Recombinant Human Ribonuclease kappa (RNASEK)

Code CSB-CF761293HU
MSDS
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names
RNASEK
Uniprot No.
Alternative Names
RNASEK; Ribonuclease kappa; RNase K; RNase kappa
Species
Homo sapiens (Human)
Expression Region
1-98
Target Protein Sequence
MASLLCCGPKLAACGIVLSAWGVIMLIMLGIFFNVHSAVLIEDVPFTEKDFENGPQNIYN LYEQVSYNCFIAAGLYLLLGGFSFCQVRLNKRKEYMVR
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
full length protein
Tag Info
N-terminal 10xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Endoribonuclease which preferentially cleaves ApU and ApG phosphodiester bonds. Hydrolyzes UpU bonds at a lower rate.; (Microbial infection) Required for the initial stages of clathrin-mediated endocytic uptake of a diverse set of viruses, including dengue, West Nile, Sindbis, Rift Valley Fever, and influenza viruses. Not required for clathrin-mediated endocytosis and macropinocytosis.
Gene References into Functions
  1. Low Rnase K expression is associated with prostate cancer. PMID: 30054827
  2. Analyses of global gene expression data from adipose tissue, skeletal muscle, and whole blood provide evidence that rs75493593 is associated with RNASEK gene expression, suggesting that RNASEK expression may mediate the effect of genotype on diabetes. PMID: 26487785
  3. RNASEK closely associates with the V-ATPase and is required for its function; its loss prevents the early events of endocytosis and the replication of multiple pathogenic viruses. PMID: 26212330
  4. Data suggest that the Cys6-Cys69 disulfide bond is likely to exist in all RNase kappa family members, including human. PMID: 22324914
  5. Observational study of gene-disease association. (HuGE Navigator) PMID: 19913121
  6. Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator) PMID: 20628086
  7. Based on sequence alignment and substrate specificity data, as well as the resistance of the protein to the placental ribonuclease inhibitor, the human RNase kappa may be a novel endoribonuclease distinct from other known ribonucleases. PMID: 17881363

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Subcellular Location
Membrane; Multi-pass membrane protein.
Protein Families
RNase K family
Tissue Specificity
Widely expressed.
Database Links

HGNC: 33911

KEGG: hsa:440400

STRING: 9606.ENSP00000449500

UniGene: Hs.632232

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