ALDH3B1 Antibody

Datasheet

Code	CSB-PA001574LA01HU
Size	$600
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Image	Western Blot Positive WB detected in: RAW264.7 whole cell lysate, MCF7 whole cell lysate, A549 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate All lanes:ALDH3B1 Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 52 kDa Observed band size: 52 kDa IHC image of CSB-PA001574LA01HU diluted at 1:50 and staining in paraffin-embedded human Lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody IHC image of CSB-PA001574LA01HU diluted at 1:150 and staining in paraffin-embedded mouse liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) ALDH3B1 Polyclonal antibody

Uniprot No.

P43353

Target Names

ALDH3B1

Alternative Names

ALDH3B1 antibody; ALDH7Aldehyde dehydrogenase family 3 member B1 antibody; EC 1.2.1.28 antibody; EC 1.2.1.5 antibody; EC 1.2.1.7 antibody; Aldehyde dehydrogenase 7 antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Aldehyde dehydrogenase family 3 member B1 protein

Immunogen Species

Homo sapiens (Human)

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative:0.02% sodium azide
Constituents: PBS containing 50% glycerol pH 7.3

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:2000
IHC	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

CUSABIO is glad to provide a highly specific antibody directed against an epitope of human ALDH3B1 protein. The polyclonal antibody (CSB-PA001574LA01HU) is raised in the rabbit against a peptide mapping within amino acid residues 168-449 of human ALDH3B1. It exists as an unconjugated IgG isotype. Its purity is 95%+ using protein G purified. This ALDH3B1 antibody has undergone rigorous quality validations in ELISA, IF, and WB analyses. And it has been designed to recognize ALDH3B1 from human, mouse, and rat samples. Its target protein plays an important role in the defense against oxidative stress, specifically lipid peroxidation-derived aldehydes.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Oxidizes medium and long chain saturated and unsaturated aldehydes. Metabolizes also benzaldehyde. Low activity towards acetaldehyde and 3,4-dihydroxyphenylacetaldehyde. May not metabolize short chain aldehydes. Can use both NADP(+) and NAD(+) as electron acceptor. May have a protective role against the cytotoxicity induced by lipid peroxidation.

Gene References into Functions

the likely physiological function of ALDH3B1 is to oxidize lipid-derived aldehydes generated in the plasma membrane and not to be involved in the sphingolipid metabolism in the endoplasmic reticulum. PMID: 23721920
protects cells from the damaging effects of oxidative stress PMID: 20699116
The specificity of ALDH3B1 distribution may prove to be directly related to the functional role of this enzyme in human tissues. PMID: 20729348
This study shows for the first time the functionality, expression and protective role of ALDH3B1 and indicates a potential physiological role of ALDH3B1 against oxidative stress. PMID: 17382292
These findings provided convincing evidence that epistasis between the catechol-O-methyltransferase and aldehyde dehydrogenase 3B1 genes plays an important role in the pathogenesis of schizophrenia PMID: 19159868
Initial characterization of human ALDH3B1 suggests a potential physiological role of this protein in oxidative stress processes. PMID: 17382292

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Subcellular Location

Cell membrane; Lipid-anchor. Note=Primarily in the plasma membrane as well as in some punctate structures in the cytoplasm.

Protein Families

Aldehyde dehydrogenase family

Tissue Specificity

Highest expression in kidney and lung.

Database Links

HGNC: 410

OMIM: 600466

KEGG: hsa:221

UniGene: Hs.523841

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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