Recombinant Drosophila melanogaster G-protein coupled receptor Mth (mth)

Code CSB-CF529762DLU
MSDS
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Source in vitro E.coli expression system
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Product Details

Target Names
mth
Uniprot No.
Alternative Names
mth; CG6936; G-protein coupled receptor Mth; Protein methuselah
Species
Drosophila melanogaster (Fruit fly)
Expression Region
25-514
Target Protein Sequence
DILECDYFDTVDISAAQKLQNGSYLFEGLLVPAILTGEYDFRILPDDSKQKVARHIRGCV CKLKPCVRFCCPHDHIMDNGVCYDNMSDEELAELDPFLNVTLDDGSVSRRHFKNELIVQW DLPMPCDGMFYLDNREEQDKYTLFENGTFFRHFDRVTLRKREYCLQHLTFADGNATSIRI APHNCLIVPSITGQTVVMISSLICMVLTIAVYLFVKKLQNLHGKCFICYMVCLFMGYLFL LLDLWQISISFCKPAGFLGYFFVMAAFFWLSVISLHLWNTFRGSSHKANRFLFEHRFLAY NTYAWGMAVVLTGITVLADNIVENQDWNPRVGHEGHCWIYTQAWSAMLYFYGPMVFLIAF NITMFILTAKRILGVKKDIQNFAHRQERKQKLNSDKQTYTFFLRLFIIMGLSWSLEIGSY FSQSNQTWANVFLVADYLNWSQGIIIFILFVLKRSTWRLLQESIRGEGEEVNNSEEEISL ENTTTRNVLL
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in biological aging and stress response. Essential for adult survival. Required in the presynaptic motor neuron to up-regulate neurotransmitter exocytosis at larval glutamatergic neuromuscular junctions (NMJs). Regulates a step associated with docking and clustering of vesicles at release sites. SP/Acp70A and sun are agonists that activate mth in vitro.
Gene References into Functions
  1. this study identifies Methuselah (Mth) as a secretin-incretin receptor subfamily member required in the insulin-producing cells for proper nutrient coupling. PMID: 27708106
  2. Methuselah gene mutation is associated with tolerance of dichlorvos exposure in Drosophila melanogaster. PMID: 25746179
  3. mth has a statistically significant advantage in maintaining continuous flight over control flies at age 10 days, but not during middle and late life. mth flies had lower levels of locomotor activity relative to controls at ages 50 and 60 days. PMID: 23913251
  4. While our data support a role for mth variation being under natural selection, we found no link to naturally occurring variation in lifespan and ageing in Australian populations of D. melanogaster. PMID: 23802551
  5. Identified two additional activators of Mth signaling: Drosophila Sex Peptide (SP) and a novel peptide (Serendipitous Peptide Activator of Mth, SPAM). PMID: 19672878
  6. where the life span is extended, there is an offsetting effect on reproductive output, suggesting that the methuselah mutation induces trade-off effects and is not a direct, mechanistic regulator of the aging process. PMID: 16677788
  7. Mth gene delays chronological aging but also enhances sensorimotor abilities critical to survival during early and middle, but not late life. PMID: 17534708
  8. allelic variation at mth contributes to observed differences in lifespan and correlated phenotypes in natural populations of Drosophila PMID: 18414670

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Subcellular Location
Cell membrane; Multi-pass membrane protein. Note=Plasma membrane of presynaptic terminals and innervating axons.
Protein Families
G-protein coupled receptor 2 family, Mth subfamily
Database Links

KEGG: dme:Dmel_CG6936

STRING: 7227.FBpp0072470

UniGene: Dm.4379

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