Recombinant Human Tryptophan 5-hydroxylase 2(TPH2)

Code CSB-CF809000HU
Size US$1235
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names TPH2
Uniprot No. Q8IWU9
Research Area Neuroscience
Alternative Names MGC138871; ADHD7; FLJ37295; MGC138872; Neuronal tryptophan hydroxylase; NTPH; TPH 2; Tph2; TPH2_HUMAN; Tryptophan 5-hydroxylase 2; Tryptophan 5-monooxygenase 2; Tryptophan hydroxylase 2
Species Homo sapiens (Human)
Source in vitro E.coli expression system
Expression Region 1-490aa
Target Protein Sequence MQPAMMMFSSKYWARRGFSLDSAVPEEHQLLGSSTLNKPNSGKNDDKGNKGSSKREAATESGKTAVVFSLKNEVGGLVKALRLFQEKRVNMVHIESRKSRRRSSEVEIFVDCECGKTEFNELIQLLKFQTTIVTLNPPENIWTEEEELEDVPWFPRKISELDKCSHRVLMYGSELDADHPGFKDNVYRQRRKYFVDVAMGYKYGQPIPRVEYTEEETKTWGVVFRELSKLYPTHACREYLKNFPLLTKYCGYREDNVPQLEDVSMFLKERSGFTVRPVAGYLSPRDFLAGLAYRVFHCTQYIRHGSDPLYTPEPDTCHELLGHVPLLADPKFAQFSQEIGLASLGASDEDVQKLATCYFFTIEFGLCKQEGQLRAYGAGLLSSIGELKHALSDKACVKAFDPKTTCLQECLITTFQEAYFVSESFEEAKEKMRDFAKSITRPFSVYFNPYTQSIEILKDTRSIENVVQDLRSDLNTVCDALNKMNQYLGI
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 60.1kDa
Protein Length Full Length
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Gene References into Functions
  1. These findings indicate that TPH1, TPH2, and SLC6A4 variants moderate the subjective effects of cocaine in non-treatment-seeking cocaine-dependent participants. PMID: 28590957
  2. results provide new evidence for the role of regulatory genetic variants that could modulate human TPH2 expression in the antidepressant response to fluoxetine. PMID: 28456685
  3. This study firstly demonstrated evidence for an association between aberrant cortical folding in the PFC and ACC and TPH2 polymorphism gene related to the pathophysiology of major depressive disorder. PMID: 27807918
  4. This study shown that theTPH2 genotype is likely to modulate the risk for addictive behaviors in PD on dopaminergic therapy. PMID: 27237108
  5. social anxiety disorder entails an overactive presynaptic serotonergic system that, in turn, seems functionally influenced by the TPH2 G-703T polymorphism in emotionally relevant brain regions PMID: 27189957
  6. nine ADHD candidate single nucleotide polymorphisms (SNPs) in seven genes were tested for association with PD in 5333 cases and 12,019 healthy controls. No significant association was observed. PMID: 28176268
  7. Tryptophan hydroxylase synthesizes Serotonin and is over-expressed in Suicides.TPH2 mRNA was greater in Suicide Nonsmokers than Suicide Smokers.The absence of altered TPH2 expression in non-suicide smokers suggests no pharmacological effect of Smoking. PMID: 26954509
  8. This study shown the significant results were found with TPH2 rs4570625 involved with fast visual processing as assessed by metacontrast. PMID: 27926628
  9. Genetic variation in TPH2 is associated with antidepressant response and may also interact with childhood trauma to influence outcome of antidepressant treatment. PMID: 27521242
  10. 2-way interaction between TPH2 rs4290270 and general traumas revealed that TT homozygotes with history of general traumas had an increased risk for suicide attempt. 3-way interaction of general traumas, TPH2 rs4290270 and ADARB1 rs4819035 indicated that highest predisposition to suicide attempt was observed in individuals who experienced general traumas and were TT homozygote for rs4290270 and TT homozygote for rs4819035. PMID: 28084537
  11. The catalytic domain of TPH1 shares a sequence identity of 81% with TPH2. Despite the high sequence identity, differences in the kinetic parameters of the isoforms have been identified; i.e., only TPH1 displays substrate tryptophan inhibition. PMID: 29035515
  12. results confirm the role of TPH1, TPH2, 5HT2A, CRHR1 and ACP1 variants in the risk of suicidal behavior. PMID: 27479537
  13. There was no association between rs11179000, rs136494 and rs4570625 polymorphisms of the tryptophan hydroxylase 2 gene and major depressive disorder in a sample of the Colombian population PMID: 28193370
