Recombinant Human Glycophorin-B (GYPB)

Code CSB-CF010075HU
MSDS
Size $1620
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
GYPB
Uniprot No.
Research Area
Immunology
Alternative Names
GYPB; GPB; Glycophorin-B; PAS-3; SS-active sialoglycoprotein; Sialoglycoprotein delta; CD antigen CD235b
Species
Homo sapiens (Human)
Source
in vitro E.coli expression system
Expression Region
20-91aa
Target Protein Sequence
LSTTEVAMHTSTSSSVTKSYISSQTNGETGQLVHRFTVPAPVVIILIILCVMAGIIGTILLISYSIRRLIKA
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
10.5 kDa
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 10xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
18-23 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
This protein is a minor sialoglycoprotein in erythrocyte membranes.
Gene References into Functions
  1. through bioinformatic analysis, identified extensive variation in GYPB transcript levels in individuals from Benin, suggesting selection from malaria pressure; collective data suggest that the GPA and GPB receptors are of greater importance than the GPC receptor, supporting a hierarchy of erythrocyte receptor usage in P. falciparum PMID: 28760933
  2. A novel GYPB mutation (c.270+5G>A) accounting for the S-s-U+(var) phenotype was identified. PMID: 24738877
  3. Substitution of GPB with Gp.Mur significantly reduced the expression of Rh antigen and RhAG on the Mi.III(+/+) erythrocyte membrane PMID: 21883272
  4. an increased susceptibility to infection by this parasite is associated with the glycophorin B S+ variant in Brazilian Amazons PMID: 21283638
  5. Indochina I strain of P. falciparum is not dependent on glycophorin B to invade erythrocytse through a trypsin-resistant pathway as are the strains 3D7, HB3, and Dd2. PMID: 14638759
  6. The S-s-U+var phenotype arises from changes in or around GYPB exon 5. PMID: 14641872
  7. Alternative to conventional tube technique for mass screening for MNS hybrids, especially when specific antisera are not available. PMID: 17561857
  8. Immunoblotting showed the presence of monomer and dimer forms of a GP(A-B) hybrid and an absence of GPA and GPB. Sequencing of DNA and PCR-RFLP using the restriction enzyme RsaI confirmed the presence of a hybrid GYP(AB). PMID: 18284304
  9. The erythrocyte-binding domain, region 2 of EBL-1, bound glycophorin B(+) but PMID: 19279206
  10. This study reports for the first time the molecular mechanisms responsible for the S-s- phenotype in a population of African Brazilians and provides new information about the frequency and molecular bases of the GYPB*S silent gene in this population. PMID: 19856717

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Subcellular Location
Cell membrane; Single-pass type I membrane protein.
Protein Families
Glycophorin-A family
Database Links

HGNC: 4703

OMIM: 111740

KEGG: hsa:2994

STRING: 9606.ENSP00000427690

UniGene: Hs.654368

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