GAPDH Monoclonal Antibody

Datasheet

Code	CSB-MA000071M0m
Size	US$120
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Image	Western Blot Positive WB detected in: 15μg hela whole cell lysate GAPDH antibody at 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 36 KDa Observed band size: 36 KDa Exposure time: 5min Western Blot Positive WB detected in: Hela whole cell lysate at 10μg, 5μg, 2.5μg, 1.25μg, 0.625μg, 0.3125μg All lanes: GAPDH antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 36 KDa Observed band size: 36 KDa Exposure time: 5min Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate All lanes: GAPDH antibody at 1:2000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 36 KDa Observed band size: 36 KDa Exposure time: 30s Western Blot Positive WB detected in: U87 whole cell lysate, PC3 whole cell lysate, 293 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, A375 whole cell lysate, MG-63 whole cell lysate All lanes GAPDH antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 36 KDa Observed band size: 36 KDa Exposure time: 30s Western Blot Positive WB detected in: Rat heart tissue, Rat kidney tissue, Rat skeletal muscle tissue, Rat liver tissue, Rat brain tissue tissue, Rat spleen tissue All lanes GAPDH antibody at 1:1000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 36 KDa Observed band size: 36 KDa Exposure time: 1min Western Blot Positive WB detected in: Rabbit heart tissue, Rabbit liver tissue,Rabbit spleen tissue, Rabbit lung tissue, Rabbit kidney tissue, Rabbit small intestine tissue, Rabbit skeletal muscle tissue All lanes GAPDH antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 36 KDa Observed band size: 36 KDa Exposure time: 5min IHC image of CSB-MA000071M0m diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-MA000071M0m diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-MA000071M0m diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-MA000071M0m at 1:220, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Immunofluorescence staining of HepG2 cells with CSB-MA000071M0m at 1:220, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Immunoprecipitating GAPDH in Hela whole cell lysate Lane 1: Mouse control IgG instead of CSB-MA000071M0m in Hela whole cell lysate. Lane 2: CSB-MA000071M0m (1μl) + Hela whole cell lysate (500μg) Lane 3: Hela whole cell lysate (20μg) For western blotting, the blot was detected with CSB-MA000071M0m at 1:5000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000 Overlay histogram showing Hela cells stained with CSB-MA000071M0m (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1:200/110⁶cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1:200/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed. Overlay histogram showing Jurkat cells stained with CSB-MA000071M0m (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1:200/110⁶cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1:200/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed. 1.Exosomes extracted from HEPG2 cells 2. Exosomes extracted from PC-3 cells 3. Exosomes extracted from Hela cells 4. Exosomes extracted from U87 cells 5. Hela cell Lysate 1.Exosomes extracted from MG63 cells 2. Exosomes extracted from Ntera-2 cells 3. MG63 cell Lysate 1. Exosomes extracted from Raji cells 2. Exosomes extracted from U251 cells 3. Raji cell Lysate
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Product Details
Citations
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Target Background

Product Details

Full Product Name

Mouse anti-Homo sapiens (Human) GAPDH Monoclonal antibody

Uniprot No.

P04406

Target Names

GAPDH

Alternative Names

GAPDH; G3PD; GAPD; MGC88685

Raised in

Mouse

Species Reactivity

Human, Rat, Rabbit

Immunogen

Recombinant Human GAPDH protein (3-335AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

IgG1

Clone No.

14C2F11

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IP, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:5000-1:1600000
IHC	1:50-1:500
IF	1:50-1:200
IP	1µl-2µl
FC	1:100-1:300

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunoprecipitation (IP) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The GAPDH Monoclonal Antibody is a specific antibody that targets GAPDH. The GAPDH antibody is an internal reference antibody that functions as a loading control to ensure equal protein loading and accurate quantification of protein expression levels in different samples. This antibody can detect GAPDH in human, mouse, and rabbit species.

The immunogen used to generate this GAPDH antibody is the 2-335 amino acid region of recombinant Human GAPDH protein. The GAPDH Monoclonal Antibody is raised in mouse and belongs to the IgG1 isotype. It is purified using Protein G and reaches a purity level of greater than 95%.

The GAPDH Monoclonal Antibody is available in liquid form and has been tested in various applications, including ELISA, WB, IHC, IP, and IF. These applications make the antibody a versatile tool for the detection and analysis of GAPDH in different contexts.

Moreover, the GAPDH Monoclonal Antibody has been cited in a paper by H Miao, et al. in 2022, which highlights its utility in scientific research. The use of this validated antibody in research increases the reliability of the results and ensures reproducibility.

Citations

CXCL1 confers a survival advantage in Kaposi's sarcoma‐associated herpesvirus‐infected human endothelial cells through STAT3 phosphorylation MJ Lee,Journal of medical virology,2023
Concurrent EPA and DHA Supplementation Impairs Brown Adipogenesis of C2C12 Cells S Ghnaimawi,Frontiers in Genetics,2020
Quadrella incana (Capparaceae) Leaf Extract Enhances Proliferation and Maintenance of Neural Stem/Progenitor Cells through Upregulating Glycolytic Flux and Redox Potential M Kang,Oxid Med Cell Longev,2020
Emerin suppresses Notch signaling by restricting the Notch intracellular domain to the nuclear membrane B Lee.et al,Biochimica et biophysica acta-molecular cell research,2017
A fluorescence method for determination of glucose transport by intestinal BBMV of common carp Yang LP.et al,Anal Biochem,2017

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