  14. This study demonstrates that both TPH1 and TPH2 are expressed in human and mouse placenta throughout pregnancy and helps to better understand the placental serotonin system, which is crucial for healthy pregnancy and fetal development. PMID: 28751217
  15. This study provides the first evidence that, at least among Han Chinese patients with PD, AC genotype at rs78162420 in the TPH2 gene increase risk of depression. PMID: 28320136
  16. interaction between SNP rs4570625 and BDNF Val66Met predicts a high-Risk emotional phenotype PMID: 27695066
  17. Subjects with the TPH2 rs4570625 TT genotype, especially males, exhibit less aggression and a favourable impulsivity profile, and develop anxiety disorders by young adulthood less often PMID: 28342337
  18. Study reports an association between functional variation on the TPH2 promotor region and rich club-a densely mutually connected set of hub regions in the brain, thought to serve as a processing and integration core-organization PMID: 26975194
  19. Altered TPH2 expression in colonic serotonergic nerves of patients with Hirschsprung's disease complicated by enterocolitis may contribute to intestinal secretory and motor disturbances, including recurrent HAEC. PMID: 27217698
  20. The purposes of this study were to investigate the potential association of TPH-2 polymorphisms with susceptibility to ODD in a Han Chinese school population. In single marker analyses,there was a significant difference in the genotype (chi (2) = 4.163, P = 0.041) and allele frequency (chi (2) = 3.930, P = 0.047) of rs1386494 between ODD and control groups. PMID: 27871272
  21. the association studies between TPH2 and Bipolar disorder was investigated. PMID: 26365518
  22. Carriers of the TPH-2 allele may be at increased risk for PTSD. COMT rs4633C and TPH-2 rs11178997T alleles jointly explained 7% of the variance in PTSD severity scores. These findings provide biological support for dividing DSM-IV category C symptoms into DSM-5 categories C and D. PMID: 25451452
  23. Study demonstrated that 5-HTTLPR, VNTR In2, and TPH2 rs1386497 polymorphisms mediate working memory performance and that their effects on working memory depend on cocaine use of participants PMID: 26013962
  24. We also found that early family structure directly influences the levels of dysregulation problems in early adolescence, both alone and in interaction with TPH2. PMID: 26583347
  25. Chinese Han individuals with at least oners11178997T allele or rs120074175A allele are susceptible to MD even in the relative absence of high-negative life events. PMID: 26386440
  26. loss of function mutation in Tph2 results in greater motivation for ethanol consumption under aversive conditions and may confer enhanced sensitivity to alcohol use disorder PMID: 26497913
  27. TPH2 expression is regulated by DNA methylation of the TPH2 promoter region in patients with major depressive disorder. PMID: 25955598
  28. results provide evidence for an association between the rs7305115 polymorphism of the TPH-2 gene and suicidal behavior in a Mexican population. PMID: 26028568
  29. mood disorders and HTR1A G allele variation, the C-1019G polymorphism of the transcriptional control region of the 5-HT1A receptor, independently predicted the incidence of IFN-induced depression in HCV patients PMID: 26001668
  30. A potential role of TPH2 in alcohol dependencies suggested. TPH2 genetic variability may be also associated with anxiety and aggression traits in alcohol dependent subjects. PMID: 26232682
  31. 1,25-Dihydroxyvitamin D regulates expression of the tryptophan hydroxylase 2 and leptin genes: implication for behavioral influences of vitamin D. PMID: 26071405
  32. Case-control genetic association study in multi-ethnic Malaysian population. Strong linkage disequilibrium was detected between rs1386495 and rs1386494 in pooled subjects; however, no significant association was found in the haplotype analysis. PMID: 24376086
  33. TPH2 polymorphism may be associated with cognitive function in late onset depression patients. PMID: 26057341
