Recombinant Human Desmoglein-1(DSG1),partial

Code CSB-CF861938HU
Size US$1235Purchase it in Cusabio online store
(only available for customers from the US)
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-CF861938HU could indicate that this peptide derived from E.coli-expressed Homo sapiens(Human) DSG1.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-CF861938HU could indicate that this peptide derived from E.coli-expressed Homo sapiens(Human) DSG1.
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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names DSG1
Uniprot No. Q02413
Research Area Signal Transduction
Alternative Names Cadherin family member 4; CDHF4; Desmoglein-1; Desmosomal glycoprotein 1; DG1; DGI; DSG; DSG1; DSG1_HUMAN; EPKHE; EPKHIA; Pemphigus foliaceus antigen; PPKS1; SPPK1
Species Homo sapiens(Human)
Source in vitro E.coli expression system
Expression Region 50-548aa
Target Protein Sequence EWIKFAAACREGEDNSKRNPIAKIHSDCAANQQVTYRISGVGIDQPPYGIFVINQKTGEINITSIVDREVTPFFIIYCRALNSMGQDLERPLELRVRVLDINDNPPVFSMATFAGQIEENSNANTLVMILNATDADEPNNLNSKIAFKIIRQEPSDSPMFIINRNTGEIRTMNNFLDREQYGQYALAVRGSDRDGGADGMSAECECNIKILDVNDNIPYMEQSSYTIEIQENTLNSNLLEIRVIDLDEEFSANWMAVIFFISGNEGNWFEIEMNERTNVGILKVVKPLDYEAMQSLQLSIGVRNKAEFHHSIMSQYKLKASAISVTVLNVIEGPVFRPGSKTYVVTGNMGSNDKVGDFVATDLDTGRPSTTVRYVMGNNPADLLAVDSRTGKLTLKNKVTKEQYNMLGGKYQGTILSIDDNLQRTCTGTININIQSFGNDDRTNTEPNTKITTNTGRQESTSSTNYDTSTTSTDSSQVYSSEPGNGAKDLLSDNVHFGP
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 60.4kDa
Protein Length Extracellular Domain
Tag Info N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Background

