Recombinant Human Microsomal glutathione S-transferase 2 (MGST2)

Code CSB-CF013792HU
MSDS
Size $1620
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  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-CF013792HU could indicate that this peptide derived from E.coli-expressed Recombinant Human Microsomal glutathione S-transferase 2(MGST2) MGST2.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-CF013792HU could indicate that this peptide derived from E.coli-expressed Recombinant Human Microsomal glutathione S-transferase 2(MGST2) MGST2.
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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
MGST2
Uniprot No.
Research Area
Immunology
Alternative Names
MGST2; GST2; Microsomal glutathione S-transferase 2; Microsomal GST-2; Glutathione peroxidase MGST2; Leukotriene C4 synthase MGST2; Microsomal glutathione S-transferase II; Microsomal GST-II
Species
Homo sapiens (Human)
Source
in vitro E.coli expression system
Expression Region
1-147aa
Target Protein Sequence
MAGNSILLAAVSILSACQQSYFALQVGKARLKYKVTPPAVTGSPEFERVFRAQQNCVEFYPIFIITLWMAGWYFNQVFATCLGLVYIYGRHLYFWGYSEAAKKRITGFRLSLGILALLTLLGALGIANSFLDEYLDLNIAKKLRRQF
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
19.4 kDa
Protein Length
Full Length
Tag Info
N-terminal 10xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

Customer Reviews and Q&A

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 Q&A
Q:

Kindly mention the source and the expression host of the product

A:
Thanks for your inquiry! The Source: in vitro E.coli expression
Expression Host: E.coli extract

Target Background

Function
Catalyzes several different glutathione-dependent reactions. Catalyzes the glutathione-dependent reduction of lipid hydroperoxides, such as 5-HPETE. Has glutathione transferase activity, toward xenobiotic electrophiles, such as 1-chloro-2, 4-dinitrobenzene (CDNB). Catalyzes also the conjugation of leukotriene A4 with reduced glutathione to form leukotriene C4 (LTC4). Involved in oxidative DNA damage induced by ER stress and anticancer agents by activating LTC4 biosynthetic machinery in nonimmune cells.
Gene References into Functions
  1. Trimeric microsomal protein MGST2 displays one third of the sites reactivity. PMID: 26066610
  2. characterized the substrate specificity and catalytic properties of purified MGST2 by steady-state and pre-steady-state kinetic experiments PMID: 23409838
  3. A large Chinese sample of psoriasis did not provide any supporting evidence for MGST2 to be the psoriasis susceptibility gene within the PSORS9 locus. PMID: 16773312
Subcellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein. Microsome membrane; Multi-pass membrane protein.
Protein Families
MAPEG family
Tissue Specificity
Liver, spleen, skeletal muscle, heart, adrenals, pancreas, prostate, testis, fetal liver, and fetal spleen. Very low expression in lung, brain, placenta and bone marrow. Abundantly expressed in human umbilical vein endothelial cells (at protein level).
Database Links

HGNC: 7063

OMIM: 601733

KEGG: hsa:4258

STRING: 9606.ENSP00000265498

UniGene: Hs.81874

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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