  34. With regard to G-703 T/A-473 T/G19918A TPH-2 variants, this up-to-date meta-analysis could not detect any significant association between those genetic variants and suicidal behavior .[review and meta-analysis] PMID: 25005534
  35. genetic contribution to phenotypic facets related to obsessive-compulsive symptoms and perfectionism in healthy subjects; association of TPH2 s4570625 and DRD2 TaqIA SNPs with the subscales scrupulousness and perseverance of the BFQ factor conscientiousness were found PMID: 25283578
  36. results provide evidence on the possible role of genetic variations in 5-HT neurotransmission on development of personality traits, and suggest a functional interaction between two key proteins in the serotonergic system. PMID: 25455586
  37. The results of this study indicated that in the Han Chinese population TPH2 may be a potential susceptibility gene for bipolar disorder. Further studies are needed to validate this association. PMID: 25152196
  38. findings support that TPH2 may play an important functional role in the development of PTSD and contribute to the limited literature regarding the genetic basis and the sex-specific expression of PTSD's symptoms PMID: 25014617
  39. This study showed that an interaction among childhood abuse and genetic variants of TPH2. PMID: 25214390
  40. Data show that a marginal association between TPH2 rs11179023 and symptom improvement was observed in only the group of major depressive disorder patients treated with interpersonal counseling. PMID: 24958631
  41. Tryptophan hydroxylase 2 gene allele variants might be risk factors for psychiatric conditions in TLE [temporal lobe epilepsy ]. PMID: 24491795
  42. findings suggest that greater TPH2 gene expression is the basis for more TPH2 protein in the Dorsal Raphe Nucleus and Median Raphe Nucleus in alcoholics. PMID: 24942188
  43. TPH2 variant and maternal stress and depression are associated with difficulty disengaging attention from fearful facial expressions. PMID: 24304270
  44. Results show that a single nucleotide loss of function polymorphism in tph2 is sufficient to induce gene-dose-dependent cognitive deficits in mice PMID: 24196946
  45. The main finding was that carriers homozygous for the G allele(TPH2 -703 G/T) showed increased target-related induced gamma-band synchronization when combined with the A/A genotype of the (NE) transporter variation. PMID: 23258345
  46. Our findings suggest that common genetic variations of TPH2 are likely to contribute to genetic susceptibility to paranoid schizophrenia in Han Chinese. PMID: 24668623
  47. polymorphic tph2 enzyme has increased activity, but decreased enzyme stability and serotonin production PMID: 24899127
  48. None of the genetic markers within SLC6A4, MAOA, TPH1 and TPH2 were significantly associated with completed suicide or suicide method in the basic association tests. PMID: 23313272
  49. The results of this study showed no association between tryptophan TPH2 gene polymorphisms and the complication of medication overuse headaches in patients with migraines. PMID: 24458851
  50. None of the three gene variants, 5-HTTLPR, COMTval158met and TPH2 G-703T, was associated with long-term response to CBT for SAD. PMID: 24260145
  51. The G allele of rs2129575 of TPH2 is associated with relatively mild clinical symptoms of autism spectrum disorders individuals in our Japanese popula-tion and Korean population PMID: 23992289
  52. correlation between amygdala-hippocampus connectivity and Gray's trait anxiety scores is positive in individuals homozygous for the TPH2 G-allele, while carriers of at least one T-allele show a negative association. PMID: 23684859
  53. The results of this study concluded that rs4570625 (-703 G/T), rs2129575 and rs1386491 play a significant role in GABAergic neurotransmission and may contribute to the sex-specific dysfunction of the GABAergic system in the Prefrontal cortex. PMID: 23552096
