Recombinant Human T-lymphocyte activation antigen CD80(CD80)

Code CSB-CF1054HU
Size US$3313
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 85% as determined by SDS-PAGE.
Target Names CD80
Uniprot No. P33681
Research Area Immunology
Alternative Names Activation B7-1 antigen; B lymphocyte activation antigen B7; B7; B7-1; B7-1 antigen; B7.1; BB1; CD28 antigen ligand 1; CD28LG; CD28LG1; CD80; CD80 antigen (CD28 antigen ligand 1; B7-1 antigen); CD80 antigen; CD80 molecule; CD80_HUMAN; Costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; LAB7; T-lymphocyte activation antigen CD80
Species Homo sapiens (Human)
Source in vitro E.coli expression system
Expression Region 35-288aa
Target Protein Sequence VIHVTKEVKEVATLSCGHNVSVEELAQTRIYWQKEKKMVLTMMSGDMNIWPEYKNRTIFDITNNLSIVILALRPSDEGTYECVVLKYEKDAFKREHLAEVTLSVKADFPTPSISDFEIPTSNIRRIICSTSGGFPEPHLSWLENGEELNAINTTVSQDPETELYAVSSKLDFNMTTNHSFMCLIKYGHLRVNQTFNWNTTKQEHFPDNLLPSWAITLISVNGIFVICCLTYCFAPRCRERRRNERLRRESVRPV
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 32.8 kDa
Protein Length Full Length of Mature Protein
Tag Info N-terminal 10xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation.
Gene References into Functions
  1. Addition of TNFalpha to podocytes causes CD80 upregulation, actin reorganization and podocyte injury. PMID: 29022109
  2. Urinary CD80 levels were significantly higher in the children with minimal change disease than in the controls and patients with other causes of nephrotic syndrome. PMID: 29507273
  3. Results showed novel genetic associations with bone phenotypes and points to the CD80 gene as relevant in postmenopausal bone loss. PMID: 28466138
  4. Increased expressions of TLR-3, TLR-4 and CD80 mRNA and the level of urinary CD80/creatinine could be useful markers to differentiate patients of steroid-sensitive nephrotic syndrome in relapse from those with steroid-resistant nephrotic syndrome. PMID: 28210837
  5. The rs1915087 (C>T), rs6804441 (A>G) and rs41271391 (G>T) of B7 antigens were suspected as the factors associated with reduced risk of recurrent spontaneous abortion. PMID: 29069644
  6. Tregs were observed to regulate CD4(+), but not CD8(+), T cell infiltration into tumors through a CTLA-4/CD80 dependent mechanism. Disrupting CTLA-4 interaction with CD80 was sufficient to induce CD4 T cell infiltration into tumors. PMID: 28856392
  7. Results show that CD80 down-regulation is associated to aberrant DNA methylation in dysplasia of sporadic colonic carcinogenesis. This study indicates that the failure of immune surveillance mechanisms in non-inflammatory colon carcinogenesis may be linked to genomic methylation directly or indirectly affecting CD80 expression. PMID: 27377375
  8. Fabry disease is characterized by early occurrence of increased uCD80 excretion that appears to be a consequence of glycolipid accumulation. PMID: 27733175
  9. CTLA-4(+) microvesicles can competitively bind B7 costimulatory molecules on bystander dendritic cells, resulting in downregulation of B7 surface expression. PMID: 26979751
  10. The expression of B7-H6 is up-regulated in U87-derived glioma stem like cells. PMID: 27609569
  11. PD-1 receptor has a role in interacting with programmed cell death ligands and B7-1 PMID: 28270509
  12. CD80-QPAR platform provides a useful predictive model for unknown RA extract's bioactivities using the chemical fingerprint inputs PMID: 28337449
  13. this study shows that dendritic cells from rheumatoid arthritis patients have low expression levels of CD80 PMID: 27421624
  14. B7-1 is not expressed by podocytes in LN. A renoprotective effect of B7-1 blockade in LN patients cannot be ruled out but, if confirmed, cannot be the result of an effect on podocyte B7-1 PMID: 27198457
  15. analysis of CTLA4-Ig in B7-1-positive diabetic and non-diabetic kidney disease [review] PMID: 26409459
  16. Genetic interaction of CD80 and ALOX5AP was observed in systemic lupus erythematosus in Asian populations. PMID: 25862617
  17. Inhibitory Profile of Liver CD68+ Cells during HCV Infection as Observed by an Increased CD80 and PD-L1 but Not CD86 Expression PMID: 27065104
  18. Triple costimulation via CD80, 4-1BB, and CD83 ligand elicits the long-term growth of Vgamma9Vdelta2 T cells in low levels of IL-2. PMID: 26561569
  19. B7-1 is not induced in podocytes from patients with minimal change disease or focal segmental glomerulosclerosis. PMID: 26697986
  20. In the present study, we investigated the prognostic significance of the expression of three genes in the PD-L1 pathway, including PD-L1, B7.1 and PD-1, in three independent bladder cancer datasets in the Gene Expression Omnibus database. PMID: 25963805
