Product Details

Purity

>85% (SDS-PAGE)

Target Names

Cldn5

Uniprot No.

Q9JKD6

Alternative Names

Cldn5; Claudin-5

Species

Rattus norvegicus (Rat)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Plays a major role in tight junction-specific obliteration of the intercellular space.

Gene References into Functions

claudin-5 might represent a novel pharmacological target for treating acute lung injury induced by H2S and other hazardous gases. PMID: 28741371
in response to alcohol, increased claudin-5 paradoxically accompanies an increase in paracellular leak and rearrangement of alveolar tight junctions. PMID: 27452368
Immunocytochemistry and Western blotting showed that claudin-5, a critical regulatory protein for tight junctions, was translocated from the membrane to the cytoplasm after cooling in cultured bEnd3 cells PMID: 23653089
Increased claudin-5 and claudin-1 expression and decreased permeability of the blood brain barrier were observed in the hyperbaric oxygenation and cerebral ischemia-reperfusion injury group. PMID: 21626096
Claudin-5 redistribution, mediated by caveolin-1, was observed in blood brain barrier following ischemic stroke. PMID: 22378877
Levels of tight junction protein claudin 5 are decreased in blood-brain barrier cells post-translationally following neurotoxicant administration. PMID: 20970449
Blood-brain barrier permeability increases after stroke through matrix metalloprotein-mediated degradation of claudin-5 and occuludin in tight junctions. PMID: 21717368
Data demonstrate the timing of tight junction-associated proteins claudin-5, occludin, and ZO-1 in light of blood-brain barrier permeability associated with cerebral ischemia reperfusion. PMID: 21318404
These findings suggest that claudin-5 is a main claudin expressed in podocytes and that the formation of tight junctions in the nephrosis may be due to local recruitment of claudin-5 rather than due to total upregulation of the claudin protein levels. PMID: 21271259
claudin-5 and occludin translocate reversibly within cells of the rat blood-optic nerve barrier after borneol treatment PMID: 20473716
Increased buffer concentration by addition of HEPES, MOPS, or TES to the media during differentiation increased independent of the type of buffer. This correlated with increased expression of claudin-5 PMID: 20967520
MUPP1 and claudin-5 colocalized in the incisures, and the COOH-terminal region of claudin-5 interacts with MUPP1 in a PSD-95/Disc Large/zona occludens (ZO)-1 (PDZ)-dependent manner in tight junctions PMID: 12403818
Induction of wild-type claudin-5 is sufficient to reconstitute the paracellular barrier against inulin (5 kDa), but not mannitol (182 Da), in leaky rat lung epithelial cells PMID: 15383327
Data show that adrenomedullin inhibits paracellular transport in a blood-brain barrier in vitro model through claudin-5 overexpression. PMID: 16763778
Claudin 5 is predominantly expressed in brain capillaries, and plays a key role in the appearance of barrier properties of brain capillary endothelial cells. PMID: 16998798
Immunogold electron microscopy demonstrated claudin-5 localized in the tight-junctional fused membranes of adjacent endothelial cells. PMID: 17899156

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Subcellular Location

Cell junction, tight junction. Cell membrane; Multi-pass membrane protein.

Protein Families

Claudin family

Database Links

KEGG: rno:65131

STRING: 10116.ENSRNOP00000064048

UniGene: Rn.17420

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Recombinant Rat Claudin-5 (Cldn5), partial

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