V5-Tag Monoclonal Antibody

Datasheet

Code	CSB-MA000160
Size	US$119
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Image	IP antibody use: 5µg V5 Mouse IgG1 per ml Lysate, WB 1: 5000 Lane 1: untransfected 293 cell lysate Lane 2: transfected 293 cell lysate with V5-tag fusion protein Lane 3: IP (untransfted 293 + anti-V5 mAb + Protein G agarose) Lane 4: IP (transfected 293 + normal Mouse IgG + Protein G agarose) Lane 5: IP (transfected 293 + anti-V5 mAb + Protein G agarose) IF analysis of 293 cells transfected with a V5-tag protein,1:2000 dilution (blue DAPI, red anti-V5) 1µg V5 fusion protein + Primary antibody dilution at 1) 1:5000 2) 1:10000
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Product Details
Customer Reviews and Q&A

Product Details

Raised in

Mouse

Species Reactivity

N/A

Immunogen

Synthetic Peptide

Conjugate

Non-conjugated

Isotype

IgG

Clone No.

18G5

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IF, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IF	1:200-1:2000
IP	1:200-1:2000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The V5 epitope tag is a versatile peptide marker extensively used for detecting, purifying, and localizing recombinant fusion proteins in molecular and cellular biology research. This V5-Tag monoclonal antibody (CSB-MA000160) is produced in mouse with an IgG isotype, raised against a synthetic V5 peptide, and supplied as a non-conjugated liquid format for tag-targeted protein analysis. It exhibits high specificity and affinity for V5-tagged recombinant proteins, minimal non-specific background, and flexible working dilution ranges, delivering reliable and cost-efficient performance across diverse experimental setups. WB validation shows clear, specific bands against 1 μg of V5 fusion protein at dilutions of 1:5000 and 1:10000; immunofluorescence staining yields bright, well-defined signals in V5-transfected 293 cells at 1:2000 dilution; immunoprecipitation assays demonstrate efficient target enrichment from transfected 293 cell lysate with negligible background in negative controls, matching the optimized recommended dilution ranges for each application. Fully validated for ELISA, WB, IF, and IP, this antibody provides a dependable solution for the expression verification, subcellular localization analysis, and affinity purification of V5-tagged recombinant proteins in protein engineering, cell biology, and biopharmaceutical research.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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