ATP12A Antibody

Datasheet

Code	CSB-PA002316LA01HU
Size	US$166
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Image	Immunohistochemistry of paraffin-embedded human skin tissue using CSB-PA002316LA01HU at dilution of 1:100 Immunofluorescent analysis of A431 cells using CSB-PA002316LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) ATP12A Polyclonal antibody

Uniprot No.

P54707

Target Names

ATP12A

Alternative Names

ATP12A antibody; ATP1AL1Potassium-transporting ATPase alpha chain 2 antibody; EC 7.2.2.19 antibody; Non-gastric H(+)/K(+) ATPase subunit alpha antibody; Proton pump antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Potassium-transporting ATPase alpha chain 2 protein (171-306AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The ATP12A Antibody (Product code: CSB-PA002316LA01HU) is Non-conjugated. For ATP12A Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA002316LB01HU	ATP12A Antibody, HRP conjugated	ELISA
FITC	CSB-PA002316LC01HU	ATP12A Antibody, FITC conjugated
Biotin	CSB-PA002316LD01HU	ATP12A Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

The catalytic subunit of a H(+)/K(+) ATPase and/or Na(+)/K(+) ATPase pump which transports K(+) ions in exchange for Na(+) and/or H(+) ions across the apical membrane of epithelial cells. Uses ATP as an energy source to pump K(+) ions into the cell while transporting Na(+) and/or H(+) ions to the extracellular compartment. Involved in the maintenance of electrolyte homeostasis through K(+) ion absorption in kidney and colon. In the airway epithelium, may play a primary role in mucus acidification regulating its viscosity and clearance.

Gene References into Functions

ATP12A knockdown or inhibition prevented the IL-13 dependent increase in airway surface liquid (ASL) viscosity but did not alter the ASL pH PMID: 29391451
expressing human ATP12A in cystic fibrosis mouse airways acidified airway surface liquid, impaired defenses, and increased airway bacteria. PMID: 26823428
Genetic association studies show that carriers of ATP12A rs10507337 C allele or CCTTC haplotype had better pattern of myocardial relaxation as compared to non-carriers. PMID: 25366262
No significantly different expression levels could be detected in the various disease states compared to normal tissue. PMID: 22179016
The presence of nongastric H-K-ATPase in rodent prostate apical membranes may indicate its involvement in potassium concentration regulation in secretions of these glands. PMID: 11880279
Stimulation of protein kinase C pathway mediates endocytosis of human nongastric H+-K+-ATPase, ATP1AL1. PMID: 12110518
Expressed in distal part of human colon and rectum, but also in proximal part of colon. Overexpressed in human colorectal adenocarcinomas adenocarcinomas. Astonishing levels of mRNA overexpression in carcinoma. PMID: 12230809
Carboxy-terminus of HKalpha(2) facilitates proper folding of tHKalpha(2)/beta(1) complex allowing translocation of heterodimer to plasma membrane where potassium uptake occurs. Otherwise, alpha/beta complex is destined for degradation. PMID: 15086469
non-gastric H(+)/K(+)ATPase is present in the microvillous plasma membrane of the transporting epithelia of the human placenta PMID: 15135233
Inhibition of the ATP1AL1 isoenzyme of sodium potassium exchanging ATPase by 8-coumestrol is reported. PMID: 19487957
Use of fold recognition methods enables a prediction that the C-terminal domain of the beta subunits of Na,K-ATPase and H,K-ATPase has an immunoglobulin-like fold, which resembles cell adhesion molecules. PMID: 19694409

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Subcellular Location

Apical cell membrane; Multi-pass membrane protein.

Protein Families

Cation transport ATPase (P-type) (TC 3.A.3) family, Type IIC subfamily

Tissue Specificity

Expressed in airway epithelial cells (at protein level). Found in skin and kidney. Detected in prostate basal cells (at protein level). Expression is increased in benign prostate hyperplasia and tumor tissues (at protein level).

Database Links

HGNC: 13816

OMIM: 182360

KEGG: hsa:479

STRING: 9606.ENSP00000218548

UniGene: Hs.147111

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Oxidative Phosphorylation

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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