  54. The findings suggest that genetic variations of COMT and TPH2 have synergistic effects on social cognition in the general population PMID: 23965265
  55. The association between TPH2 and suicide/alcohol-related suicide remain unclear. PMID: 23995203
  56. results of the present study indicate likely involvement of ITGB3 and TPH2 in the pathophysiology of ASD in the Indian population. PMID: 23628433
  57. identified a regulatory site in the TPH2 promoter region that may be important for ER-induced TPH2 transcriptional activity. PMID: 24033289
  58. Frontal lobe symptoms are significantly associated with TPH2 polymorphisms in Alzheimer's disease patients. PMID: 23334703
  59. Genetic variation at the TPH2 gene appears to affect impulsivity which, in turn, might predispose to the self-induced vomiting eating disorder phenotype PMID: 23239044
  60. Data indicate that the C-terminal domain was the immunodominant part of tryptophan hydroxylase TPH1, the epitopes of tryptophan hydroxylase TPH2 and tyrosine hydroxylase (TH) were mainly located in the N-terminal regulatory domains. PMID: 23182718
  61. A family-based study found no association between the TPH2 (rs4570625 and rs11178997)polymorphisms and attention deficit hyperactivity disorder, and, therefore, does not support the earlier reports that described a significant association. PMID: 23247084
  62. findings suggest that rs4570625 and rs4565946 polymorphisms in TPH2 gene are involved in development of poststroke anxiety in the Han Chinese population. PMID: 22835848
  63. These results suggested that TPH2 gene polymorphisms might be related to attention deficit hyerpactivity disorder (ADHD) symptoms in Korean children. PMID: 23461725
  64. Data indicate that tryptophan hydroxylase 2 Tph2-/- and LIM homeobox transcription factor 1 beta Lmx1b-/- females displayed a change in sexual preference. PMID: 23716677
  65. The present study indicates an association between TPH2 and depressive symptoms that is conditional on prior experience of stressful life events. Further evidence is provided about the role of the environment in genetic vulnerability to depression. PMID: 22868061
  66. Our results indicated that the TPH2 genotype of rs4565946 is a potential genetic risk factor for tic disorder PMID: 23360517
  67. This studty the Tph2KI mice carrying the Tph2(R439H) allele exhibit an increase in AMY-mPFC delta synchrony. PMID: 23467366
  68. These results suggest that the TPH2 polymorphism affects appraisal processes in the case of negative emotions. PMID: 22322887
  69. The common variants within TPH2 promoter are associated with paranoid schizophrenia in Han Chinese. PMID: 22392150
  70. the association of risk for psychiatric disorders with a common TPH2 yin haplotype is related to the inefficient functional engagement of cortical areas involved in cognitive control and alterations in the mode of functional connectivity of dACC pathways. PMID: 22915309
  71. An involvement was found for functional Tph2 polymorphisms in anxiety-related behaviors. PMID: 22491354
  72. Tryptophan hydroxylase has been implicated in impulsivity, aggression and suicidal behavior. PMID: 23032942
  73. variants in the TPH2 gene contributes to risk factors for post traumatic stress disorder symptoms. PMID: 22483952
  74. results suggest that in humans, genetic factors might partly account for variations in serotonin synthesis in the OBFC. PMID: 21747395
  75. This study showed evidence that genotypes in THP2 have some influence on depressive symptoms in women during and after pregnancy PMID: 22721547
  76. the association between TPH2,CO2 reactivity and panic symptoms induced by carbon dioxide in healthy individuals PMID: 22365257
  77. The results of this study reveled that only a minor role of TPH2 haplotypes for schizophrenia. PMID: 22655589
  78. These results suggest that TPH2 polymorphisms enhance autism spectrum disorder susceptibility. PMID: 22698779
  79. relationship between TPH2 SNPs and major depression PMID: 22693556
