Myc tag Monoclonal Antibody

The Myc tag (EQKLISEEDL) is a widely adopted 10-amino-acid peptide sequence used extensively in recombinant protein engineering. As a versatile epitope tag, it facilitates the detection, purification, and tracking of heterologous proteins within complex cellular environments, making it an indispensable tool for molecular biology and biochemistry research.

This mouse Myc tag monoclonal antibody (IgG1 isotype) is generated against a synthetic peptide corresponding to the Myc tag sequence, conjugated to Keyhole Limpet Hemocyanin (KLH), and subsequently purified via Protein G affinity chromatography to ensure high purity (>95%). It demonstrates robust immunoreactivity and exceptional specificity across multiple assay platforms.

Rigorous in-house validation confirms its efficacy in Western Blot (WB), Enzyme-Linked Immunosorbent Assay (ELISA), Immunofluorescence (IF), and Immunoprecipitation (IP) applications. In WB experiments, the antibody detects recombinant Myc-tagged fusion protein with high sensitivity, clearly identifying concentrations as low as 1.5625 ng, with the observed band size precisely aligning with the predicted 50 kD molecular weight. Furthermore, its performance in IF and IP workflows validates its capability to specifically recognize endogenous and transiently expressed Myc-tagged targets in fixed cells and complex lysates, respectively, making it an excellent reagent for studying protein-protein interactions and subcellular localization.

As a rigorously validated immunological tool, this antibody serves as a critical reagent for confirming recombinant protein expression, facilitating affinity purification, and supporting a wide range of downstream biochemical analyses. Cited in 2 peer-reviewed scientific publications, this CUSABIO monoclonal antibody is backed by comprehensive technical support, ensuring dependable and reproducible results to accelerate your cutting-edge research.