Myc-Tag Monoclonal Antibody

Code CSB-MA000157
Size US$119
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Image
  • IP antibody use: 3µg Flag Mouse IgG1 per ml Lysate, WB 1:5000
    Lane 1: untransfected 293 cell lysate
    Lane 2: transfected 293 cell lysate with C-Myc-tag fusion protein
    Lane 3: IP (transfected 293 + normal Mouse IgG + Protein G agarose)
    Lane 4: IP (transfected 293 + anti- C-Myc mAb + Protein G agarose)
    Lane 5: IP (transfected 293 + Protein G)
    Lane 6: Recombinant protein (E.coli)

  • IF analysis of 293 cells transfected with a Myc-tag protein,1:2000 dilution (blue DAPI, red anti-Myc)

  • 1µg Myc fusion protein + Primary antibody dilution at 1) 1:5000 2) 1:10000

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Product Details

Target Names
Myc-Tag
Raised in
Mouse
Species Reactivity
N/A
Immunogen
Synthetic Peptide
Conjugate
Non-conjugated
Isotype
IgG
Clone No.
25D6
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Form
Liquid
Tested Applications
ELISA, WB, IF, IP
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IF 1:50-1:200
IP 1:200-1:2000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

The Myc epitope tag is a widely adopted peptide marker for recombinant protein detection, purification, and subcellular localization analysis in molecular and cellular biology research. This Myc-Tag monoclonal antibody (CSB-MA000157) is generated in mouse with IgG isotype, raised against a synthetic peptide immunogen, and supplied as a non-conjugated liquid formulation for MYC-tagged protein studies. It exhibits exceptional specificity for Myc-tagged fusion proteins, low off-target background, and flexible working dilution windows, delivering cost-effective, reproducible performance for both routine bench assays and high-throughput experimental pipelines. WB analysis yields sharp, distinct bands when probing 1 μg of Myc fusion protein at primary antibody dilutions of 1:5000 and 1:10000; for immunofluorescence, bright and well-defined fluorescent signals are observed in Myc-transfected 293 cells at a 1:2000 working dilution; immunoprecipitation assays further demonstrate efficient target enrichment from transfected 293 cell lysates with minimal non-specific binding in negative controls, matching the optimized application-specific dilution ranges. Fully validated for ELISA, WB, IF and IP, this tag antibody provides a robust solution for Myc-tagged recombinant protein expression confirmation, subcellular localization mapping and affinity purification in protein engineering, cell biology and biopharmaceutical research.

Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China
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