Function
(From Uniprot)
Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
Gene References into Functions
  1. Here the authors identify a potential mechanism by which desmosomes assist the de-neddylating COP9 signalosome (CSN) in attenuating EGFR through an association between the Cops3 subunit of the CSN and desmosomal components, Desmoglein1 (Dsg1) and Desmoplakin (Dp), to promote epidermal differentiation. PMID: 28891468
  2. Compared with immunocompetent patients, kidney transplant recipients systemically treated with calcineurin inhibitors and mycophenolate mofetil exhibited significantly reduced epidermal expression of Dsg1, a regulator of keratinocyte differentiation and barrier function. PMID: 27302902
  3. Study identified DSG1 to be involved in the progressive intestinal injury associated with the development of Crohn's Disease complications via its effect on intestinal cell adhesion. PMID: 28490445
  4. The authors report one recurrent and five novel DSG1 mutations, causing varying patterns of PPK, highlighting the clinical heterogeneity arising from mutations in this gene. PMID: 27534273
  5. Authors report a new family of severe dermatitis, multiple allergies and metabolic wasting syndrome of Asian decent and expand the spectrum of mutations in the DSG1 gene. PMID: 27154412
  6. Case Report: striate palmoplantar keratoderma showing transgrediens in a patient with heterozygote nonsense mutations in DSG1 and SERPINB7. PMID: 27786350
  7. Genetic deletion of sdrD in S. aureus NCTC8325-4 through allelic replacement resulted in decreased bacterial adherence to Dsg1- expressing HaCaT cells in vitro. PMID: 26924733
  8. The results of this study describes the fourth family with a biallelic mutation in the DSG1 gene associated with SAM syndrome, and emphasizes that the presence of PPK and skin fragility with or without additional symptoms should alert clinicians to consider DSG1 deficiency. PMID: 26288349
  9. Desmoglein 1 mutation as cause of SAM syndrome PMID: 26169895
  10. Desmoglein-1 regulates esophageal epithelial barrier function and immune responses in eosinophilic esophagitis. PMID: 24220297
  11. Ectopic expression of Dsg1 in keratinocyte monolayers rescued the UVB-induced differentiation defect PMID: 24594668
  12. The data showed that serum autoantibodies of patients, previously identified as Dsg1 and Dsg3 positive, are able to recognize continuous linear epitope regions of both Dsg1 and Dsg3 proteins using pin-bound overlapping peptides in modified ELISAs. PMID: 23297065
  13. The loss of Bcr or MAL reduced levels of Dsg1 mRNA in keratinocytes, and ectopic expression of Dsg1 rescued defects in differentiation seen upon loss of Bcr or MAL signaling. PMID: 23940119
  14. DSG1 deficiency was associated with increased expression of a number of genes encoding allergy-related cytokines PMID: 23974871
  15. IgG autoantibodies against Dsg1 are mostly raised against preDsg1 and/or C-terminal domains of Dsg1 in healthy Tunisians in the endemic area of pemphigus foliaceus. PMID: 23489520
  16. DSG1 and Erbin cooperate to repress MAPK signaling and promote keratinocyte differentiation. PMID: 23524970
  17. we show that the LJM11 salivary protein from the sand fly are recognized by pemphigus foliaceus autoantibodies against desmoglein 1 PMID: 22798673
  18. Induced gene expression levels of plakoglobin, desmoglein-1 and desmoglein-2 correlated significantly with dilatation of intercellular spaces and basal cell hyperplasia in esophageal mucosa of patients with gastro-oesophageal reflux disease. PMID: 22276604
  19. The identification of desmogleins 1 and 3, desmosomal adhesion glycoproteins, as targets in pemphigus, a fatal autoimmune blistering disease of the skin and mucous membranes, provided the first link between desmosomes, desmogleins, and human diseases. PMID: 22189787
  20. anti-DSG1 response in fogo selvagem patients may be initiated by sensitization to an environmental allergen PMID: 21191415
  21. KLK5 may promote metastatic dissemination of OSCC by promoting loss of junctional integrity through cleavage of desmoglein 1. PMID: 21163944
  22. Data show that desmoglein 1 expression was required for the adhesive effects of EphA2. PMID: 20861311
  23. Histone deacetylase inhibition up-regulates desmosomal cadherins and prevents the loss of adhesion induced by Dsg1 truncation. PMID: 21075858
  24. localized on corneodesmosomes involved in adhesion PMID: 20116975
  25. data demonstrate the role of epistasis between individual genes in Pemphigus foliaceus susceptibility and illustrate the genetic complexity of organ-specific autoimmune diseases; Epistasis between DSG1 and HLA class II genes PMID: 12058255
  26. Staphylococcal exfoliative toxins act as serine proteases with extremely focused molecular specificity to cleave mouse and human desmoglein 1 (Dsg1) once after glutamic acid residue 381 between extracellular domains 3 and 4. PMID: 12093888
