Recombinant Saccharomyces cerevisiae Protein ROT1 (ROT1)

Code CSB-CF311811SVG
MSDS
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names
ROT1
Uniprot No.
Alternative Names
ROT1; YMR200W; YM8325.01; Protein ROT1; Reversal of TOR2 lethality protein 1
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
25-256
Target Protein Sequence
EDESNSIYGTWSSKSNQVFTGPGFYDPVDELLIEPSLPGLSYSFTEDGWYEEATYQVSGN PRNPTCPMASLIYQHGTYNISENGTLVLNPIEVDGRQLFSDPCNDDGVSTYSRYNQTETF KEYAVGIDPYHGIYTLQLYQYDGTPMQPLYLAYRPPMMLPTETLNPTSSATSTDDPSSNK KRSLRSLVRRSLENRHKTNAIKRQNTSFLTSNAIWYISAGMLGVGSLLFLAF
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Required for normal levels of the cell wall 1,6-beta-glucan. Involved in a protein folding machinery chaperoning proteins acting in various physiological processes including cell wall synthesis and lysis of autophagic bodies. Controls actin cytoskeleton polarization to the mother-bud neck and CLB2 protein stability.
Gene References into Functions
  1. Rot1 needs the transmembrane Ser250 to interact with other membrane components and to exert its functional role. PMID: 24303792
  2. the role of Rot1 as a chaperone required to ensure proper glycosylation is demonstrated in yeast PMID: 22492205
  3. The results show that two regions, amino acids 26-60 and 200-228, are involved in the post-translational translocation of Rot1 across the endoplasmic reticulum membrane. PMID: 20608986
  4. Rot1p participates in protein folding with Kar2p. PMID: 16567426
  5. study revealed a role for Rot1 in cell morphogenesis during cell cycle progression; functional antagonism between Rot1 and Clb2 is supported by genetic and biochemical data, which indicate that Rot1 is implicated in Clb2 protein degradation PMID: 17606994
  6. Characterization of the post-translational modification, transport and membrane topology of Rot1 is reported. PMID: 17914748
  7. is an essential molecular chaperone in the endoplasmic reticulum PMID: 18508919

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Subcellular Location
Endoplasmic reticulum membrane; Single-pass type I membrane protein.
Protein Families
ROT1 family
Database Links

KEGG: sce:YMR200W

STRING: 4932.YMR200W

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