  21. SNP rs1599795 in CD80 3'-UTR, through disrupting the regulatory role of miR-132-3p, miR-212-3p, and miR-361-5p in CD80 expression, contributed to the occurrence of gastric cancer. PMID: 24981235
  22. Meningococcal capsular polysaccharide-loaded vaccine nanoparticles induce expression of CD80. PMID: 24981893
  23. These findings reveal the distinct but complementary roles of CD80 and CD86 IgV and IgC domains in T cell activation. PMID: 24845157
  24. Expression of costimulatory molecules CD80/86 is an absolute requirement for efficient CD8 T cell priming by adenoviral vectors. PMID: 24951814
  25. Single-nucleotide polymorphisms in CD80 gene is associated with breast cancer risk after menopausal hormone replacement therapy. PMID: 24080446
  26. Data indicate that STAT5A and STAT5B transcription activator complex induces expression of CD80 gene. PMID: 24523507
  27. NOTCH1 protein regulates CD80/CD86-induced phosphatidylinositol 3-kinase signaling in interleukin-6 and indoleamine 2,3-dioxygenase production by dendritic cells PMID: 24415757
  28. lower expression on CD1c+ myeloid and CD303+ plasmacytoid DCs in pre-eclampsia PMID: 23773232
  29. No role in CD80 expression by podocytes was found for cytokines released by peripheral blood mononuclear cells PMID: 23689904
  30. Data indicate that low-dose decitabine (DAC) treatment can induce CD80 gene expression in a variety of cancer cells. PMID: 23671644
  31. These studies identify CD80-Fc as an alternative and potentially more efficacious therapeutic agent for overcoming PDL1-induced immune suppression and facilitating tumor-specific immunity PMID: 23918985
  32. Data indicate that the frequencies of CD11c, CD11c/CD86, HLA-DR/CD86, CD83 and CD80 were significantly high, while CD11c/HLA-DR was low in Hepatitis E infection. PMID: 23246582
  33. Lower expression of B7-1 and B7-2 proteins on peripheral monocytes in pre-eclampsia might indicate a secondary regulatory mechanism in response to the ongoing systemic maternal inflammation. PMID: 23289444
  34. he aim of this work was to analyse the interaction between early events in colonic ulcerative colitis-related and non-inflammatory carcinogenesis and CD80 expression to clarify what stimuli induce its up-regulation in these patients. PMID: 22704122
  35. mRNA expression analysis of B7-1 and NPHS1 in urinary sediment may be useful to differentiate between different histologic subtypes of glomerular kidney disease, particularly between minimal change disease and focal segmental glomerulosclerosis. PMID: 21414970
  36. Data suggest that expression of CD80, CD86, and CD40 on dendritic cells in normal endometrium is higher than on tumor infiltrating dendritic cells in endometrioid adenocarcinoma; this may reflect roles in antigen presentation/tumor escape. PMID: 22142817
  37. Data show that CD80 promoter and CD86 exon 8 allele frequencies vary significantly among populations of different ancestries. PMID: 22074996
  38. The interaction between PDL1 on antigen prresenting cells and B7.1 on T cells plays a dominant role in bidirectional interactions between these two molecules during alloimmune responses. PMID: 21697455
  39. the expression of the co-stimulatory molecule CD80 was decreased in intestines of celiac disease children after gluten-free diet. PMID: 21288140
  40. the costimulatory molecule CD80 prevents PDL1-mediated immune suppression by tumor cells and restores T cell activation PMID: 21555531
  41. Efficiency of GHA priming chemotherapy on refractory acute myeloid leukemia and myelodysplastic syndrome may be correlated with B7.1 expression. PMID: 18928583
  42. The low expression of CD80 and CD86 in thyroid papillary carcinoma may help them evade the immune system. PMID: 21469977
  43. study shows CTLA-4 can capture its ligands (CD80, CD86)from opposing cells by trans-endocytosis; data reveal mechanism of immune regulation in which CTLA-4 acts as an effector molecule to inhibit CD28 costimulation by cell-extrinsic depletion of ligands PMID: 21474713
  44. These results suggest that the polymorphisms of the CD86 gene may be used as genetic markers for making the diagnosis and prognosis of Graves' ophthalmopathy. PMID: 20884055
  45. Thalidomide can up-regulate the expression of B7-1 molecules on myeloma cells. PMID: 20034904
  46. increased CD80 and CD86 expression with the progression of tubulointerstitial lesion might play an important role in the development of lupus nephropathy PMID: 20979791
  47. B7-1 costimulation is required for induction and maintenance of lymphocytic choriomeningitis virus (LCMV)-specific CD8+ T cell memory, in T cell receptor (TCR)transgenic mice. PMID: 20601595
