Recombinant Rat Claudin-5 (Cldn5)-VLPs

Code CSB-MP867513RA
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Product Details

Target Names
Cldn5
Uniprot No.
Research Area
Neuroscience
Species
Rattus norvegicus (Rat)
Source
Mammalian cell
Expression Region
1-218aa
Target Protein Sequence
MGSAALEILGLVLCLVGWVGLILACGLPMWQVTAFLDHNIVTAQTTWKGLWMSCVVQSTGHMQCKVYESVLALSAEVQAARALTVGAVLLALVALFVTLTGAQCTTCVAPGPVKARVALTGGALYALCGLLALVPLCWFANIVVREFYDPTVPVSQKYELGAALYIGWAASALLMCGGGLVCCGAWVCTGRPEFSFPVKYSAPRRTTANGDYDKKNYV
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length
Tag Info
C-terminal 10xHis-tagged
If you have specified tag type, please tell us and we will check if it's possible to develop.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from PBS, 6% Trehalose, pH 7.4.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL.Aliquot for long-term storage at -80°C. Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
The VLPs are expressed from human 293 cells (HEK293).Mix the sample gently by repeatedly pipetting it up and down. Do not vortex. Repeated freezing and thawing is not recommended.Store the protein at -20°C/-80°C upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity. The immunization strategy should be optimized (antigen dose, regimen and adjuvant).
Datasheet & COA
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Plays a major role in tight junction-specific obliteration of the intercellular space.
Gene References into Functions
  1. claudin-5 might represent a novel pharmacological target for treating acute lung injury induced by H2S and other hazardous gases. PMID: 28741371
  2. in response to alcohol, increased claudin-5 paradoxically accompanies an increase in paracellular leak and rearrangement of alveolar tight junctions. PMID: 27452368
  3. Immunocytochemistry and Western blotting showed that claudin-5, a critical regulatory protein for tight junctions, was translocated from the membrane to the cytoplasm after cooling in cultured bEnd3 cells PMID: 23653089
  4. Increased claudin-5 and claudin-1 expression and decreased permeability of the blood brain barrier were observed in the hyperbaric oxygenation and cerebral ischemia-reperfusion injury group. PMID: 21626096
  5. Claudin-5 redistribution, mediated by caveolin-1, was observed in blood brain barrier following ischemic stroke. PMID: 22378877
  6. Levels of tight junction protein claudin 5 are decreased in blood-brain barrier cells post-translationally following neurotoxicant administration. PMID: 20970449
  7. Blood-brain barrier permeability increases after stroke through matrix metalloprotein-mediated degradation of claudin-5 and occuludin in tight junctions. PMID: 21717368
  8. Data demonstrate the timing of tight junction-associated proteins claudin-5, occludin, and ZO-1 in light of blood-brain barrier permeability associated with cerebral ischemia reperfusion. PMID: 21318404
  9. These findings suggest that claudin-5 is a main claudin expressed in podocytes and that the formation of tight junctions in the nephrosis may be due to local recruitment of claudin-5 rather than due to total upregulation of the claudin protein levels. PMID: 21271259
  10. claudin-5 and occludin translocate reversibly within cells of the rat blood-optic nerve barrier after borneol treatment PMID: 20473716
  11. Increased buffer concentration by addition of HEPES, MOPS, or TES to the media during differentiation increased independent of the type of buffer. This correlated with increased expression of claudin-5 PMID: 20967520
  12. MUPP1 and claudin-5 colocalized in the incisures, and the COOH-terminal region of claudin-5 interacts with MUPP1 in a PSD-95/Disc Large/zona occludens (ZO)-1 (PDZ)-dependent manner in tight junctions PMID: 12403818
  13. Induction of wild-type claudin-5 is sufficient to reconstitute the paracellular barrier against inulin (5 kDa), but not mannitol (182 Da), in leaky rat lung epithelial cells PMID: 15383327
  14. Data show that adrenomedullin inhibits paracellular transport in a blood-brain barrier in vitro model through claudin-5 overexpression. PMID: 16763778
  15. Claudin 5 is predominantly expressed in brain capillaries, and plays a key role in the appearance of barrier properties of brain capillary endothelial cells. PMID: 16998798
  16. Immunogold electron microscopy demonstrated claudin-5 localized in the tight-junctional fused membranes of adjacent endothelial cells. PMID: 17899156

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Subcellular Location
Cell junction, tight junction. Cell membrane; Multi-pass membrane protein.
Protein Families
Claudin family
Database Links
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