Recombinant Oryza sativa subsp. japonica Vacuolar iron transporter 1.1 (VIT1.1)

Code CSB-CF764556OFG
MSDS
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names
VIT1.1
Uniprot No.
Alternative Names
VIT1; Os04g0463400; LOC_Os04g38940; B1358B12.19; OsJ_15080; Vacuolar iron transporter 1; OsVIT1
Species
Oryza sativa subsp. japonica (Rice)
Expression Region
1-252
Target Protein Sequence
MAAATDGGGLPLLADKAASHSHHHHPERHFTSGEVVRDVIMGVSDGLTVPFALAAGLSGA SAPSSLVLTAGLAEVAAGAISMGLGGYLAAKSEADHYQREMKREQEEIIAVPDTEAAEIG EIMSQYGLEPHEYGPVVDGLRRNPQAWLDFMMRFELGLEKPDPKRAIQSALTIALSYVIG GLVPLLPYMFISTAQNAMLTSVGVTLVALLFFGYIKGRFTGNRPFLSAVQTAIIGALASA AAYGMAKAVQTR
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Vacuolar iron transporter involved in the transfer of iron ions from the cytosol to the vacuole for intracellular iron storage. Vacuolar iron storage is required for seed embryo and seedling development. May be involved in the regulation of iron translocation between flag leaves and seeds. Can transport zinc ions from the cytosol to the vacuole.
Gene References into Functions
  1. OsVIT1 and OsVIT2 are highly expressed in rice flag leaves and function to transport Fe and Zn into vacuoles across the tonoplast. PMID: 22731699
Subcellular Location
Vacuole membrane; Multi-pass membrane protein.
Protein Families
CCC1 family
Tissue Specificity
Highly expressed in leaf blades. Expressed in leaf sheaths.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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