  48. B7-H1 and B7-1 significantly correlated with the pathological grade and tumor-node-metastasis (TNM) stage, respectively in pancreatic cancer. PMID: 20145927
  49. Data show that pollen grains triggered the production of IL-8, TNF-alpha, IL-6 and strongly upregulated the membrane expression of CD80, CD86, CD83, HLA-DR and caused only a slight increase in the expression of CD40. PMID: 20118277
  50. Thus, this study is the first demonstration of a distinct signaling event induced by CD80 and CD86 molecules in B cell lymphoma. PMID: 11726649
  51. specific small molecule inhibitors of human B7.1 were identified and characterized. These compounds inhibit the binding of B7.1 to both CD28 and CTLA4. PMID: 11741888
  52. colocalization of ICAM-1 and B7.1 molecules was demonstrated in Hashomoto's thyroiditis whereas no B7.1 expression was observed in Graves' disease PMID: 11936473
  53. CD80 and CD86 molecules can substitute for each other in the initial activation of resting CD4(+) T cells and in the maintenance of their proliferative response PMID: 12015893
  54. Leishmania major infection of macrophages cocultured with neutrophils results in a neutrophil-macrophage interaction via CD80 leading to IFN-gamma secretion and restriction of Leishmania growth. PMID: 12097397
  55. expansion of CD5- B cells in multiple sclerosis correlates with CD80 (B7-1) expression PMID: 12100477
  56. CD40 and CD80 molecules were observed to play a specific role in the induction of cytotoxic function but not in IFN-gamma production of IL-2-activated NK effectors. PMID: 12149421
  57. with ctla4 pathway, is essential for generating regulatory cells after intratracheal delivery of alloantigen in mice PMID: 12352894
  58. CD80 and CD86 differ in their interactions with CTLA-4 and that CD80 appears to be the preferential inhibitory ligand for CTLA-4 working via a population of CD4(+) CD25(+) CTLA-4(+) regulatory T cells. PMID: 12355442
  59. After B7-1 and B7-2 induction, proximal tubular epithelial cells costimulate CD28 on T lymphocytes resulting in cytokine production. PMID: 12372936
  60. data suggest that CD80 acquisition by human T cells might play a role in the immunoregulation of T cell responses PMID: 12444120
  61. The identical effects of B7-1 and B7-2 on regulation of human IL-2 gene transcription factors NF-kappa B and AP-1. PMID: 12513711
  62. On the biological relevance of MHC class II and B7 expression by tumour cells in melanoma metastases. PMID: 12569387
  63. combined use of a vector driving the expression of OX40L with three other costimulatory molecules (B7-1, ICAM-1, and LFA-3) both enhances initial activation and then further potentiates sustained activation of nai;ve and effector T cells. PMID: 12798307
  64. ICOS-B7H costimulatory pathway may be involved in the negative regulation of cell-mediated immune responses. PMID: 12800259
  65. expression profiles and relative contribution in the porcine-human xenogeneic response PMID: 12829914
  66. effect of PGE2 on the expression of ICAM-1 and B7 in the human mixed leukocyte reaction (MLR) in the presence or absence of IL-18 PMID: 12829919
  67. results demonstrate that herpes simplex virus-2 infection effects the expression of B7 isoforms (B7-1 and B7-2) on monocytes in two ways with opposing outcomes PMID: 12878356
  68. Immature dendritic cells engulfed apoptotic and necrotic neutrophils, resulting in up-regulation of CD83 and class II major histocompatibility complex molecules, but down-regulation of CD40, CD80, and CD86 PMID: 12905492
  69. The CD80 immunoglobulin constant-like (C)-domain has a negative/regulatory effect on the immune response to a plasmid DNA vaccine; loss of the CD80 C-domain prevents modulation of the immune activation signal. PMID: 14530356
  70. results confirm that both the CD80 and CD86 molecules play an important role in the maintenance and amplification of the inflammatory process PMID: 14692664
  71. CD80 and CD86 activate T cells in IgA nephropathy, CD80/CD86 expressions correlated with renal function at the time of renal biopsy, and monocyte/macrophages and tubular epithelial cells act as antigen presenting cells PMID: 14871408
  72. CD80 and CD86 have opposite roles in the functioning of human regulatory T cells via CD28 and CTLA-4, an observation that has significant implications for manipulation of immune responses and tolerance in vivo. PMID: 14978077
  73. Engagement of B7-1/B7-2 molecules on human indoleamine 2,3-dioxygenase (IDO)-positive dendritic cells delivers a signal that is obligately required for the triggering of IDO activity during antigen presentation. PMID: 15034022
