Myc tag Monoclonal Antibody

Datasheet

Code	CSB-MA000041M0m
Size	US$120
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Image	WB: Mouse anti Myc-tagged fusion protein Monoclonal antibody at 1.6μg/ml Lane 1: Recombinant Myc-tagged fusion protein at 50ng Lane 2: Recombinant Myc-tagged fusion protein at 25ng Lane 3: Recombinant Myc-tagged fusion protein at 12.25ng Lane 4: Recombinant Myc-tagged fusion protein at 6.25ng Lane 5: Recombinant Myc-tagged fusion protein at 3.125ng Lane 6: Recombinant Myc-tagged fusion protein at 1.5625ng Secondary Goat polyclonal to Mouse IgG at 1/50000 dilution Predicted band size: 50 kd Observed band size: 50 kd Immunofluorescence staining of transfected HEK293 cells with CSB-MA000041M0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L). Immunoprecipitating MYC-tag with transfected HEK293 Lane 1: Mouse control IgG (1µg) instead of CSB-MA000041M0m in transfected HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-MA000041M0m (6µg) + transfected HEK293 whole cell lysate (1mg) Lane 3: Transfected HEK293 whole cell lysate (10µg)
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Product Details
Citations
Customer Reviews and Q&A

Product Details

Alternative Names

bHLHe39, c Myc, MRTL, MYC, Myc proto oncogene protein, MYC tag, Proto oncogene c Myc, Transcription factor p64

Raised in

Mouse

Species Reactivity

N/A

Immunogen

EQKLISEEDL (Myc) synthetic peptide conjugate to KLH

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

IgG1

Clone No.

5F92F9

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IF, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IF	1:100-1:300
IP	1:1000-1:1500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Myc tag monoclonal antibody CSB-MA000041M0m was produced in the mouse immunized by using the EQKLISEEDL (Myc) synthetic peptide conjugate to KLH as the immunogen. The target peptide Myc is a popular short peptide tag (EQKLISEEDL) derived from the c-myc gene product and recognized by numerous commercial antibodies as a tag. This tag can be added to the C- or N-terminus by recombinant DNA technology and can be used for affinity chromatography and for isolating protein complexes with multiple subunits.
This Myc tag monoclonal antibody was tested in the ELISA, WB, IF and IP. The non-conjugated IgG1 got purified by protein G and reached up to 95% in purity. It can be used for detection and expression of Myc tag fusion expression proteins, intracellular localization, and purification, qualitative or quantitative detection of Myc fusion-expressed proteins and the like. It doesn’t have species restricted.

Citations

Borealin-nucleosome interaction secures chromosome association of the chromosomal passenger complex Abad M A, et al,The Journal of Cell Biology,2019
The double-edged sword of endoplasmic reticulum stress in uremic sarcopenia through myogenesis perturbation Jheng JR.et al,J Cachexia Sarcopenia Muscle,2018

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 4 reviews

Submit a Review here

Applications : Western Blot (WB)

Sample type: Cells

Sample dilution: 1:500

Review: Antibody specificity is very good.

By Anonymous

Applications : Western Blot (WB)

Sample type: HEK293 cell lysate

Sample dilution: 1:1000

Review: Myc antibody has good specificity.

By Anonymous

Applications : Immunoblotting

Sample dilution: 1:5000

Review: Since YFPN and YFPC are fused to Myc and HA tags, respectively, NbRbgA:YFPN and cpRPL:YFPC proteins were detected by immunoblotting using anti-Myc and anti-HA antibodies, respectively.

By Anonymous

Applications : WB

Sample type: Mouse HeLa Kyoto cells

Sample dilution: 1:1000

Review: Analysis of the expression levels of the Myc-Borealin constructs used in Fig. 4 (B and C).

By Anonymous

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