Recombinant Yersinia enterocolitica Outer membrane virulence protein YopE (yopE)

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Code CSB-CF330087YAQ
Size $878
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
yopE
Uniprot No.
Research Area
Others
Alternative Names
yopE; yop25; Outer membrane virulence protein YopE
Species
Yersinia enterocolitica
Source
in vitro E.coli expression system
Expression Region
1-219aa
Target Protein Sequence
MKISSFISTSLPLPASVSGSSSVGEMSGRSVSQQKSDQYANNLAGRTESPQGSSLASRIIERLSSMAHSVIGFIQRMFSEGSHKPVVTPALTPAQMPSPTSFSDSIKQLAAETLPKYMQQLSSLDAETLQKNHDQFATGSGPLRGSITQCQGLMQFCGGELQAEASAILNTPVCGIPFSQWGTVGGAASAYVASGVDLTQAANEIKGLGQQMQQLLSLM
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
26.9kDa
Protein Length
Full Length
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

Design a plasmid containing the gene sequence encoding the Yersinia enterocolitica yopE (1-219aa) with the N-terminal 6xHis tag. This plasmid also includes other regulatory elements such as a promoter, ribosome binding site (RBS), and a terminator sequence. Linearize the recombinant plasmid using restriction enzymes. Add substrates, energy, amino acids, nucleotides, cofactors, salts, and an inducer IPTG to the E. coli cell-free extract to induce protein expression. This will trigger the promoter and initiate transcription and translation of the yopE gene. Monitor protein synthesis and harvest the reaction when the desired protein yield is achieved. Apply the harvested reaction mixture to an affinity column to obtain the purified yopE protein. Analyze the purified recombinant Yersinia enterocolitica yopE protein using SDS-PAGE to confirm identity and purity. Its purity is over 90%.

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Target Background

Function
Essential virulence determinant; cytotoxic effector, involved in resistance to phagocytosis.
Subcellular Location
Cell outer membrane.
Protein Families
YopE family
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