V5-Tag Monoclonal Antibody

Derived from the P and V proteins of the simian virus 5 (SV5), the V5 epitope tag is a highly practical 14-amino acid peptide sequence (GKPIPNPLLGLDST). Valued for its compact footprint, the V5 tag is frequently appended to the N- or C-terminus of target proteins, serving as a universal handle for detection and affinity purification. Its small size ensures that it generally does not impede the natural folding or biological activity of the recombinant molecule.

The Mouse Anti-V5-Tag Monoclonal Antibody (CSB-MA000177) is crafted to deliver precise and monospecific recognition of this viral epitope. Produced via rigorous hybridoma technology, this IgG-class reagent leverages a synthetic peptide immunogen to ensure exceptional specificity and lot-to-lot uniformity across experimental batches.

Validated for both ELISA and Western Blot applications, this antibody exhibits remarkable sensitivity. As illustrated in the accompanying Western Blot analysis, the antibody successfully identifies the V5-tagged recombinant protein (Q31) at impressively high working dilutions of 1:5,000 and 1:10,000. The distinct, dark bands observed at both concentrations, conspicuously free of background interference or protein degradation artifacts, underscore the antibody's superior immunoreactivity and high signal-to-noise ratio. Such high titers position this reagent as a remarkably cost-effective solution for high-throughput and demanding laboratory environments.

This monoclonal antibody serves as an indispensable tool for verifying recombinant protein expression, mapping protein interactions, and conducting affinity purification. With its proven performance, the V5-Tag Monoclonal Antibody ensures dependable, high-quality results, robustly supporting research in molecular biology and protein engineering.