Recombinant Mouse Lipid phosphate phosphohydrolase 1 (Ppap2a)

Code CSB-CF717214MO
MSDS
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names
Plpp1
Uniprot No.
Alternative Names
Plpp1; Hpic53; Lpp1; Ppap2a; Phospholipid phosphatase 1; 35 kDa PAP; mPAP; Hydrogen peroxide-inducible protein 53; Hic53; Lipid phosphate phosphohydrolase 1; PAP2-alpha; Phosphatidate phosphohydrolase type 2a; Phosphatidic acid phosphatase 2a; PAP-2a; PAP2a
Species
Mus musculus (Mouse)
Expression Region
1-283
Target Protein Sequence
MFDKTRLPYVALDVICVLLAGLPFAILTSRHTPFQRGIFCNDDSIKYPYKEDTIPYALLG GIVIPFCIIVMSIGESLSVYFNVLHSNSFVGNPYIATIYKAVGAFLFGVSASQSLTDIAK YTIGSLRPHFLAICNPDWSKINCSDGYIEDYICQGNEEKVKEGRLSFYSGHSSFSMYCML FVALYLQARMKGDWARLLRPMLQFGLIAFSIYVGLSRVSDYKHHWSDVTVGLIQGAAMAI LVALYVSDFFKDTHSYKERKEEDPHTTLHETASSRNYSTNHEP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Magnesium-independent phospholipid phosphatase of the plasma membrane that catalyzes the dephosphorylation of a variety of glycerolipid and sphingolipid phosphate esters including phosphatidate/PA, lysophosphatidate/LPA, diacylglycerol pyrophosphate/DGPP, sphingosine 1-phosphate/S1P and ceramide 1-phosphate/C1P. Also acts on N-oleoyl ethanolamine phosphate/N-(9Z-octadecenoyl)-ethanolamine phosphate, a potential physiological compound. Through its extracellular phosphatase activity allows both the hydrolysis and the cellular uptake of these bioactive lipid mediators from the milieu, regulating signal transduction in different cellular processes. It is for instance essential for the extracellular hydrolysis of S1P and subsequent conversion into intracellular S1P. Involved in the regulation of inflammation, platelets activation, cell proliferation and migration among other processes. May also have an intracellular activity to regulate phospholipid-mediated signaling pathways.
Gene References into Functions
  1. Data identify a novel role for phospholipid phosphate phosphatase type 1 activity and hepatocyte lysophosphatidic acid levels in glucagon sensitivity via a mechanism involving STAT3. PMID: 28273928
  2. Decreased peritoneal ovarian cancer growth in mice lacking expression of lipid phosphate phosphohydrolase 1. PMID: 25769037
  3. increasing the LPP1 activity in three lines of aggressive cancer cells decreases their abilities to produce tumors and metastases PMID: 25210149
  4. Authors demonstrated for the first time that lipin-1 and PAP2a contribute to macrophage inflammation in response to Francisella tularensis. PMID: 24866789
  5. there are marked differences in biochemical activity between fly Wun and mammalian lipid phosphate phosphatase (LPPs), with Wun having a narrower activity range than has been reported for the mammalian LPPs PMID: 12856002
  6. Lipid phosphate phosphatase 1 (LPP-1) is presumed to regulate the balance between lipid phosphates and their dephosphorylated counterparts PMID: 14687668
  7. LPP1 is a catabolic enzyme for lysophosphatidic acid in vivo. PMID: 19215222

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Subcellular Location
Cell membrane; Multi-pass membrane protein. Apical cell membrane; Multi-pass membrane protein. Membrane raft; Multi-pass membrane protein. Membrane, caveola; Multi-pass membrane protein.
Protein Families
PA-phosphatase related phosphoesterase family
Tissue Specificity
Widely expressed. Highly expressed in kidney and lung. Almost undetectable in brain, heart, bone, muscle or spleen.
Database Links

KEGG: mmu:19012

UniGene: Mm.291029

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