ADH1A Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA234270A0HU
Size	US$210
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Image	Western Blot , Positive WB detected in:COLO205 whole cell lysate(30µg), Mouse liver tissue lysate(30µg), Rat liver lysate(30µg) All lanes: ADH1A antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time：120s IHC image of CSB-RA234270A0HU diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA234270A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of HepG2 cell with CSB-RA234270A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing HepG2 cells stained with CSB-RA234270A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Target Background

Product Details

Uniprot No.

P07327

Target Names

ADH1A

Alternative Names

ADH alpha subunit antibody; ADH antibody; ADH1 antibody; ADH1A antibody; ADH1A_HUMAN antibody; Alcohol dehydrogenase 1 (class I), alpha polypeptide antibody; Alcohol dehydrogenase 1 antibody; Alcohol dehydrogenase 1A (class I), alpha polypeptide antibody; Alcohol dehydrogenase 1A antibody; Alcohol dehydrogenase subunit alpha antibody; Aldehyde reductase antibody

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide from human ADH1A protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

23D2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Alcohol dehydrogenase 1A (ADH1A) serves as a critical enzyme in ethanol metabolism and retinol oxidation, playing essential roles in hepatic detoxification pathways and vitamin A homeostasis. As a class I alcohol dehydrogenase alpha subunit, ADH1A participates in the conversion of alcohols to aldehydes, making it a significant target for researchers investigating metabolic disorders, liver function, and signal transduction pathways.

This recombinant monoclonal antibody, clone 23D2, offers the reproducibility and consistency that demanding experimental workflows require. Generated through recombinant technology using a synthesized peptide derived from human ADH1A, this antibody provides sequence-defined specificity that eliminates the lot-to-lot variability often encountered with traditional hybridoma-derived reagents. The rabbit IgG format ensures reliable performance across multiple detection platforms.

Extensive validation demonstrates this antibody's versatility across diverse experimental applications. Western blot analysis confirms detection of the expected 40 kDa band in COLO205 whole cell lysates as well as mouse and rat liver tissue lysates, providing cross-species reactivity for comparative studies. Immunohistochemistry staining has been validated in paraffin-embedded human liver tissue and liver cancer specimens using citrate buffer antigen retrieval, enabling researchers to examine ADH1A expression patterns in both normal and pathological contexts. Immunofluorescence studies in HepG2 hepatocellular carcinoma cells reveal cytoplasmic localization patterns, while flow cytometry analysis demonstrates clear positive population shifts in the same cell line.

The antibody arrives affinity-purified in a glycerol-containing buffer optimized for long-term storage stability. Whether investigating hepatic metabolism, alcohol-related pathologies, or broader signal transduction mechanisms, this antibody delivers the technical performance needed for publication-quality results.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Gene References into Functions

High ADH expression is associated with Prostate Cancer. PMID: 28870918
Results suggest a potential role of alcohol dehydrogenase (ADH) class I as a marker for renal cell carcinoma (RCC). PMID: 27086037
Data indicate that the up-regulation of aldehyde dehydrogenase-1 (ALDH1) after neoadjuvant chemotherapy (NAC) predicts poor survival in locally advanced breast cancer. PMID: 24762066
Unde-rexpression of the ADH1 gene, which influences the transformation of the extracellular matrix, plays a probable role in the etiology of uterine fibroid. PMID: 23891545
high levels of ADH1 transcription are implicated in fluconazole resistance in C. albicans and that the mRNA expression levels of ADH1 are positively correlated with those of CDR1, CDR2 and FLU1. PMID: 23769565
common ADH variants conferred risk for both schizophrenia in African-Americans and autism in European-Americans. PMID: 23468174
High ADH1 is associated with endometrial cancer. PMID: 20872569
ADH1A variation predisposes to personality traits and substance dependence PMID: 19526455
Structural aspects that influence dimer-tetramer formation. PMID: 12081471
GATA-2 and HNF-3beta regulate the human alcohol dehydrogenase 1A (ADH1A) gene. PMID: 16153155
The oxidative pathway of ethanol metabolism via ADH and ALDH does not play a role in pancreatic carcinogenesis. PMID: 17632320
Alleles of ADH7 SNPs were associated with the early stages of alcohol metabolism, with additional effects in the ADH1A, ADH1B and ADH4 regions. PMID: 19193628
Observational study of healthcare-related. (HuGE Navigator) PMID: 15279067

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Subcellular Location

Cytoplasm.

Protein Families

Zinc-containing alcohol dehydrogenase family

Database Links

HGNC: 249

OMIM: 103700

KEGG: hsa:124

STRING: 9606.ENSP00000209668

UniGene: Hs.654433

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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