  80. These data showing the association of polymorphisms in the TPH2 and MTNR1B genes with the progressive subtypes of multiple sclerosis and disability suggest dysregulation in melatonin pathway PMID: 22698518
  81. The results of this study suggested that TPH2 CNVs do not contribute to genetic susceptibility to schizophrenia. PMID: 22342331
  82. Genetic variation in TPH2 was associated with both anger and gray matter concentration in the inferior orbitofrontal cortex and hippocampus. PMID: 22649797
  83. Hopelessness could be a mediating phenotype between the TPH2 gene, depression and suicidal behavior. PMID: 21946031
  84. This study failed to support the tryptophan hydroxylase 2 mutation association to autism spectrum disorders in a Japanese population. PMID: 22361444
  85. Functional characterization of SNP (rs1386493) and its pathological consequences suggest that the TPH2 gene deserves further attention as a potential risk factor for depression. PMID: 20856248
  86. We found that polymorphisms in SLC6A4, TPH2, and SERPINA6 appear to be maternal factors increasing the risk of having a child with facial clefts. PMID: 22072571
  87. Our research has not demonstrated the existence of associations between the eight SNPs of TPH2 and obsessive-compulsive disorder. PMID: 21829912
  88. No differences in genotype distribution in TPH2 SNPs was found between patients with and without suicide attempts. PMID: 21621273
  89. Allelic variation in the promoter region of a gene that encodes tryptophan hydroxylase isoform 2 (TPH2)has been associated with variations in cognitive function and vulnerability to affective spectrum disorders. PMID: 21418063
  90. CC genotype of TPH2 rs2171363, GG genotype of TPH2 rs7305115, CC genotype of HTR2A rs2770304, and CC genotype of HTR2A rs6313 were associated with reduced internalizing scores for children born in the birth weight quartile above the midpoint. PMID: 21969281
  91. The aim of this study is to investigate a possible association between TPH2 gene polymorphisms and the panic disorder comorbidity susceptibility. PMID: 21085052
  92. results support the hypothesis that the TPH2 gene is associated with Late-onset depression. PMID: 21873838
  93. result suggests that TPH2 gene may have a gender dependent effect on susceptibility to MDD but not with its treatment response in Chinese Han population PMID: 21620479
  94. This study suggested that TPH2 variants neither have a major role in major depression,bipolar disorder and schizophrenia nor in response to treatments. PMID: 21396719
  95. The present study suggests that TPH2 contributes distinctively to the inhibition domain of executive function in young Chinese Han population. PMID: 21457069
  96. TPH2 G/T polymorphisms, and presumed loss of enzyme function, were associated with specific aspects of the PWS phenotype. PMID: 21418060
  97. These results suggest that rs4570625 of tryptophan hydroxylase-2 may play an important role in the development of positive symptoms in Han Chinese schizophrenic patients. PMID: 20938755
  98. Future research on TPH2 will provide an entirely new view of 5-HT in brain development and function related to neuropsychiatric disorders. PMID: 21257271
  99. tryptophan hydroxylase 2 polymorphism was associated with a higher risk for anorexia nervosa, eating disorders characterized by self-induced vomiting PMID: 20946355
  100. Results describe associations of tryptophan hydroxylase 1 and 2 gene variants with irritable bowel syndrome-related GI symptoms and stool characteristics. PMID: 21073637

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Involvement in disease Major depressive disorder (MDD); Attention deficit-hyperactivity disorder 7 (ADHD7)
Protein Families Biopterin-dependent aromatic amino acid hydroxylase family
Tissue Specificity Brain specific.
Database Links

HGNC: 20692

OMIM: 607478

KEGG: hsa:121278

STRING: 9606.ENSP00000329093

UniGene: Hs.736576

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