CD80

CD80, also known as B7-1 or BB1, is a crucial costimulatory molecule expressed on various cell types, including activated B cells, T cells, macrophages, and tumor cells. Its primary function is to interact with CD28 on T cells, facilitating T-cell activation and proliferation. CD80 is involved in several signaling pathways, including those involving PD-L1 and CTLA-4, which regulate immune responses and tumor growth. CD80 expression is associated with various diseases, such as multiple sclerosis, systemic lupus erythematosus, and certain types of cancer. In drug development, CD80-targeted therapies, including fusion proteins, monoclonal antibodies, and CAR T-cell therapies, are being investigated for their potential in treating cancer and autoimmune diseases.

Validated Data

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

CD80 Recombinant Monoclonal Antibody (CSB-RA246383A0HU)

Validated Data

Western Blot
Positive WB detected in: Raji whole cell lysate
All lanes: CD80 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 34, 30, 19 kDa
Observed band size: 60 kDa

IHC image of CSB-RA246383A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Immunoprecipitating CD80 in Raji whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-RA246383A0HU in Raji whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-RA246383A0HU(2µg)+ Raji whole cell lysate(500µg)
Lane 3: Raji whole cell lysate (10µg)

The following CD80 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.

CD80 Antibodies

CD80 Proteins

CD80 ELISA Kits

CD80 Antibodies

CD80 Antibodies for Homo sapiens (Human)

Code	Product Name	Species Reactivity	Application
CSB-PA004959GA01HU	CD80 Antibody	Human	ELISA,WB
CSB-PA277650	CD80 Antibody	Human	ELISA,IHC
CSB-PA130815	CD80 Antibody	Human	ELISA,IHC
CSB-PA129040	CD80 Antibody	Human	IHC,ELISA
CSB-RA246383A0HU	CD80 Recombinant Monoclonal Antibody	Human	ELISA, WB, IHC, IP

CD80 Proteins

CD80 Proteins for Homo sapiens (Human)

Code	Product Name	Source
CSB-CF004959HU	Recombinant Human T-lymphocyte activation antigen CD80 (CD80)	in vitro E.coli expression system
CSB-MP004959HU1	Recombinant Human T-lymphocyte activation antigen CD80 (CD80), partial	Mammalian cell
CSB-YP004959HU1 CSB-EP004959HU1 CSB-BP004959HU1 CSB-MP004959HU1 CSB-EP004959HU1-B	Recombinant Human T-lymphocyte activation antigen CD80 (CD80), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-CF1054HU	Recombinant Human T-lymphocyte activation antigen CD80 (CD80)	in vitro E.coli expression system
CSB-MP1054HU	Recombinant Human T-lymphocyte activation antigen CD80 (CD80)-VLPs	Mammalian cell

CD80 Proteins for Oryctolagus cuniculus (Rabbit)

Code	Product Name	Source
CSB-CF004959RB	Recombinant Rabbit T-lymphocyte activation antigen CD80 (CD80)	in vitro E.coli expression system
CSB-YP004959RB1 CSB-EP004959RB1 CSB-BP004959RB1 CSB-MP004959RB1 CSB-EP004959RB1-B	Recombinant Rabbit T-lymphocyte activation antigen CD80 (CD80), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CD80 Proteins for Mus musculus (Mouse)

Code	Product Name	Source
CSB-CF004959MO	Recombinant Mouse T-lymphocyte activation antigen CD80 (Cd80)	in vitro E.coli expression system
CSB-YP004959MO1 CSB-EP004959MO1 CSB-BP004959MO1 CSB-MP004959MO1 CSB-EP004959MO1-B	Recombinant Mouse T-lymphocyte activation antigen CD80 (Cd80), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli