Purity
Greater than 90% as determined by SDS-PAGE.
Activity
Measured by ADP-Glo Kinase detection kit. The EC50 of MUSK is 2.65-3.58 μg/ml.
Research Area
Signal Transduction
Alternative Names
MUSK; Muscle, skeletal receptor tyrosine-protein kinase; Muscle-specific tyrosine-protein kinase receptor; MuSK; Muscle-specific kinase receptor
Species
Homo sapiens (Human)
Source
in vitro E.coli expression system
Expression Region
24-495aa
Target Protein Sequence
LPKAPVITTPLETVDALVEEVATFMCAVESYPQPEISWTRNKILIKLFDTRYSIRENGQLLTILSVEDSDDGIYCCTANNGVGGAVESCGALQVKMKPKITRPPINVKIIEGLKAVLPCTTMGNPKPSVSWIKGDSPLRENSRIAVLESGSLRIHNVQKEDAGQYRCVAKNSLGTAYSKVVKLEVEEESEPEQDTKVFARILRAPESHNVTFGSFVTLHCTATGIPVPTITWIENGNAVSSGSIQESVKDRVIDSRLQLFITKPGLYTCIATNKHGEKFSTAKAAATISIAEWREYCLAVKELFCAKEWLVMEEKTHRGLYRSEMHLLSVPECSKLPSMHWDPTACARLPHLAFPPMTSSKPSVDIPNLPSSSSSSFSVSPTYSMTVIISIMSSFAIFVLLTITTLYCCRRRKQWKNKKRESAAVTLTTLPSELLLDRLHPNPMYQRMPLLLNPKLLSLEYPRNNIEYVRDI
Protein Length
Extracellular Domain
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
Tris-based buffer,50% glycerol
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
Basically, we can dispatch the products out in 1-3
working days after receiving your orders. Delivery time may differ from different purchasing way or
location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with
normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance
and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Recombinant Human Muscle, skeletal receptor tyrosine-protein kinase(MUSK) is a partial-length active protein expressed in vitro E.coli expression system. This protein contains the extracellular domain (24-495aa) of human MUSK and carries an N-terminal 6xHis-tag. Its purity is greater than 90% as determined by SDS-PAGE. And its bio-activity was measured and confirmed through the ADP-Glo Kinase detection kit. The EC50 of MUSK is 2.65-3.58 μg/ml. The recombinant MUSK protein can be used for specific antibody production. Besides, it may find uses in the studies of MUSK-related signal transduction.
MUSK is a receptor tyrosine kinase (RTK) that exerts a critical role in the formation and maintenance of neuromuscular synapses through the agrin/LRP4-dependent MuSK signaling pathway. Mutations in genes including agrin, MuSK, Dok7, rapsyn, or AChR, fail to form proper neuromuscular junctions (NMJs) or maintain them, ultimately cause congenital myasthenia, resulting in muscle weakness and fatigue. Autoantibodies against the AChR, MUSK, or Lrp4 are responsible for Myasthenia gravis (MG).