  74. utilized as cellular attachment receptor by Adenovirus serotype 3 and thus may achieve both goals of cellular entry and evasion of the immune system PMID: 15110532
  75. insertion of CD80 and CD86 antigens into HIV-1 virions increases virus infectivity by facilitating the attachment and entry process due to interactions with their two natural ligands, CD28 and CTLA-4 PMID: 15163715
  76. B7-1 gene transduction might be effective against peritoneal metastases of gastric cancer. PMID: 15201958
  77. CD80 has a role in activating cytotoxic T lymphocytes in hepatocellular carcinoma cells PMID: 15254713
  78. CD80 driven by NF-kappaB is regulated by the enzymatic actions of caspases, which allows monocytes to participate in massive T-cell activation PMID: 15254772
  79. data demonstrated a correlation between HIV infection and impairment of CD80 by circulating monocytes PMID: 15272203
  80. There is a potential role of B7 co-stimulatory molecules in facilitating hepatocellular carcinoma escape from immune surveillance without the involvement of IL-10. PMID: 15377288
  81. CD80 binding polyproline helical peptide has a role inhibiting T cell activation PMID: 15598660
  82. Upregulation in the colonic mucosa of patients with ulcerative colitis. PMID: 15628695
  83. There was no relationship between the B7 gene polymorphisms studied and disease susceptibility or BAL fluid cell profiles in Japanese sarcoidosis patients. PMID: 15942292
  84. B7.1/B7.2 binding ultimately determines the formation of dimer-dependent CTLA-4 lattices that may be necessary for triggering B7-dependent T cell inactivation. PMID: 16002699
  85. LPS-induced B7.1 transcription in human monocytic cells may be regulated by JNK-mediated activation of the IRF-7 transcription factor. PMID: 16237059
  86. Th2 cytokine predominant in tumor microenvironment might be related to the expression of B(7)H(1),B(7)H(2) co-signal molecules in tumor cells and TIL PMID: 16237760
  87. CD80 transfection can lower malignant phenotype of hepatocarcinoma cells and has a down-regulatory effect to activated T cells in vitro. PMID: 16521215
  88. CD80-CD24 molecule spontaneously incorporated onto cell membrane through its glycolipid anchor suggesting that this hybrid costimulatory molecule can be used in protein transfer to develop effective cancer vaccines PMID: 16621031
  89. While purified CD123+ plasmacytoid dendritic cells from adults up-regulated co-stimulatory molecules CD80 and CD86 with IL-3 alone those from neonates required the addition of CpG-DNA to reach adult levels. PMID: 16690948
  90. CD80 expression during early oral squamous cell carcinoma formation may contribute to the escape of tumors from immune elimination PMID: 17201120
  91. Transduction of HMCLs with B7-1 retroviruses induced a high expression of B7-1 molecule and a strong T-cell activation ability. PMID: 17309825
  92. These results define elevated populations of memory T-lymphocytes expressing B7 molecules in rheumatoid arthritis (RA) synovial fluid that either stimulate T cells through ICOS, or down-regulate RA synovial tissue T-lymphocytes through B7H1 and B7H2. PMID: 17323353
  93. Expression of CD80 and CD86 costimulatory molecules appears to be a marker of better clinical outcome and survival in patients with nasopharyngeal carcinoma. PMID: 17524139
  94. The expression of CD80 and CD86 on peripheral lymphocytes from idiopathic thrombocytopenic purpura patients were higher than that of the normal control. PMID: 17605869
  95. CD40, CD80 and CD86 are upregulated in cultured monocyte-derived dendritic cells of patients with coronary artery disease PMID: 17925605
  96. sCD80 in human serum adds a new member to the family of soluble receptors, implying a network of soluble costimulatory factors with functional relevance PMID: 17953528
  97. T cells that had become non-responsive to anti-CD3 could be reactivated to proliferate when costimulated with 4-1BBL, either alone or combined with CD80/CD86. PMID: 17977894
  98. CD80 expression was up-regulated on the circulating monocytes in septic subjects on Day 1 PMID: 17989345
  99. Results describe a two-pronged mechanism by which Nef removes CD80 and CD86 from the cell surface. PMID: 18005680
  100. CD80 and 4-1BBL induce auto- and transcostimulation in tumor cells PMID: 18026115

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Subcellular Location Membrane, Single-pass type I membrane protein
Tissue Specificity Expressed on activated B-cells, macrophages and dendritic cells.
Database Links

HGNC: 1700

OMIM: 112203

KEGG: hsa:941

STRING: 9606.ENSP00000264246

UniGene: Hs.838

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