ADH1A Antibody

Datasheet

Code	CSB-PA001353LA01HU
Size	US$166
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Image	IHC image of CSB-PA001353LA01HU diluted at 1:200 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA001353LA01HU diluted at 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA001353LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) ADH1A Polyclonal antibody

Uniprot No.

P07327

Target Names

ADH1A

Alternative Names

ADH alpha subunit antibody; ADH antibody; ADH1 antibody; ADH1A antibody; ADH1A_HUMAN antibody; Alcohol dehydrogenase 1 (class I), alpha polypeptide antibody; Alcohol dehydrogenase 1 antibody; Alcohol dehydrogenase 1A (class I), alpha polypeptide antibody; Alcohol dehydrogenase 1A antibody; Alcohol dehydrogenase subunit alpha antibody; Aldehyde reductase antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Alcohol dehydrogenase 1A protein (104-253AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The ADH1A Antibody (Product code: CSB-PA001353LA01HU) is Non-conjugated. For ADH1A Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA001353LB01HU	ADH1A Antibody, HRP conjugated	ELISA
FITC	CSB-PA001353LC01HU	ADH1A Antibody, FITC conjugated
Biotin	CSB-PA001353LD01HU	ADH1A Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Gene References into Functions

High ADH expression is associated with Prostate Cancer. PMID: 28870918
Results suggest a potential role of alcohol dehydrogenase (ADH) class I as a marker for renal cell carcinoma (RCC). PMID: 27086037
Data indicate that the up-regulation of aldehyde dehydrogenase-1 (ALDH1) after neoadjuvant chemotherapy (NAC) predicts poor survival in locally advanced breast cancer. PMID: 24762066
Unde-rexpression of the ADH1 gene, which influences the transformation of the extracellular matrix, plays a probable role in the etiology of uterine fibroid. PMID: 23891545
high levels of ADH1 transcription are implicated in fluconazole resistance in C. albicans and that the mRNA expression levels of ADH1 are positively correlated with those of CDR1, CDR2 and FLU1. PMID: 23769565
common ADH variants conferred risk for both schizophrenia in African-Americans and autism in European-Americans. PMID: 23468174
High ADH1 is associated with endometrial cancer. PMID: 20872569
ADH1A variation predisposes to personality traits and substance dependence PMID: 19526455
Structural aspects that influence dimer-tetramer formation. PMID: 12081471
GATA-2 and HNF-3beta regulate the human alcohol dehydrogenase 1A (ADH1A) gene. PMID: 16153155
The oxidative pathway of ethanol metabolism via ADH and ALDH does not play a role in pancreatic carcinogenesis. PMID: 17632320
Alleles of ADH7 SNPs were associated with the early stages of alcohol metabolism, with additional effects in the ADH1A, ADH1B and ADH4 regions. PMID: 19193628
Observational study of healthcare-related. (HuGE Navigator) PMID: 15279067

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Subcellular Location

Cytoplasm.

Protein Families

Zinc-containing alcohol dehydrogenase family

Database Links

HGNC: 249

OMIM: 103700

KEGG: hsa:124

STRING: 9606.ENSP00000209668

UniGene: Hs.654433

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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