What are polyclonal antibodies?
A Monoclonal antibody, by contrast, comes from a single antibody producing B cell and therefore only binds with one unique epitope. Each individual antibody in a polyclonal mixture is technically a monoclonal antibody; however, this term generally refers to a process by which the actual B-cell is isolated and fused to an immortal hybridoma cell line so that large quantities of identical antibody can be generated.
Monoclonal antibodies vs Polyclonal antibodies
How to choose an antibody for your research? Monoclonal antibodies or polyconal antibodies, that is a problem. You need to consider their characteristics and your experiment requirements before making the decision.
The following are the advantages and disadvantages of monoclonal antibodies when they are compared with polyclonal antibodies.
Advantages:
Disadvantages:
CD31 Monoclonal Antibody referenced in "Wound healing potential of quercetin-3-O-rhamnoside and myricetin-3-O-rhamnoside isolated from Pistacia lentiscus distilled leaves in rats model", Biomedicine & pharmacotherapy, 2021.
CCL2 Monoclonal Antibody referenced in "Dynein Light Chain LC8 Alleviates Nonalcoholic Steatohepatitis By Regulating Nuclear Factor κB Through Interference With IκBα Phosphorylation", Research square, 2021.
CRP Monoclonal Antibody, SAA1 Monoclonal Antibody referenced in "Combined Detection of C-reactive Protein and Serum Amyloid A Based on Up-conversion Luminescent System for Internet of Medical Things Application", IEEE Transactions on Nanotechnology, 2021.
Enrofloxacin Monoclonal Antibody referenced in "An automated magnetic separation device coupled with a fluorescent biosensor for the detection of antibiotic residues", Am J Cancer Res, 2021.
PD-L1 Monoclonal Antibody referenced in "Expression of STAT1 is positively correlated with PD-L1 in human ovarian cancer", Journal Cancer Biology & Therapy, 2020.
Kanamycin Monoclonal Antibody referenced in "A Novel Method for Antibiotic Detection in Milk Based on Competitive Magnetic Immunodetection", Foods (Basel, Switzerland), 2020.