Recombinant Saccharomyces cerevisiae GCR1-dependent translation factor 1 (GDT1)

Code CSB-CF336432SVG
MSDS
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names
GDT1
Uniprot No.
Alternative Names
GDT1; YBR187W; YBR1310; GCR1-dependent translation factor 1
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-280
Target Protein Sequence
MGNMIKKASLIALLPLFTAAAAAATDAETSMESGSSSHLKSFLMSVSMIGLSEIGDKTFL IAALMAMRHKRVLVFSAAATSLAIMTILSGVVGHSAVAFLSERYTAFFAGILFLVFGYKL TMEGLEMSKDAGVEEEMAEVEEEIAIKDMNQDMDDVEKGGDTAYDKQLKNASIGKKIVHR IRELASFMFSPVWVQIFLMVFLGELGDRSQISIIAMATDSDYWYVIAGAVIGHAICSGLA VVGGKLLATRISIRTITLASSLLFFIFALMYIYQAFTTQD
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
May be involved in ribosome biogenesis. Has also been proposed to play a role in calcium homeostasis.
Gene References into Functions
  1. Gdt1p,to sustain the Golgi glycosylation process, imports Mn(2+) inside the Golgi lumen when Pmr1p exclusively transports Ca(2+). Gdt1p is a Mn(2+)-sensitive protein whose abundance dependents on the kind of the ion transported by Pmr1p. The aspartic residues of the 2 conserved motifs E-x-G-D-[KR], in the cation binding sites of Gdt1p, play a role in Golgi glycosylation and hence in Mn(2+)/Ca(2+)transport. PMID: 29108953
  2. Gdt1p controls cellular calcium stores and plays a major role in the calcium response induced by osmotic shock when the Golgi calcium pump, Pmr1p, is absent. Furthermore, in the presence of a high concentration of external calcium, Gdt1p is required for glycosylation of carboxypeptidase Y and the glucanosyltransferase Gas1p. PMID: 27075443
Subcellular Location
Vacuole membrane; Multi-pass membrane protein. Golgi apparatus membrane.
Protein Families
GDT1 family
Database Links

KEGG: sce:YBR187W

STRING: 4932.YBR187W

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