Recombinant Human Interferon omega-1 protein (IFNW1), partial (Active)

Interferon omega-1 occupies a unique niche within the type I interferon family, sharing antiviral and antiproliferative signaling through the JAK/STAT pathway yet displaying distinct immunomodulatory kinetics compared to IFN-alpha subtypes. This tag-free recombinant human IFNW1 (aa 24–195), expressed in *E. coli*, delivers exceptional potency with an ED50 below 0.01 ng/ml in a TF-1 chemotaxis bioassay, corresponding to a specific activity exceeding 1.0 × 10⁸ IU/mg—providing a reliable reference standard for cytokine detection assays and a robust positive control for cell-based proliferation and activation studies. The endotoxin level of less than 1.0 EU/μg satisfies the stringent criteria required to prevent LPS-driven artifacts in sensitive immune cell functional assays, including PBMC stimulation, JAK/STAT phosphorylation profiling, and antibody validation by ELISA. Purity exceeding 97% by SDS-PAGE, combined with this endotoxin control, supports use in *in vivo* models of infection or tumor biology where dose-attribution confidence is essential.