Recombinant Arabidopsis thaliana Acyl-CoA-binding domain-containing protein 2 (ACBP2)

Code CSB-CF879837DOA
MSDS
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Source in vitro E.coli expression system
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Product Details

Target Names
ACBP2
Uniprot No.
Alternative Names
ACBP2; At4g27780; T27E11.20; Acyl-CoA-binding domain-containing protein 2; Acyl-CoA binding protein 2
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-354
Target Protein Sequence
MGDWAQLAQSVILGLIFSYLLAKLISIVVTFKEDNLSLTRHPEESQLEIKPEGVDSRRLD SSCGGFGGEADSLVAEQGSSRSDSVAGDDSEEDDDWEGVESTELDEAFSAATLFVTTAAA DRLSQKVPSDVQQQLYGLYKIATEGPCTAPQPSALKMTARAKWQAWQKLGAMPPEEAMEK YIEIVTQLYPTWLDGGVKAGSRGGDDAASNSRGTMGPVFSSLVYDEESENELKIDAIHGF AREGEVENLLKSIESGIPVNARDSEGRTPLHWAIDRGHLNIAKVLVDKNADVNAKDNEGQ TPLHYAVVCDREAIAEFLVKQNANTAAKDEDGNSPLDLCESDWPWIRDSAKQAD
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Binds medium- and long-chain acyl-CoA esters with very high affinity. Can interact in vitro with palmitoyl-CoA, but not with oleoyl-CoA. Binds to lead ions (Pb). May function as an intracellular carrier of acyl-CoA esters. Required for proper phospholipid and, to a lower extent, galactolipid composition.
Gene References into Functions
  1. ACBP2, which shows high (76.9%) conservation in amino acid homology to ACBP1, did not appear to be involved in the freezing response. PMID: 20404541
  2. These observations support a positive role for ACBP2 in promoting abscissic acid signalling in germination, seedling development and the drought response. PMID: 22788984
  3. ACBP2 can mediate tolerance to Cd(II)-induced oxidative stress by interacting with two protein partners, AtFP6 and lysoPL2. PMID: 20657176
  4. ACBP2 can bind lysoPL2 to promote the degradation of lysophosphatidylcholine in response to Cd-induced oxidative stress. PMID: 20345607
  5. ACBP1 and ACBP2 are essential in lipid transfer during early embryogenesis in Arabidopsis. PMID: 20345632
  6. observed to interact with AtFP6...an ACBP2 derivative lacking ankyrin repeats did not interact with AtFP6, indicating that the ankyrin repeats mediate interaction; plants overexpressing ACBP2 or AtFP6 were more tolerant to Cd(II) than wild-type plants. PMID: 18823312

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Subcellular Location
Cell membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein. Peroxisome membrane; Single-pass membrane protein.
Protein Families
ACBP family
Tissue Specificity
Mostly expressed in roots and flowers, and, to a lower extent, in stems, pods and leaves (at protein level).
Database Links

KEGG: ath:AT4G27780

STRING: 3702.AT4G27780.1

UniGene: At.25382

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