GAPDH Monoclonal Antibody

Datasheet
Code CSB-MA000071M0m
See More Details Free Antibody trial simple
Size US$200Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • Western Blot
    Positive WB detected in: 15μg hela whole cell lysate
    GAPDH antibody at 1:10W, 1:20W, 1:40W, 1:80W, 1:160W
    Secondary
    Goat polyclonal to mouse IgG at 1/50000 dilution
    Predicted band size: 36 KDa
    Observed band size: 36 KDa
    Exposure time: 5min

  • Western Blot
    Positive WB detected in: Hela whole cell lysate at 10μg, 5μg, 2.5μg, 1.25μg, 0.625μg, 0.3125μg
    All lanes: GAPDH antibody at 1:5000
    Secondary
    Goat polyclonal to mouse IgG at 1/50000 dilution
    Predicted band size: 36 KDa
    Observed band size: 36 KDa
    Exposure time: 5min

  • Western Blot
    Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate
    All lanes: GAPDH antibody at 1:2000
    Secondary
    Goat polyclonal to mouse IgG at 1/50000 dilution
    Predicted band size: 36 KDa
    Observed band size: 36 KDa
    Exposure time: 30s

  • IHC image of CSB-MA000071M0m diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-MA000071M0m diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-MA000071M0m diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of Hela cells with CSB-MA000071M0m at 1:220, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence staining of HepG2 cells with CSB-MA000071M0m at 1:220, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunoprecipitating GAPDH in Hela whole cell lysate
    Lane 1: Mouse control IgG instead of CSB-MA000071M0m in Hela whole cell lysate.
    Lane 2: CSB-MA000071M0m (3μl) + Hela whole cell lysate (500μg)
    Lane 3: Hela whole cell lysate (20μg)
    For western blotting, the blot was detected with CSB-MA000071M0m at 1:5000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000

  • Overlay histogram showing Hela cells stained with CSB-MA000071M0m (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1:200/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1:200/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

  • Overlay histogram showing Jurkat cells stained with CSB-MA000071M0m (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1:200/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1:200/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

Product Details

Alternative Names GAPDH; G3PD; GAPD; MGC88685
Raised in Mouse
Species Reactivity Human,Rat,Rabbit
Immunogen Recombinant Human GAPDH protein
Conjugate Non-conjugated
Clonality Monoclonal
Isotype IgG1
Clone No. 14C2F11
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC, IP, IF, FC
Recommended Dilution
Application Recommended Dilution
WB 1:2000-1:10000
IHC 1:50-1:500
IF 1:50-1:200
IP 1:2000-1:5000
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunoprecipitation (IP) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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Citations

Emerin suppresses Notch signaling by restricting the Notch intracellular domain to the nuclear membrane. B Lee.et al,Biochimica et biophysica acta-molecular cell research,2017

Applications: WB
Antibody dilution factor: 1: 5000
Review: HeLa cells were treated with si BAF #2 or si Control for 72 h. Cells were fractionated and subjected to western blot analyses (upper panel). Fractionation was verified by using Lamin A/C antibody (nuclear fraction) or GAPDH antibody (cytosolic fraction). The intensity of the cytosolic emerin was analyzed using ImageJ software (lower panel).
PMID: 27865926

A fluorescence method for determination of glucose transport by intestinal BBMV of common carp. Yang LP.et al,Anal Biochem,2017

Application: Immunoblot analyses
Review: Immunoblot analyses show the presence of SGLT-1 on BBMV of common carp.
PMID: 28847591

Q&A and Customer Reviews

 Customer Reviews
  • Tested application: WB
    Test sample: Culture cell (L-02)
    Sample volume: 15μg
    Primary antibody dilution ratio: 1:50,000
    Review: 1:5w requires a long exposure time and is noisy. It is expected that the effect of using 1:5k should be better. Overall, the WB effect is satisfactory. It is hoped that other label antibodies with high dilution ratio can be offered for trial.
  • Tested application: WB
    Test sample: Human ovarian granulosa cell carcinoma
    Sample volume: 12μg, 10μg, 8μg
    Primary antibody dilution ratio: 1:1200
    Review: Experiment success, antibody is usable!
  • Tested application: WB
    Test sample: KELLY
    Sample volume: 30μg
    Primary antibody dilution ratio: 1:1000
    Review: GAPDH has good results in this experiment, with correct size and single band.
  • Tested application: WB
    Test sample: 293T Cell
    Sample volume: 20μL
    Primary antibody dilution ratio: 1:2000
    Review: The protein is normal with clear bands, meeting the requirements of the experiment, with correct size and single bands.
  • Tested application: WB
    Test sample: Human ovarian granulosa cell carcinoma
    Sample volume: 10μg
    Primary antibody dilution ratio: 1:1000
    Review: The experiment was successful, and the antibody was successfully detected on human cells, which meets the experimental requirements and can be purchased and used in the future.
  • Tested application: WB
    Test sample: Rat kidney tissue
    Sample volume: 50μg
    Primary antibody dilution ratio: 1:5000
    Review: Good antibody, good titer.
  • Tested application: WB
    Test sample: Mouse
    Primary antibody dilution ratio: 1:5000
    Review: Antibody specificity is very good.
  • Tested application: WB
    Test sample: Human 231 cell
    Primary antibody dilution ratio: 1:2000
    Review: The destination band is correct and single.
  • Applications: WB
    Review: Immunoblot analyses show the presence of SGLT-1 on BBMV of common carp. Left lane: SGLT-1: BBMV. Right lane: GAPDH.
    PMID: 28847591
  • Applications: WB
    Review: HeLa cells were treated with si BAF #2 or si Control for 72 h. Cells were fractionated and subjected to western blot analyses (upper panel). Fractionation was verified by using Lamin A/C antibody (nuclear fraction) or GAPDH antibody (cytosolic fraction). The intensity of the cytosolic emerin was analyzed using ImageJ software (lower panel).
    PMID: 27865926

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