Code | CSB-E11781h |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of human TLR2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 96 | ||||
Range % | 89-101 | |||||
1:2 | Average % | 94 | ||||
Range % | 88-97 | |||||
1:4 | Average % | 92 | ||||
Range % | 85-97 | |||||
1:8 | Average % | 94 | ||||
Range % | 88-98 |
The recovery of human TLR2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 83 | 89-97 | ||||
EDTA plasma (n=4) | 90 | 85-96 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The human TLR2 ELISA Kit is engineered for accurate measurement of human TLR2 levels from samples including serum, plasma, or tissue homogenates. It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the TLR2 content in the sample. The color intensity is positively correlated with TLR2 content in the sample. The TLR2 concentration can be calculated according to the standard curve. This kit is tested with high sensitivity, strong specificity, good linearity, high precision and recovery, as well as lot-to-lot consistency.
TLR2 plays a major role in Gram-positive bacterial recognition and defense against mycobacterial infection. TLR2 plays a protective role during infection by triggering a strong pro-inflammatory response, which is beneficial for bacterial clearance. However, the excessive inflammation caused by TLR2 can result in tissue damage and even affect the healing of damaged tissues. It has been reported that TLR2 has a broad range of functions in infectious diseases and also in other diseases, such as chronic and acute inflammatory diseases, cancers, and even metabolic disorders.
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