Myc-Tag Monoclonal Antibody

The Myc tag, originating from the human c-Myc proto-oncogene, consists of a 10-amino acid peptide (EQKLISEEDL). Its petite size and hydrophilic nature make it an exceptionally versatile tool for molecular biology, rarely disrupting the native conformation or function of the attached recombinant protein. This renders it ideal for a diverse spectrum of applications, from bacterial expression to advanced mammalian cell studies and intricate protein interaction mapping.

The Mouse Anti-c-Myc Monoclonal Antibody (CSB-MA000179, IgG isotype) is expertly generated to deliver high-affinity, monospecific binding to this specific epitope. Utilizing state-of-the-art hybridoma technology, this reagent is produced and subsequently subjected to rigorous affinity-chromatography purification using its specific immunogen directly from mouse ascites. This meticulous process guarantees exceptional specificity, significant batch-to-batch uniformity, and minimal cross-reactivity with host cellular components, ensuring reliable experimental outcomes.

Validated for both ELISA and Western Blot (WB) applications, the antibody showcases remarkable sensitivity and superior binding capacity. As evidenced in the Western Blot analysis, the antibody successfully identifies the target recombinant protein even when diluted to extremely high working concentrations of 1:50,000 (Lane 1) and 1:100,000 (Lane 2). The presence of sharp, intense bands at these substantial dilutions, devoid of detectable background noise or protein degradation artifacts, unequivocally demonstrates the antibody's outstanding immunoreactivity and impressive signal-to-noise ratio. Such high titers significantly reduce per-test consumption, establishing it as an extraordinarily economical and potent reagent for demanding laboratory environments.

This antibody stands as an essential asset for researchers engaged in molecular and cellular biology. It acts as a definitive tool for confirming successful c-Myc fusion protein expression, precisely determining protein molecular weights, and serving as a robust handle for a multitude of downstream biochemical endeavors. These include, but are not limited to, affinity purification, co-immunoprecipitation (Co-IP) for interaction studies, and immunofluorescence for intracellular localization. With its proven efficacy and adaptability, the c-Myc Monoclonal Antibody ensures consistently high-quality, dependable results, effectively supporting pioneering research in protein engineering, gene function elucidation, and structural biology.