  27. During high-level expression, keratin insertion at cell-cell contact sites was inhibited in desmoglein 1 but not in desmoglein 3, and desmoplakin was stained at cell-cell contact sites in desmoglein 3 but not in desmoglein 1. PMID: 12485422
  28. Desmoglein 1 defined more differentiated cell populations, and was expressed in epidermal suprabasal cells, the inner root sheath, and the innermost layers of the outer root sheath. PMID: 12787134
  29. Signaling pathway initiated by structural changes in the adherens junction in which adherens-junction-derived plakoglobin regulates nuclear transcription by antagonizing the binding of beta-catenin to T cell factor/lymphoid enhancer factor proteins. PMID: 12880414
  30. Specificity of exfoliative toxin cleavage of desmoglein 1 resides not only in simple amino acid sequences but also in its calcium-dependent conformation. PMID: 12880431
  31. a detailed epitope mapping reveals that the conformational epitopes recognized by IgG1 autoantibodies from these PF patients are restricted to the first 161 amino acids of Dsg1, whereas the linear epitopes are spread throughout the entire ectodomain. PMID: 14675185
  32. Dsg1 promotes the formation of intercellular adhesion complexes. PMID: 15606501
  33. a component of insect vector saliva, rather than the parasite itself may trigger an antibody response to EC-5 domain of desmoglein 1 PMID: 15610512
  34. T-cell autoreactivity against Dsg1 is seen both in patients with pemphigus foliaceus and in healthy individuals PMID: 16026580
  35. No significant relationship is revealed between genetic variants of autoantigen desmoglein 1 and pemphigus foliaceus in the Brazilian population. PMID: 16242304
  36. desmoglein 1 is a novel caspase-3 target that regulates apoptosis in keratinocytes PMID: 16286477
  37. perturbation of desmoglein 1 expression has a critical impact on the integrity of tissues experiencing strong mechanical stress. PMID: 16484817
  38. An alternative transcript of DSG1, which contains a 101-bp insertion corresponding to the 3' end of DSG1-intron 6 and introducing a stop codon in the nucleotide sequence, is described. PMID: 17056584
  39. Taken together, our results represent the first demonstration that anti-Dsg1 antibodies induce similar alterations on the subcellular distribution of Dsg1 irrespective of the disease where they come from. PMID: 17058228
  40. novel splice site mutation in the DSG1 gene in palmoplantar keratoderma PMID: 17194569
  41. Data show that both pemphigus foliaceus-IgG containing Dsg 1- but not Dsg 3-specific antibodies and pemphigus vulgaris-IgG with antibodies to Dsg 1 and Dsg 3 were effective in causing epidermal splitting in human skin and keratinocyte dissociation. PMID: 17640963
  42. IgM anti-Dsg1 are common in fogo selvagem patients in their native environment and uncommon in other pemphigus phenotypes PMID: 17960181
  43. findings show that mRNA of the DSG1 gene is present in normal thymus and that the expression of DSG1 transcript increases with age PMID: 18331528
  44. The level of Dsg1, a marker of differentiating keratinocytes, was antagonistically regulated by two Ca-independent 'novel' nPKC isoforms; i.e. it increased by the differentiation-promoting nPKCdelta and decreased by the growth-promoting nPKCepsilon. PMID: 18637128
  45. Decreased expression of desmoglein 1 is associated with a worse prognosis in head and neck squamous cell carcinoma patients PMID: 18752129
  46. Direct sequencing of cDNA derived from affected skin in one patient failed to reveal a pathogenic mutation, suggesting that SPPK results from haploinsufficiency for DSG1. PMID: 19018793
  47. The desmoglein-specific cytoplasmic region is an intrinsically disordered functional domain with an inducible structure that, along with the membrane proximal region, forms a flexible scaffold for cytoplasmic assembly at the desmosome. PMID: 19136012
  48. Mutations in the desmoglein 1 gene have been indentified in five Pakistani families with striate palmoplantar keratoderma. PMID: 19157795
  49. Pemphigus foliaceus is an autoimmune blistering skin disease characterized by the production of pathogenic IgG autoantibodies directed against desmoglein 1. PMID: 19453789
  50. Data show that Dsg1 is required for maintaining epidermal tissue integrity in superficial layers, supports keratinocyte differentiation and suprabasal morphogenesis, and is required for suppression of epidermal growth factor receptor signaling. PMID: 19546243

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Involvement in disease Palmoplantar keratoderma 1, striate, focal, or diffuse (PPKS1); Erythroderma, congenital, with palmoplantar keratoderma, hypotrichosis, and hyper IgE (EPKHE)
Subcellular Location Cell membrane, Single-pass type I membrane protein, Cell junction, desmosome
Tissue Specificity Epidermis, tongue, tonsil and esophagus.
Database Links

HGNC: 3048

OMIM: 125670

KEGG: hsa:1828

STRING: 9606.ENSP00000257192

UniGene: Hs.2633

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