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Product Name:

human toll-like receptor 2,TLR2 ELISA Kit

Product Type : ELISA Kit
Code : CSB-E11781h
Size : 96T,5×96T,10×96T
Uniprot NO. : O60603
Abbreviation : TLR2
Protein Biological Process 1 : Immunity
Alias : CD282, TIL4, toll/interleukin 1 receptor-like 4
Species : Human
Protein Biological Process 3 : Immunity
Sample Types : serum, plasma, tissue homogenates
Detect Range : 0.312 ng/ml-20 ng/ml
Sensitivity : 0.078 ng/ml
Assay Time : 1-5h
Sample Volume : 50-100ul
Detection Wavelengt : 450 nm
Availability : 3-5 working days
Protocol :
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Target details : This protein is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB.
HGNC : 11848
RGD : 735138
MGI : 1346060
Precision :
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity :
To assess the linearity of the assay, samples were spiked with high concentrations of human TLR2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %96
Range %89-101
1:2Average %94
Range %88-97
1:4Average %92
Range %85-97
1:8Average %94
Range %88-98
Recovery :
The recovery of human TLR2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8389-97
EDTA plasma (n=4)9085-96
Typical Data :
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
202.632 2.621 2.627 2.504
102.068 2.056 2.062 1.939
51.151 1.132 1.142 1.019
2.50.673 0.639 0.656 0.533
1.250.427 0.424 0.426 0.303
0.6250.255 0.245 0.250 0.127
0.3120.189 0.177 0.183 0.060
00.125 0.121 0.123  
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