| Code | CSB-MA000157 |
| Size | US$119 |
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| Application | Recommended Dilution |
|---|---|
| WB | 1:500-1:5000 |
| IF | 1:50-1:200 |
| IP | 1:200-1:2000 |
The Myc epitope tag is a widely adopted peptide marker for recombinant protein detection, purification, and subcellular localization analysis in molecular and cellular biology research. This Myc-Tag monoclonal antibody (CSB-MA000157) is generated in mouse with IgG isotype, raised against a synthetic peptide immunogen, and supplied as a non-conjugated liquid formulation for MYC-tagged protein studies. It exhibits exceptional specificity for Myc-tagged fusion proteins, low off-target background, and flexible working dilution windows, delivering cost-effective, reproducible performance for both routine bench assays and high-throughput experimental pipelines. WB analysis yields sharp, distinct bands when probing 1 μg of Myc fusion protein at primary antibody dilutions of 1:5000 and 1:10000; for immunofluorescence, bright and well-defined fluorescent signals are observed in Myc-transfected 293 cells at a 1:2000 working dilution; immunoprecipitation assays further demonstrate efficient target enrichment from transfected 293 cell lysates with minimal non-specific binding in negative controls, matching the optimized application-specific dilution ranges. Fully validated for ELISA, WB, IF and IP, this tag antibody provides a robust solution for Myc-tagged recombinant protein expression confirmation, subcellular localization mapping and affinity purification in protein engineering, cell biology and biopharmaceutical research.
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