Recombinant Drosophila melanogaster Calcium release-activated calcium channel protein 1 (olf186-F)

Code CSB-CF890637DLU
MSDS
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Source in vitro E.coli expression system
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Product Details

Target Names
olf186-F
Uniprot No.
Alternative Names
Orai; CRACM1; olf186-F; CG11430; Calcium release-activated calcium channel protein 1; Protein orai
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-351
Target Protein Sequence
MSVWTTANNSGLETPTKSPITSSVPRAARSSAVITTGNHQQHHFQHVVAAAVAAATSVAT GHQFQQQFPLHAHPHPQHHSNSPTGSGSNSNNSAGFQRTSISNSLLQFPPPPPPSSQNQA KPRGHHRTASSSMSQSGEDLHSPTYLSWRKLQLSRAKLKASSKTSALLSGFAMVAMVEVQ LDHDTNVPPGMLIAFAICTTLLVAVHMLALMISTCILPNIETVCNLHSISLVHESPHERL HWYIETAWAFSTLLGLILFLLEIAILCWVKFYDLSPPAAWSACVVLIPVMIIFMAFAIHF YRSLVSHKYEVTVSGIRELEMLKEQMEQDHLEHHNNIRNNGMNYGASGDIV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Ca(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx following depletion of intracellular Ca(2+) stores. Regulates transcription factor NFAT nuclear import.
Gene References into Functions
  1. Ca2+ uptake in neurons with reduced SEPT7 requires Orai. PMID: 27225060
  2. These studies suggest that modulation of SOCE could serve as a novel mechanism for restoring dopamine levels in dopaminergic neurons. PMID: 26446229
  3. The crystal structure of Orai from Drosophila melanogaster, determined at 3.35 angstrom resolution, reveals that the calcium channel is composed of a hexameric assembly of Orai subunits arranged around a central ion pore. PMID: 23180775
  4. Orai1 is an essential component or regulator of the CRAC channel complex PMID: 16582901
  5. results demonstrate that the protein CRACM1 encoded by olf186-F is essential for store-operated Ca2+ influx via Ca2+ release-activated Ca2+ (CRAC) channels PMID: 16645049
  6. results indicate that Orai itself forms the Ca2+-selectivity filter of the CRAC channel PMID: 16921385
  7. The human ortholog of drosophila olf186-F interacts with STIM1 to regulate the amplitude of store-operated calcium entry. PMID: 16733527

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Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
Orai family
Database Links

KEGG: dme:Dmel_CG11430

STRING: 7227.FBpp0113106

UniGene: Dm.7627

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