Recombinant Escherichia coli Outer membrane protein tolC (tolC)-VLPs

1. TolC, a potentially interesting pharmaceutical target can be addressed by small molecules, blocking the channel. Biophysical characterization of the binding processes will support future identification and optimisation of more potent TolC blockers in order to validate TolC as a pharmaceutical target. PMID: 28746830
2. The authors report the near-atomic resolution cryoEM structures of the Escherichia coli AcrAB-TolC multidrug efflux pump in resting and drug transport states, revealing a quaternary structural switch that allosterically couples and synchronizes initial ligand binding with channel opening. PMID: 28355133
3. A number of multi-drug efflux genes, particularly the central component TolC, show higher expression in antibiotic resistant Escherichia coli. PMID: 27105118
4. Data suggest that outer membrane channel TolC plays an important role in extrusion of isoprenol and other medium-chain alcohols (CmOHs). PMID: 26563610
5. Thus, tolC mutants grown in low iron conditions accumulate periplasmic enterobactin, which impairs bacterial morphology, possibly by sequestering iron and inhibiting an iron-dependent reaction involved in cell division or peptidoglycan synthesis. PMID: 24330203
6. Interactions between TolC and AcrAB are highly dynamic and only a small fraction of TolC is utilized to support efflux activities. PMID: 23331412
7. The TolC enhancement of extreme-acid survival includes Gad induction, but TolC-dependent growth rates below pH 6.5 do not involve Gad. PMID: 21541325
8. These results indirectly suggest that TolC and YiaT proteins may play some role(s) in multidrug resistance. PMID: 20639525
9. Inactivation of TolC triggers metabolic shutdown in E. coli cells grown in minimal glucose medium. PMID: 20545840
10. essential for enterobactin export across the outer membrane PMID: 16166532
11. The mechanism of tetracycline resistance was studied using expression studies in E. coli mutants that were defective in the tet(A) and the tolC proteins. PMID: 16687460
12. Dada show that a combination of sbmA and tolC mutations appears to have additive effect, resulting in almost complete suppression of the phenotypic expression of Tn10 tetracycline resistance. PMID: 18083810
13. Results describe two crystal structures of the Escherichia coli outer membrane protein TolC in its partially open state. PMID: 18406332
14. TolC-dependent efflux system is involved in the exclusion of porphyrins in E. coli. PMID: 18641137
15. The authors conclude that TolC plays an important role in L: -cysteine tolerance probably due to its export ability and that TolC overexpression is effective for L-cysteine production in E. coli. PMID: 18828007
16. Data that TolC is involved in the efflux of certain cellular metabolites that accumulate during growth and trigger the upregulation of MarA, SoxS, and Rob, which in turn upregulate tolC and help rid the bacteria of these metabolites. PMID: 19502391

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KEGG: ecj:JW5503

STRING: 316385.ECDH10B_3209