CD80, also known as B7-1 or BB1, is a crucial costimulatory molecule expressed on various cell types, including activated B cells, T cells, macrophages, and tumor cells. Its primary function is to interact with CD28 on T cells, facilitating T-cell activation and proliferation. CD80 is involved in several signaling pathways, including those involving PD-L1 and CTLA-4, which regulate immune responses and tumor growth. CD80 expression is associated with various diseases, such as multiple sclerosis, systemic lupus erythematosus, and certain types of cancer. In drug development, CD80-targeted therapies, including fusion proteins, monoclonal antibodies, and CAR T-cell therapies, are being investigated for their potential in treating cancer and autoimmune diseases.
Recombinant Human T-lymphocyte activation antigen CD80 (CD80), partial (CSB-MP004959HU1)
Validated Data
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
CD80 Recombinant Monoclonal Antibody (CSB-RA246383A0HU)
Validated Data
Western Blot
Positive WB detected in: Raji whole cell lysate
All lanes: CD80 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 34, 30, 19 kDa
Observed band size: 60 kDa
IHC image of CSB-RA246383A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunoprecipitating CD80 in Raji whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-RA246383A0HU in Raji whole cell lysate.
For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-RA246383A0HU(2µg)+ Raji whole cell lysate(500µg)
Lane 3: Raji whole cell lysate (10µg)
The following CD80 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
CD80 Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA004959GA01HU | CD80 Antibody | Human | ELISA,WB |
CSB-PA277650 | CD80 Antibody | Human | ELISA,IHC |
CSB-PA130815 | CD80 Antibody | Human | ELISA,IHC |
CSB-PA129040 | CD80 Antibody | Human | IHC,ELISA |
CSB-RA246383A0HU | CD80 Recombinant Monoclonal Antibody | Human | ELISA, WB, IHC, IP |
CD80 Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-CF004959HU | Recombinant Human T-lymphocyte activation antigen CD80 (CD80) | in vitro E.coli expression system |
CSB-MP004959HU1 | Recombinant Human T-lymphocyte activation antigen CD80 (CD80), partial | Mammalian cell |
CSB-YP004959HU1 CSB-EP004959HU1 CSB-BP004959HU1 CSB-MP004959HU1 CSB-EP004959HU1-B |
Recombinant Human T-lymphocyte activation antigen CD80 (CD80), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-CF1054HU | Recombinant Human T-lymphocyte activation antigen CD80 (CD80) | in vitro E.coli expression system |
CSB-MP1054HU | Recombinant Human T-lymphocyte activation antigen CD80 (CD80)-VLPs | Mammalian cell |
CD80 Proteins for Oryctolagus cuniculus (Rabbit)
Code | Product Name | Source |
---|---|---|
CSB-CF004959RB | Recombinant Rabbit T-lymphocyte activation antigen CD80 (CD80) | in vitro E.coli expression system |
CSB-YP004959RB1 CSB-EP004959RB1 CSB-BP004959RB1 CSB-MP004959RB1 CSB-EP004959RB1-B |
Recombinant Rabbit T-lymphocyte activation antigen CD80 (CD80), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CD80 Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-CF004959MO | Recombinant Mouse T-lymphocyte activation antigen CD80 (Cd80) | in vitro E.coli expression system |
CSB-YP004959MO1 CSB-EP004959MO1 CSB-BP004959MO1 CSB-MP004959MO1 CSB-EP004959MO1-B |
Recombinant Mouse T-lymphocyte activation antigen CD80 (Cd80), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CD80 ELISA Kit for Homo sapiens (Human)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-E15768h | Human Soluble Cluster of Differentiation 80 (sCD80) ELISA Kit | serum, plasma, cell culture supernates | 31.25 pg/mL |
CD80 ELISA Kit for Mus musculus (Mouse)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-EL004959MO | Mouse T-lymphocyte activation antigen CD80(CD80) ELISA kit | serum, plasma, tissue homogenates | 3.9 pg/mL |