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