Human alcohol dehydrogenase,ADH ELISA Kit

Code CSB-E11855h
Size 96T,5×96T,10×96T
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Product Details

Target Name
alcohol dehydrogenase 1A (class I), alpha polypeptide
Alternative Names
ADH alpha subunit ELISA Kit; ADH ELISA Kit; ADH1 ELISA Kit; ADH1A ELISA Kit; ADH1A_HUMAN ELISA Kit; Alcohol dehydrogenase 1 (class I), alpha polypeptide ELISA Kit; Alcohol dehydrogenase 1 ELISA Kit; Alcohol dehydrogenase 1A (class I), alpha polypeptide ELISA Kit; Alcohol dehydrogenase 1A ELISA Kit; Alcohol dehydrogenase subunit alpha ELISA Kit; Aldehyde reductase ELISA Kit
Abbreviation
ADH1A
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates, cell lysates
Detection Range
0.312 mU/mL-20 mU/mL
Sensitivity
0.078 mU/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ADH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %83
Range %80-87
1:2Average %102
Range %97-107
1:4Average %94
Range %90-99
1:8Average %86
Range %83-89
Recovery
The recovery of human ADH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10299-105
EDTA plasma (n=4)9790-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mU/mlOD1OD2AverageCorrected
202.294 2.156 2.225 2.107
101.778 1.698 1.738 1.620
51.253 1.240 1.247 1.129
2.50.797 0.775 0.786 0.668
1.250.485 0.468 0.477 0.359
0.6250.362 0.375 0.369 0.251
0.3120.256 0.262 0.259 0.141
00.119 0.117 0.118
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human ADH1A ELISA Kit was designed for the quantitative measurement of Human ADH1A protein in serum, plasma, cell culture supernates, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.312 mU/mL-20 mU/mL and the sensitivity is 0.078 mU/mL.

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Target Background

Gene References into Functions
  1. High ADH expression is associated with Prostate Cancer. PMID: 28870918
  2. Results suggest a potential role of alcohol dehydrogenase (ADH) class I as a marker for renal cell carcinoma (RCC). PMID: 27086037
  3. Data indicate that the up-regulation of aldehyde dehydrogenase-1 (ALDH1) after neoadjuvant chemotherapy (NAC) predicts poor survival in locally advanced breast cancer. PMID: 24762066
  4. Unde-rexpression of the ADH1 gene, which influences the transformation of the extracellular matrix, plays a probable role in the etiology of uterine fibroid. PMID: 23891545
  5. high levels of ADH1 transcription are implicated in fluconazole resistance in C. albicans and that the mRNA expression levels of ADH1 are positively correlated with those of CDR1, CDR2 and FLU1. PMID: 23769565
  6. common ADH variants conferred risk for both schizophrenia in African-Americans and autism in European-Americans. PMID: 23468174
  7. High ADH1 is associated with endometrial cancer. PMID: 20872569
  8. ADH1A variation predisposes to personality traits and substance dependence PMID: 19526455
  9. Structural aspects that influence dimer-tetramer formation. PMID: 12081471
  10. GATA-2 and HNF-3beta regulate the human alcohol dehydrogenase 1A (ADH1A) gene. PMID: 16153155
  11. The oxidative pathway of ethanol metabolism via ADH and ALDH does not play a role in pancreatic carcinogenesis. PMID: 17632320
  12. Alleles of ADH7 SNPs were associated with the early stages of alcohol metabolism, with additional effects in the ADH1A, ADH1B and ADH4 regions. PMID: 19193628
  13. Observational study of healthcare-related. (HuGE Navigator) PMID: 15279067

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Subcellular Location
Cytoplasm.
Protein Families
Zinc-containing alcohol dehydrogenase family
Database Links

HGNC: 249

OMIM: 103700

KEGG: hsa:124

STRING: 9606.ENSP00000209668

UniGene: Hs.654433

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