Product Details

Purity

>85% (SDS-PAGE)

Target Names

ATP12A

Uniprot No.

P54707

Alternative Names

ATP12A; ATP1AL1Potassium-transporting ATPase alpha chain 2; EC 7.2.2.19; Non-gastric H(+)/K(+) ATPase subunit alpha; Proton pump

Species

Homo sapiens (Human)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Related Products

Target Background

Function

The catalytic subunit of a H(+)/K(+) ATPase and/or Na(+)/K(+) ATPase pump which transports K(+) ions in exchange for Na(+) and/or H(+) ions across the apical membrane of epithelial cells. Uses ATP as an energy source to pump K(+) ions into the cell while transporting Na(+) and/or H(+) ions to the extracellular compartment. Involved in the maintenance of electrolyte homeostasis through K(+) ion absorption in kidney and colon. In the airway epithelium, may play a primary role in mucus acidification regulating its viscosity and clearance.

Gene References into Functions

ATP12A knockdown or inhibition prevented the IL-13 dependent increase in airway surface liquid (ASL) viscosity but did not alter the ASL pH PMID: 29391451
expressing human ATP12A in cystic fibrosis mouse airways acidified airway surface liquid, impaired defenses, and increased airway bacteria. PMID: 26823428
Genetic association studies show that carriers of ATP12A rs10507337 C allele or CCTTC haplotype had better pattern of myocardial relaxation as compared to non-carriers. PMID: 25366262
No significantly different expression levels could be detected in the various disease states compared to normal tissue. PMID: 22179016
The presence of nongastric H-K-ATPase in rodent prostate apical membranes may indicate its involvement in potassium concentration regulation in secretions of these glands. PMID: 11880279
Stimulation of protein kinase C pathway mediates endocytosis of human nongastric H+-K+-ATPase, ATP1AL1. PMID: 12110518
Expressed in distal part of human colon and rectum, but also in proximal part of colon. Overexpressed in human colorectal adenocarcinomas adenocarcinomas. Astonishing levels of mRNA overexpression in carcinoma. PMID: 12230809
Carboxy-terminus of HKalpha(2) facilitates proper folding of tHKalpha(2)/beta(1) complex allowing translocation of heterodimer to plasma membrane where potassium uptake occurs. Otherwise, alpha/beta complex is destined for degradation. PMID: 15086469
non-gastric H(+)/K(+)ATPase is present in the microvillous plasma membrane of the transporting epithelia of the human placenta PMID: 15135233
Inhibition of the ATP1AL1 isoenzyme of sodium potassium exchanging ATPase by 8-coumestrol is reported. PMID: 19487957
Use of fold recognition methods enables a prediction that the C-terminal domain of the beta subunits of Na,K-ATPase and H,K-ATPase has an immunoglobulin-like fold, which resembles cell adhesion molecules. PMID: 19694409

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Subcellular Location

Apical cell membrane; Multi-pass membrane protein.

Protein Families

Cation transport ATPase (P-type) (TC 3.A.3) family, Type IIC subfamily

Tissue Specificity

Expressed in airway epithelial cells (at protein level). Found in skin and kidney. Detected in prostate basal cells (at protein level). Expression is increased in benign prostate hyperplasia and tumor tissues (at protein level).

Database Links

HGNC: 13816

OMIM: 182360

KEGG: hsa:479

STRING: 9606.ENSP00000218548

UniGene: Hs.147111

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Recombinant Human Potassium-transporting ATPase alpha chain 2 (ATP12A), partial

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